Optical Molecular Imaging: From Single Cell to Patient

2008 ◽  
Vol 84 (2) ◽  
pp. 267-271 ◽  
Author(s):  
EH Moriyama ◽  
G Zheng ◽  
BC Wilson
Keyword(s):  
Molecular Imaging ◽  
Single Cell ◽  
Optical Molecular Imaging

scholarly journals Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 499
Author(s):  
Tracy W. Liu ◽  
Seth T. Gammon ◽  
David Piwnica-Worms
Keyword(s):  
Molecular Imaging ◽  
Single Cell ◽  
Real Time ◽  
Bioluminescence Imaging ◽  
Emission Spectra ◽  
Ccd Camera ◽  
Microscopic Imaging ◽  
Signaling Dynamics ◽  
Window Chamber

Intravital microscopic imaging (IVM) allows for the study of interactions between immune cells and tumor cells in a dynamic, physiologically relevant system in vivo. Current IVM strategies primarily use fluorescence imaging; however, with the advances in bioluminescence imaging and the development of new bioluminescent reporters with expanded emission spectra, the applications for bioluminescence are extending to single cell imaging. Herein, we describe a molecular imaging window chamber platform that uniquely combines both bioluminescent and fluorescent genetically encoded reporters, as well as exogenous reporters, providing a powerful multi-plex strategy to study molecular and cellular processes in real-time in intact living systems at single cell resolution all in one system. We demonstrate that our molecular imaging window chamber platform is capable of imaging signaling dynamics in real-time at cellular resolution during tumor progression. Importantly, we expand the utility of IVM by modifying an off-the-shelf commercial system with the addition of bioluminescence imaging achieved by the addition of a CCD camera and demonstrate high quality imaging within the reaches of any biology laboratory.

NAOMI: nanoparticle assisted optical molecular imaging

2006 ◽  
Author(s):  
Dirk J. Faber ◽  
Mirjam E. J. van Velthoven ◽  
Martijn de Bruin ◽  
Maurice C. G. Aalders ◽  
Frank D. Verbraak ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Optical Molecular Imaging

Activatable Fluorescent Probes: A New Concept in Optical Molecular Imaging

2012 ◽  
Vol 19 (28) ◽  
pp. 4731-4741 ◽  
Author(s):  
E. Lacivita ◽  
M. Leopoldo ◽  
F. Berardi ◽  
N. A. Colabufo ◽  
R. Perrone
Keyword(s):  
Molecular Imaging ◽  
Fluorescent Probes ◽  
Optical Molecular Imaging

scholarly journals H-Type Dimer Formation of Fluorophores: A Mechanism for Activatable, in Vivo Optical Molecular Imaging

2009 ◽  
Vol 4 (7) ◽  
pp. 535-546 ◽  
Author(s):  
Mikako Ogawa ◽  
Nobuyuki Kosaka ◽  
Peter L. Choyke ◽  
Hisataka Kobayashi
Keyword(s):  
Molecular Imaging ◽  
Dimer Formation ◽  
Optical Molecular Imaging
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