Pretransplant Pharmacodynamic Analysis of Immunosuppressive Agents Using CFSE-Based T-Cell Proliferation Assay

2009 ◽  
Vol 86 (3) ◽  
pp. 285-289 ◽  
Author(s):  
Y Kurata ◽  
M Kato ◽  
T Kuzuya ◽  
Y Miwa ◽  
K Iwasaki ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
Immunosuppressive Agents ◽  
T Cell Proliferation ◽  
Proliferation Assay ◽  
Cell Proliferation Assay ◽  
Pharmacodynamic Analysis

scholarly journals Choice of Microcentrifuge Tubes Influences T Cell Proliferation Assay

BioTechniques ◽  
2000 ◽  
Vol 29 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Y. Wei ◽  
R.J. Mrsny ◽  
R.S. Blumberg ◽  
M. Fried ◽  
A.D. Christ
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
T Cell Proliferation ◽  
Proliferation Assay ◽  
Cell Proliferation Assay

scholarly journals Epoprostenol-associated pneumonitis: Diagnostic use of a T-cell proliferation assay

2010 ◽  
Vol 29 (9) ◽  
pp. 1071-1075 ◽  
Author(s):  
Kristina T. Kudelko ◽  
Kari Nadeau ◽  
Ann N. Leung ◽  
Juliana Liu ◽  
François Haddad ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
T Cell Proliferation ◽  
Proliferation Assay ◽  
Cell Proliferation Assay ◽  
Diagnostic Use

Prevalidation of a human T cell proliferation assay to identify immunosuppressive compounds

2011 ◽  
Vol 205 ◽  
pp. S47
Author(s):  
J.P. Ten Klooster ◽  
M. Teunis ◽  
A. Kleensang ◽  
C. Krul ◽  
H. Van Loveren ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
T Cell Proliferation ◽  
Proliferation Assay ◽  
Cell Proliferation Assay ◽  
Human T Cell

scholarly journals Statin Modulation of Human T-Cell Proliferation, IL-1 and IL-17 Production, and IFN- T Cell Expression: Synergy with Conventional Immunosuppressive Agents

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Ashmal Jameel ◽  
Kenneth G.-J. Ooi ◽  
Natasha R. Jeffs ◽  
Grazyna Galatowicz ◽  
Susan L. Lightman ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
Immunosuppressive Agents ◽  
Experimental Models ◽  
T Cell Proliferation ◽  
Reductase Inhibitors ◽  
Human T Cell ◽  
Human Immune ◽  
Cell Expression

HMG-CoA reductase inhibitors (statins) have been demonstrated to be immunomodulatory for human immune-mediated disease and in experimental models. The aim of this study was to compare statin-mediated immunosuppressive effects on human T-cell responsesin vitrowith those of conventional immunosuppressives (dexamethasone, cyclosporin A (CsA), mycophenolate, and rapamycin). Statins (atorvastatin, lovastatin, and simvastatin) were investigated for their modulatory effects on human PBMC viability, cytokine profiles, and T-cell proliferation. At concentrations that inhibited anti-CD3/28-stimulated T-cell proliferation (), simvastatin significantly decreased intracellular CD4+T-cell expression of IFN- () to levels similar to those induced by conventional immunosuppressives. Atorvastatin and lovastatin also decreased IFN- expression, although to a lesser degree (). All three statins reduced levels of IL-17 production (). However, in response to anti-CD3/28 stimulation, simvastatin significantly upregulated IL-1 production (). The profile of cytokines produced in response to anti-CD3/28 stimulation was similar when both atorvastatin and dexamethasone were added as compared with dexamethasone alone, suggesting that atorvastatin can synergise with dexamethasone with respect to immunomodulation of cytokines. This data supports the hypothesis of selective statin-mediated immunomodulatory effects on human immune cells.

EFFECTS OF LEFLUNOMIDE AND OTHER IMMUNOSUPPRESSIVE AGENTS ON T CELL PROLIFERATION IN VITRO1

1996 ◽  
Vol 61 (1) ◽  
pp. 140-145 ◽  
Author(s):  
Anita S-F. Chong ◽  
Katayoun Rezai ◽  
Howard M. Gebel ◽  
Alison Finnegan ◽  
Preston Foster ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
Immunosuppressive Agents ◽  
T Cell Proliferation
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