scholarly journals C-X-C motif chemokine ligand 10 produced by mouse Sertoli cells in response to mumps virus infection induces male germ cell apoptosis

2017 ◽  
Vol 8 (10) ◽  
pp. e3146-e3146 ◽  
Author(s):  
Qian Jiang ◽  
Fei Wang ◽  
Lili Shi ◽  
Xiang Zhao ◽  
Maolei Gong ◽  
...  
Keyword(s):  
Germ Cell ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Mumps Virus ◽  
Male Germ Cell ◽  
Male Germ Cells ◽  
Germ Cell Apoptosis ◽  
Chemokine Ligand

Abstract Mumps virus (MuV) infection usually results in germ cell degeneration in the testis, which is an etiological factor for male infertility. However, the mechanisms by which MuV infection damages male germ cells remain unclear. The present study showed that C-X-C motif chemokine ligand 10 (CXCL10) is produced by mouse Sertoli cells in response to MuV infection, which induces germ cell apoptosis through the activation of caspase-3. CXC chemokine receptor 3 (CXCR3), a functional receptor of CXCL10, is constitutively expressed in male germ cells. Neutralizing antibodies against CXCR3 and an inhibitor of caspase-3 activation significantly inhibited CXCL10-induced male germ cell apoptosis. Furthermore, the tumor necrosis factor-α (TNF-α) upregulated CXCL10 production in Sertoli cells after MuV infection. The knockout of either CXCL10 or TNF-α reduced germ cell apoptosis in the co-cultures of germ cells and Sertoli cells in response to MuV infection. Local injection of MuV into the testes of mice confirmed the involvement of CXCL10 in germ cell apoptosis in vivo. These results provide novel insights into MuV-induced germ cell apoptosis in the testis.

scholarly journals Androgen-dependent apoptosis in male germ cells is regulated through the proto-oncoprotein Cbl

2005 ◽  
Vol 171 (4) ◽  
pp. 651-661 ◽  
Author(s):  
Nisrine El Chami ◽  
Fouziha Ikhlef ◽  
Krisztian Kaszas ◽  
Sadok Yakoub ◽  
Eric Tabone ◽  
...  
Keyword(s):  
Germ Cell ◽  
Germ Cells ◽  
Cell Apoptosis ◽  
Knockout Mouse ◽  
Inhibitor Of Apoptosis ◽  
Male Germ Cells ◽  
Germ Cell Apoptosis ◽  
Androgen Withdrawal ◽  
Hypophysectomized Rats ◽  
Pachytene Spermatocytes

The proto-oncoprotein Cbl is known to control several signaling processes. It is highly expressed in the testis, and because spermatogenesis is androgen dependent, we investigated the androgen dependency expression of Cbl through its testicular sublocalization and its expression levels in rats that were exposed to the antiandrogen flutamide or were hypophysectomized. We report the androgen dependency of Cbl as it localizes in pachytene spermatocytes during androgen-dependent stages, is down-regulated upon flutamide exposure, and is up-regulated with testosterone in hypophysectomized rats. Coculture experiments showed the key control exerted by the Sertoli cell on Cbl activity. As flutamide induces germ cell apoptosis, we investigate members of the Bcl-2 family upon flutamide exposure. We show that the proapoptotic Bcl-2 family member Bim mirrored Cbl expression through a posttranscriptional process. We also show that in Cbl knockout mouse testes, the imbalance between the high expression of Bim and Smac/Diablo and antiapoptotic factors such as cellular inhibitor of apoptosis 2 favors a survival process, which makes these mice unresponsive to androgen withdrawal and could explain their hypofertility.

Oocyte-G1 promotes male germ cell apoptosis through activation of Caspase-3

Gene ◽  
2018 ◽  
Vol 670 ◽  
pp. 22-30 ◽  
Author(s):  
Lin Hou ◽  
Yong Zhang ◽  
Baoli Yu ◽  
Yanzhou Yang ◽  
Bo Li ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Male Germ Cell ◽  
Germ Cell Apoptosis

Minocycline up-regulates BCL-2 levels in mitochondria and attenuates male germ cell apoptosis

2005 ◽  
Vol 337 (2) ◽  
pp. 663-669 ◽  
Author(s):  
Mark Castanares ◽  
Yanira Vera ◽  
Krista Erkkilä ◽  
Sauli Kyttänen ◽  
Yanhe Lue ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Apoptosis ◽  
Male Germ Cell ◽  
Germ Cell Apoptosis

Insights into male germ cell apoptosis due to depletion of gonadotropins caused by GnRH antagonists

APOPTOSIS ◽  
2007 ◽  
Vol 12 (6) ◽  
pp. 1085-1100 ◽  
Author(s):  
Tej K. Pareek ◽  
Ayesha R. Joshi ◽  
Amartya Sanyal ◽  
Rajan R. Dighe
Keyword(s):  
Germ Cell ◽  
Cell Apoptosis ◽  
Male Germ Cell ◽  
Gnrh Antagonists ◽  
Germ Cell Apoptosis

scholarly journals The emerging role of matrix metalloproteases of the ADAM family in male germ cell apoptosis

2011 ◽  
Vol 1 (3) ◽  
pp. 195-208 ◽  
Author(s):  
Ricardo D. Moreno ◽  
Paulina Urriola-Muñoz ◽  
Raúl Lagos-Cabré
Keyword(s):  
Germ Cell ◽  
Cell Apoptosis ◽  
Matrix Metalloproteases ◽  
Male Germ Cell ◽  
Germ Cell Apoptosis ◽  
Adam Family

scholarly journals Overexpression of peroxisomal testis-specific 1 protein induces germ cell apoptosis and leads to infertility in male mice

2011 ◽  
Vol 22 (10) ◽  
pp. 1766-1779 ◽  
Author(s):  
Karina Kaczmarek ◽  
Maja Studencka ◽  
Andreas Meinhardt ◽  
Krzysztof Wieczerzak ◽  
Sven Thoms ◽  
...  
Keyword(s):  
Cell Death ◽  
Germ Cell ◽  
Germ Cells ◽  
Specific Gene ◽  
Male Mice ◽  
Terminal Part ◽  
Male Germ Cells ◽  
Germ Cell Apoptosis ◽  
Induced Apoptosis

 Peroxisomal testis-specific 1 gene (Pxt1) is the only male germ cell–specific gene that encodes a peroxisomal protein known to date. To elucidate the role of Pxt1 in spermatogenesis, we generated transgenic mice expressing a c-MYC-PXT1 fusion protein under the control of the PGK2 promoter. Overexpression of Pxt1 resulted in induction of male germ cells’ apoptosis mainly in primary spermatocytes, finally leading to male infertility. This prompted us to analyze the proapoptotic character of mouse PXT1, which harbors a BH3-like domain in the N-terminal part. In different cell lines, the overexpression of PXT1 also resulted in a dramatic increase of apoptosis, whereas the deletion of the BH3-like domain significantly reduced cell death events, thereby confirming that the domain is functional and essential for the proapoptotic activity of PXT1. Moreover, we demonstrated that PXT1 interacts with apoptosis regulator BAT3, which, if overexpressed, can protect cells from the PXT1-induced apoptosis. The PXT1-BAT3 association leads to PXT1 relocation from the cytoplasm to the nucleus. In summary, we demonstrated that PXT1 induces apoptosis via the BH3-like domain and that this process is inhibited by BAT3.

217 PROTEIN-INDUCED TRANSDIFFERENTIATION INTO MALE GERM CELL-LIKE LINEAGE FROM CHICKEN FETAL BONE MARROW STEM CELLS

2012 ◽  
Vol 24 (1) ◽  
pp. 220
Author(s):  
J. M. Yoo ◽  
J. J. Park ◽  
K. Gobianand ◽  
J. Y. Ji ◽  
J. S. Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Retinoic Acid ◽  
Germ Cell ◽  
Germ Cells ◽  
Cultured Cells ◽  
Male Germ Cell ◽  
Male Germ Cells ◽  
Germ Cell Differentiation ◽  
Transgenic Chickens

Bone marrow (BM)-derived stem cells are capable of transdifferentiation into multilineage cells like muscle, bone, cartilage, fat and nerve cells. In this study, we investigated the capability of mesenchymal stem cells (MSC) derived from BM into germ cell differentiation in the chicken. Chicken MSCs were isolated from BM of day 20 fertilized fetal chicken with Ficoll-Paque Plus. Isolated cells were cultured in advance-DMEM (ADMEM) supplemented with 10% fetal bovine serum and antibiotics. Once confluent, cells were subcultured until five passages. The cultured cells showed fibroblast-like morphology. The cells had positive expressions of Oct4, Sox2 and Nanog. Two induction methods were conducted to examine the ability of transdifferentation into male germ cells. In group 1, MSC were cultured in ADMEM containing retinoic acid and chicken testicular extracts proteins for 10 to 15 days. In group 2, MSC were permeabilized by streptolysin O and treated with chicken testicular protein extracts. In both treatment groups, MSC were cultured in ADMEM containing retinoic acid for 10 to 15 days. We found that chicken MSC had a positive expression of pluripotent proteins such as Oct4, Sox2, Nanog and a small population of chicken MSC seem to transdifferentiate into male germ cell-like cells. These cells expressed early germ cell markers and male germ-cell-specific markers (Dazl, C-kit, Stra8 and DDX4) as analysed by reverse transcription-PCR and immunohistochemistry. These results demonstrated that chicken MSC may differentiate into male germ cells and the same might be used as a potential source of cells for production of transgenic chickens. This study was carried out with the support of Agenda Program (Project No. PJ0064692011), RDA and Republic of Korea.

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