scholarly journals Types of voltage-dependent calcium channels involved in high potassium depolarization-induced amylase secretion in the exocrine pancreatic tumour cell line AR4-2J

Cell Research ◽  
1998 ◽  
Vol 8 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Zong Jie Cui
Keyword(s):  
Calcium Channels ◽  
Cell Line ◽  
Tumour Cell ◽  
Tumour Cell Line ◽  
Amylase Secretion ◽  
Potassium Depolarization ◽  
High Potassium ◽  
Pancreatic Tumour ◽  
Voltage Dependent ◽  
Voltage Dependent Calcium Channels

Use of piezoelectric shock waves to effect release of amylase from capsules containing the amylase producing rat pancreatic tumour cell line AR42J

1989 ◽  
Vol 3 (5) ◽  
pp. 355-360 ◽  
Author(s):  
B. Jones ◽  
U. McCarthy ◽  
L. Bunni ◽  
L. McHale ◽  
M. Butler ◽  
...  
Keyword(s):  
Shock Waves ◽  
Cell Line ◽  
Tumour Cell ◽  
Tumour Cell Line ◽  
Pancreatic Tumour

Volatile Anesthetics Reduce Low-voltage-activated Calcium Currents in a Thyroid C-Cell Line

1996 ◽  
Vol 85 (5) ◽  
pp. 1167-1175 ◽  
Author(s):  
Thomas S. McDowell ◽  
Joseph J. Pancrazio ◽  
Carl III Lynch
Keyword(s):  
Calcium Channels ◽  
Cell Line ◽  
Low Voltage ◽  
Volatile Anesthetics ◽  
Calcium Currents ◽  
Whole Cell ◽  
Voltage Range ◽  
C Cell ◽  
Voltage Dependent ◽  
Voltage Dependent Calcium Channels

Background Volatile anesthetics may act in part by inhibiting voltage-dependent calcium channels. The effects of several volatile agents on three types of calcium channels in a thyroid C-cell line were examined. Methods Whole-cell calcium currents were recorded using standard patch clamp techniques. Current-voltage relationships were derived before, during, and after application of isoflurane, enflurane, or halothane. Low-voltage-activated (LVA; T type) calcium currents were isolated based on the voltage range of activation. High-voltage-activated (HVA) calcium currents were separated into L and N types using omega-conotoxin GVIA (omega-CTX) and nicardipine. Results All three agents reversibly decreased both LVA and HVA currents at clinically relevant concentrations. Isoflurane and enflurane both reduced peak LVA current more than peak HVA current: -33 +/- 6% (mean +/- SE) versus -22 +/- 4% for 0.71 mM isoflurane (n = 6), and -46 +/- 6% versus -35 +/- 5% for 1.21 mM enflurane (n = 6). In contrast, halothane depressed LVA and HVA currents to a similar extent: -22 +/- 4% versus -29 +/- 3% for 0.65 mM halothane (n = 6). Isoflurane had no effect on LVA whole-cell current kinetics. Pretreatment with either omega-CTX (400 nM) or nicardipine (1 microM) did not change the sensitivity of HVA current to isoflurane. Conclusions Isoflurane and enflurane block LVA calcium channels more potently than either L-type or N-type calcium channels, but halothane shows no such preferential effect. These results may have implications for the mechanism action of volatile anesthetics.

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