scholarly journals NOX1-induced accumulation of reactive oxygen species in abdominal fat-derived mesenchymal stromal cells impinges on long-term proliferation

2015 ◽  
Vol 6 (4) ◽  
pp. e1728-e1728 ◽  
Author(s):  
M Sela ◽  
G Tirza ◽  
O Ravid ◽  
I Volovitz ◽  
I Solodeev ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Reactive Oxygen ◽  
Abdominal Fat ◽  
Oxygen Species

The impact of mitochondria and reactive oxygen species on the proliferation of human amniotic mesenchymal stromal cells

2018 ◽  
Vol 1859 ◽  
pp. e45
Author(s):  
Sergiu Dumitrescu ◽  
Adelheid Weidinger ◽  
Asmita Banerjee ◽  
Susanne Wolbank ◽  
Karlheinz Hilber ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
The Impact

scholarly journals High inductive magnetic stimuli and their effects on mesenchymal stromal cells, dendritic cells, and fibroblasts

2019 ◽  
pp. S433-S443
Author(s):  
J. Průcha ◽  
J. Skopalik ◽  
I. Justan ◽  
T. Parák ◽  
E. Gabrielová ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Dendritic Cells ◽  
Cell Migration ◽  
Electromagnetic Fields ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Functional Changes

Effects of low-frequency electromagnetic fields (LF EMF) on the activation of different tissue recovery processes have already been fully understood. Preliminary recent data demonstrated that a special case of sinusoidal electromagnetic fields, known as amplitude-modulated currents (AMC) could have a potential to accelerate the cell metabolism or cell migration. An AMC generator was designed to generate sinusoidal induced electric currents with the amplitude modulation and the harmonic carrier frequency of 5,000 Hz was modulated by frequencies of 1 to 100 Hz. The magnetic field peak was 6 mT, electric field intensity 2 V/m and the current density of induced electrical currents was approximately 1 A/m2. The coil of the generator was adapted to easy handling and safe integration into the shelf of the CO2 incubator. The shelf with the coil was prepared for the introduction of cells in standard plastic in vitro chambers. The tests focused on cells with migratory capacity after injury or during immunological processes and thus, mesenchymal stromal cells (MSC), dendritic cells (DC), and fibroblasts were chosen. The tests involved exposures of the cells to LF EMF (180 min/day) every day, for a period of three days, before examining them for cell death, morphology changes, and CD markers. The samples were tested by using MTT assay and the effects on the intracellular concentration of reactive oxygen species were quantified. The cell migration was finally measured with the help of the transwell migration assay. None of the cell types showed any decrease in the cell viability after the LF EMF application and the cells displayed minimum changes in reactive oxygen species. Functional changes (acceleration of cell migration) after AMC exposure were statistically significant for the MSC samples only. The acceleration of MSCs is associated with the production of MMP by these cells. The EMF has a potential to be a safe, clinically applicable selective activator of MSC homing, MSC paracrine production, and subsequent regeneration processes.

scholarly journals Therapeutics effect of mesenchymal stromal cells in reactive oxygen species-induced damages

Human Cell ◽  
2021 ◽  
Author(s):  
Subodh Kumar ◽  
Ranjan Verma ◽  
Nishant Tyagi ◽  
Gurudutta Gangenahalli ◽  
Yogesh Kumar Verma
Keyword(s):  
Reactive Oxygen Species ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Sucralose promotes accumulation of reactive oxygen species (ROS) and adipogenesis in mesenchymal stromal cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Nabanita Kundu ◽  
Cleyton C. Domingues ◽  
Jay Patel ◽  
Mohammed Aljishi ◽  
Neeki Ahmadi ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Effect of Diphenyleneiodonium Chloride on Intracellular Reactive Oxygen Species Metabolism with Emphasis on NADPH Oxidase and Mitochondria in Two Therapeutically Relevant Human Cell Types

Pharmaceutics ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 10
Author(s):  
Sergejs Zavadskis ◽  
Adelheid Weidinger ◽  
Dominik Hanetseder ◽  
Asmita Banerjee ◽  
Cornelia Schneider ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Mesenchymal Stromal Cells ◽  
Mitochondrial Function ◽  
Stromal Cells ◽  
Reactive Oxygen ◽  
Cell Types ◽  
Human Amniotic Membrane ◽  
Osteosarcoma Cell ◽  
Oxygen Species ◽  
Ros Metabolism

Reactive oxygen species (ROS) have recently been recognized as important signal transducers, particularly regulating proliferation and differentiation of cells. Diphenyleneiodonium (DPI) is known as an inhibitor of the nicotinamide adenine dinucleotide phosphate oxidase (NOX) and is also affecting mitochondrial function. The aim of this study was to investigate the effect of DPI on ROS metabolism and mitochondrial function in human amniotic membrane mesenchymal stromal cells (hAMSCs), human bone marrow mesenchymal stromal cells (hBMSCs), hBMSCs induced into osteoblast-like cells, and osteosarcoma cell line MG-63. Our data suggested a combination of a membrane potential sensitive fluorescent dye, tetramethylrhodamine methyl ester (TMRM), and a ROS-sensitive dye, CM-H2DCFDA, combined with a pretreatment with mitochondria-targeted ROS scavenger MitoTEMPO as a good tool to examine effects of DPI. We observed critical differences in ROS metabolism between hAMSCs, hBMSCs, osteoblast-like cells, and MG-63 cells, which were linked to energy metabolism. In cell types using predominantly glycolysis as the energy source, such as hAMSCs, DPI predominantly interacted with NOX, and it was not toxic for the cells. In hBMSCs, the ROS turnover was influenced by NOX activity rather than by the mitochondria. In cells with aerobic metabolism, such as MG 63, the mitochondria became an additional target for DPI, and these cells were prone to the toxic effects of DPI. In summary, our data suggest that undifferentiated cells rather than differentiated parenchymal cells should be considered as potential targets for DPI.

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