scholarly journals Preclinical evaluation of a gene therapy treatment for transitional cell carcinoma

2010 ◽  
Vol 18 (1) ◽  
pp. 34-41 ◽  
Author(s):  
X Zhang ◽  
W T Godbey
Keyword(s):  
Gene Therapy ◽  
Transitional Cell Carcinoma ◽  
Cell Carcinoma ◽  
Transitional Cell ◽  
Preclinical Evaluation ◽  
Therapy Treatment

Repeated Intravesical Instillations of an Adenoviral Vector in Patients With Locally Advanced Bladder Cancer: A Phase I Study of p53 Gene Therapy

2003 ◽  
Vol 21 (12) ◽  
pp. 2247-2253 ◽  
Author(s):  
Lance C. Pagliaro ◽  
Afsaneh Keyhani ◽  
Dallas Williams ◽  
Denise Woods ◽  
Baoshun Liu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Bladder Cancer ◽  
Gene Transfer ◽  
Transitional Cell Carcinoma ◽  
Cell Carcinoma ◽  
Locally Advanced ◽  
Transitional Cell ◽  
P53 Gene ◽  
Advanced Bladder Cancer ◽  
Intravesical Instillations

Purpose: We investigated the feasibility, safety, and biologic activity of adenovirus-mediated p53 gene transfer in patients with locally advanced bladder cancer. Patients and Methods: Patients with measurable, locally advanced transitional-cell carcinoma of the bladder who were not candidates for cystectomy were eligible. On a 28-day cycle, intravesical instillations of INGN 201 (Ad5CMV-p53) were administered on days 1 and 4 at three dose levels (1010 particles to 1012 particles) or on either 4 or 8 consecutive days at a single dose level (1012 particles). Results: Thirteen patients received a total of 22 courses without dose-limiting toxicity. Specific transgene expression was detected by reverse transcriptase polymerase chain reaction in bladder biopsy tissue from two of seven assessable patients. There were no changes in p53, p21waf1/cip1, or bax protein levels in bladder epithelium evident from immunohistochemical analysis of 11 assessable patients. Outpatient administration of multiple courses was feasible and well tolerated. A patient with advanced superficial bladder cancer showed evidence of tumor response. Conclusion: Intravesical instillation of Ad5CMV-p53 is safe, feasible, and biologically active when administered in multiple doses to patients with bladder cancer. Observations from this study indicate that this treatment has an antitumor effect in superficial transitional-cell carcinoma. Improvements in the efficiency of gene transfer and the levels of gene expression are required to develop more effective gene therapy for bladder cancer.

Allium sativum Potentiates Suicide Gene Therapy for Murine Transitional Cell Carcinoma

2000 ◽  
Vol 38 (1) ◽  
pp. 98-105 ◽  
Author(s):  
Du-Geon Moon ◽  
Jun Cheon ◽  
Dong-Hee Yoon ◽  
Hong-Seok Park ◽  
Han-Kyeom Kim ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Transitional Cell Carcinoma ◽  
Cell Carcinoma ◽  
Allium Sativum ◽  
Transitional Cell ◽  
Suicide Gene ◽  
Suicide Gene Therapy
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