scholarly journals Modulation of radiochemoimmunotherapy-induced B16 melanoma cell death by the pan-caspase inhibitor zVAD-fmk induces anti-tumor immunity in a HMGB1-, nucleotide- and T-cell-dependent manner

2015 ◽  
Vol 6 (5) ◽  
pp. e1761-e1761 ◽  
Author(s):  
N Werthmöller ◽  
B Frey ◽  
R Wunderlich ◽  
R Fietkau ◽  
U S Gaipl
Keyword(s):  
Cell Death ◽  
T Cell ◽  
Tumor Immunity ◽  
Melanoma Cell ◽  
B16 Melanoma ◽  
Dependent Manner ◽  
Caspase Inhibitor ◽  
B16 Melanoma Cell

scholarly journals Photodynamic treatment induces cell death by apoptosis or autophagy depending on the melanin content in two B16 melanoma cell lines

2012 ◽  
Vol 29 (3) ◽  
pp. 1196-1200 ◽  
Author(s):  
AGNÈS SPARSA ◽  
SOLENN BELLATON ◽  
THOMAS NAVES ◽  
MARIE-ODILE JAUBERTEAU ◽  
JEAN-MARIE BONNETBLANC ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Melanoma Cell ◽  
B16 Melanoma ◽  
Melanin Content ◽  
Photodynamic Treatment ◽  
B16 Melanoma Cell ◽  
Melanoma Cell Lines

YoshixolTR inhibits B16 melanoma cell growth in vivo and induces apoptosis-like (quantum thermodynamic) cell death

1999 ◽  
Vol 33 (2) ◽  
pp. 161-172 ◽  
Author(s):  
Shozo Koyama ◽  
Satoshi Tanaka ◽  
Hisao Haniu ◽  
Yoshihiro Yamaguchi ◽  
Jiro Motoyoshiya
Keyword(s):  
Cell Death ◽  
Cell Growth ◽  
Melanoma Cell ◽  
B16 Melanoma ◽  
B16 Melanoma Cell ◽  
Quantum Thermodynamic

Activation of AMP-activated protein kinase is involved in vincristine-induced cell apoptosis in B16 melanoma cell

2011 ◽  
Vol 226 (7) ◽  
pp. 1915-1925 ◽  
Author(s):  
Min-Bin Chen ◽  
Wen-Xiang Shen ◽  
Yun Yang ◽  
Xiao-Yang Wu ◽  
Jin-Hua Gu ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Melanoma Cell ◽  
Cell Apoptosis ◽  
B16 Melanoma ◽  
B16 Melanoma Cell ◽  
Amp Activated Protein Kinase

scholarly journals 595 Inhibition of integrin αvβ8 in combination with low dose radiation induces antitumor effect in advanced immune checkpoint blockade refractory tumor model

2021 ◽  
Vol 9 (Suppl 3) ◽  
pp. A625-A625
Author(s):  
Natalia Reszka-Blanco ◽  
Megan Krumpoch ◽  
Michaela Mentzer ◽  
Vinod Yadav Yadav ◽  
Brianna Bannister ◽  
...  
Keyword(s):  
Cell Death ◽  
T Cell ◽  
Dendritic Cell ◽  
Tumor Immunity ◽  
Immune Checkpoint ◽  
Low Dose ◽  
Immune Checkpoint Blockade ◽  
Low Dose Radiation ◽  
Dose Radiation ◽  
Ct26 Model

BackgroundIntegrin αvβ8 activates TGFβ in immune cells. αvβ8 inhibitors have been shown to potentiate immune checkpoint blockade (ICB) in preclinical models [1]. Radioimmunotherapy (RIT) induces immunogenic cell death and antigen presentation, however it concurrently activates immunosuppressive pathways. Interestingly, αvβ8 immunosuppressive activity was implicated in radiotherapy resistance [2]. We have explored whether antagonizing αvβ8 overcomes the suppressive effect of TGFβ and restores anti-tumor immunity in advanced ICB and RIT resistant tumors.MethodsEfficacy was evaluated after combination treatment with low dose radiation, αvβ8 (clone C6D4) and PD-1 (clone J43) mAb in an advanced CT26 colon cancer syngeneic mouse model. Mice were treated at tumor volume of >120 mm3 and euthanized at 2,000 mm3. Flow cytometry and transcriptomic analysis were used to assess the mechanism of action. Tumor volumes are presented as mean±SEM. Statistics were performed by one-way ANOVA, or log-rank test. Bone marrow derived dendritic cell (BMdDC) cultures were isolated from C57BL/6 mice.ResultsCell death, including radiation-induced apoptosis, induced immunoregulatory and maturation program in a population of ex vivo cultured BMdDC, recently described as mregDC/DC3 [3,4]. mregDC/DC3 signature was associated with increased αvβ8 expression, suggesting a role of this integrin in inducing an immunosuppressive phenotype.A CT26 model was established to mimic the progression of late-stage tumors and was unresponsive to radiation, ICB and RIT. In CT26 implanted mice, αvβ8 is expressed on tumor stoma, and is not detectable on cancer cells. Addition of αvβ8 mAb to RIT markedly increased tumor regression (P=0.0067) and survival (P<0.0001). There were 8/10 complete responders with addition of αvβ8 mAb relative to 3/10 in RIT alone. Improved efficacy correlated with enhanced T cell activation and improved DC functionality. Consistent with a recent report in a less advanced CT26 model [5], αvβ8 mAb + radiation resulted in similar efficacy as conventional RIT although the effect was modest in more advanced tumors (Figure 1, A, B).Abstract 595 Figure 1Complete response (CR) with improved survival when αvβ8 inhibition is added to RIT in CT26 syngeneic model of colorectal cancer in an advanced, ICB and RIT unresponsive stage. (A) Effect of combination therapy with low dose radiation (small animal radiation research platform (SARRP) at 5 Gray (Gy) on the day of staging (day 10)), PD-1 mAb (10 mg/kg twice weekly for 2 weeks) and αvβ8 mAb (7 mg/kg three times weekly for 3 weeks) measured by tumor burden. 5Gy+PD-1 and 5Gy+αvβ8 has a minimal effect on tumor growth inhibition showing slight improvement relative to radiation alone (5Gy+IgG). Addition of αvβ8 antagonism (5Gy+αvβ8+PD-1) improves anti-tumor responses leading to CR in 8 of 10 mice. (B) Kaplan-Meier Curve presenting time to progression. 5Gy+IgG improved survival over monotherapy with either αvβ8 or PD1 mAb. 5Gy+αvβ8+PD-1 resulted in a profound improvement of the survival over all other treatment conditionsConclusionsInhibition of αvβ8 in combination with RIT eradicated an advanced tumor, unresponsive to the respective monotherapies or conventional RIT. The anti-tumor effect was driven by enhancement of adaptive immunity, improvement of DC function and reduced tumor tolerance. These data provide evidence that αvβ8 inhibition enhances RIT and may be effective against ICB refractory tumors.ReferencesReszka-Blanco NJ,Yadav V, Krumpoch M, Cappellucci L, Cui D, Dowling JE, et al., Inhibition of integrin αvβ8 enhances immune checkpoint induced anti-tumor immunity by acting across immunologic synapse in syngeneic models of breast cancer. AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 1559.Jin S, Lee WC, Aust D, Pilarsky C, Cordes N, β8 integrin mediates pancreatic cancer cell radiochemoresistance. Mol Cancer Res. 2019; 17(10): 2126–2138.Maier B, Leader AM, Chen ST, Tung N, Chang C, LeBerichel J, et al., A conserved dendritic-cell regulatory program limits antitumour immunity. Nature. 2020; 580 (7802): 257–262.Garris CS, Arlauckas SP, Kohler RH, Trefny MP, Garren S, Piot C, Engblom C, et al., Successful anti-PD-1 cancer immunotherapy requires T cell-dendritic cell crosstalk involving the cytokines IFN-γ and IL-12. Immunity. 2018; 49(6): 1148–1161.Dodagatta-Marri E, Ma H-Y, Liang B, Li J, Meyer DS, Chen S-Y, et al., Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy. Cell Report. 2021; 36(1): 109309Ethics ApprovalAll animal work was approved by the site Institutional Animal Care and Use Committee and was performed in conformance with the Guide for the Care and Use of Laboratory Animals within an AAALAC-accredited program. Humane euthanasia criteria were predetermined on the basis of body weight and defined clinical observations.

Beta-adrenergic receptors of B16 melanoma cell

1983 ◽  
Vol 275 (6) ◽  
pp. 415-416 ◽  
Author(s):  
M. Tsuji ◽  
T. Kuno ◽  
C. Tanaka ◽  
M. Ichihashi ◽  
Y. Mishima
Keyword(s):  
Melanoma Cell ◽  
Adrenergic Receptors ◽  
B16 Melanoma ◽  
Beta Adrenergic Receptors ◽  
Beta Adrenergic ◽  
B16 Melanoma Cell

scholarly journals Diterpenoid B derived from Plectranthus excisus inhibits the melanoma cell cycle in the B16 melanoma cell line

2015 ◽  
Vol 12 (3) ◽  
pp. 4578-4583 ◽  
Author(s):  
YA-NAN LIU ◽  
JUN-LIAN GU ◽  
MENG-SHI MA ◽  
HUA GUO ◽  
LONG LIU ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
B16 Melanoma ◽  
B16 Melanoma Cell

Suppressive Mechanism of Salmosin, a Novel Disintegrin in B16 Melanoma Cell Metastasis

2000 ◽  
Vol 275 (1) ◽  
pp. 169-173 ◽  
Author(s):  
In-Cheol Kang ◽  
Doo-Sik Kim ◽  
Yangsoo Jang ◽  
Kwang-Hoe Chung
Keyword(s):  
Melanoma Cell ◽  
B16 Melanoma ◽  
Cell Metastasis ◽  
B16 Melanoma Cell ◽  
Suppressive Mechanism

scholarly journals Ligand-dependent Inhibition of B16 Melanoma Cell Migration and Invasion via Endogenous S1P2G Protein-coupled Receptor

2003 ◽  
Vol 278 (35) ◽  
pp. 32841-32851 ◽  
Author(s):  
Kayo Arikawa ◽  
Noriko Takuwa ◽  
Hironori Yamaguchi ◽  
Naotoshi Sugimoto ◽  
Joji Kitayama ◽  
...  
Keyword(s):  
Cell Migration ◽  
Melanoma Cell ◽  
B16 Melanoma ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
B16 Melanoma Cell ◽  
Dependent Inhibition

scholarly journals Doxorubicin cellular pharmacokinetics and DNA breakage in a multi-drug resistant B16 melanoma cell line

1988 ◽  
Vol 57 (2) ◽  
pp. 142-146 ◽  
Author(s):  
R Supino ◽  
M Mariani ◽  
G Capranico ◽  
A Colombo ◽  
G Parmiani
Keyword(s):  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
B16 Melanoma ◽  
Drug Resistant ◽  
Dna Breakage ◽  
Cellular Pharmacokinetics ◽  
B16 Melanoma Cell

Saikosaponin b2 induces differentiation and apoptosis in cultured b16 melanoma cell line

1997 ◽  
Vol 7 (Supplement 1) ◽  
pp. S99
Author(s):  
Z P Zong ◽  
K Fujikawa-Yamamoto ◽  
M Tanino ◽  
M Murakami ◽  
N Yamaguchi ◽  
...  
Keyword(s):  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
B16 Melanoma ◽  
B16 Melanoma Cell
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