scholarly journals Dysferlin regulates cell membrane repair by facilitating injury-triggered acid sphingomyelinase secretion

2014 ◽  
Vol 5 (6) ◽  
pp. e1306-e1306 ◽  
Author(s):  
A Defour ◽  
J H Van der Meulen ◽  
R Bhat ◽  
A Bigot ◽  
R Bashir ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Acid Sphingomyelinase ◽  
Membrane Repair

scholarly journals Mechanism of Ca2+-triggered ESCRT assembly and regulation of cell membrane repair

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Luana L. Scheffer ◽  
Sen Chandra Sreetama ◽  
Nimisha Sharma ◽  
Sushma Medikayala ◽  
Kristy J. Brown ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Repair

scholarly journals TRIM72 promotes alveolar epithelial cell membrane repair and ameliorates lung fibrosis

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Xiaofei Cong ◽  
Nagaraja Nagre ◽  
Jeremy Herrera ◽  
Andrew C. Pearson ◽  
Ian Pepper ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Membrane ◽  
Lung Fibrosis ◽  
Alveolar Epithelial Cell ◽  
Membrane Repair ◽  
Alveolar Epithelial

scholarly journals Modulation of Wound Healing and Scar Formation by MG53 Protein-mediated Cell Membrane Repair

2015 ◽  
Vol 290 (40) ◽  
pp. 24592-24603 ◽  
Author(s):  
Haichang Li ◽  
Pu Duann ◽  
Pei-Hui Lin ◽  
Li Zhao ◽  
Zhaobo Fan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Membrane ◽  
Scar Formation ◽  
Membrane Repair

scholarly journals Exocytosis of acid sphingomyelinase by wounded cells promotes endocytosis and plasma membrane repair

2010 ◽  
Vol 189 (6) ◽  
pp. 1027-1038 ◽  
Author(s):  
Christina Tam ◽  
Vincent Idone ◽  
Cecilia Devlin ◽  
Maria Cecilia Fernandes ◽  
Andrew Flannery ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Lysosomal Enzyme ◽  
Membrane Integrity ◽  
Acid Sphingomyelinase ◽  
Membrane Repair ◽  
Membrane Injury ◽  
Cellular Survival ◽  
Plasma Membrane Repair ◽  
Membrane Resealing ◽  
Niemann Pick

Rapid plasma membrane resealing is essential for cellular survival. Earlier studies showed that plasma membrane repair requires Ca2+-dependent exocytosis of lysosomes and a rapid form of endocytosis that removes membrane lesions. However, the functional relationship between lysosomal exocytosis and the rapid endocytosis that follows membrane injury is unknown. In this study, we show that the lysosomal enzyme acid sphingomyelinase (ASM) is released extracellularly when cells are wounded in the presence of Ca2+. ASM-deficient cells, including human cells from Niemann-Pick type A (NPA) patients, undergo lysosomal exocytosis after wounding but are defective in injury-dependent endocytosis and plasma membrane repair. Exogenously added recombinant human ASM restores endocytosis and resealing in ASM-depleted cells, suggesting that conversion of plasma membrane sphingomyelin to ceramide by this lysosomal enzyme promotes lesion internalization. These findings reveal a molecular mechanism for restoration of plasma membrane integrity through exocytosis of lysosomes and identify defective plasma membrane repair as a possible component of the severe pathology observed in NPA patients.

scholarly journals MG53 Nucleates Assembly Of Cell Membrane Repair Machinery

2009 ◽  
Vol 96 (3) ◽  
pp. 361a ◽  
Author(s):  
Chuanxi Cai ◽  
Haruko Masumiya ◽  
Noah Weisleder ◽  
Noriyuki Matsuda ◽  
Miyuki Nishi ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Repair

scholarly journals A Decrease in Membrane Tension Precedes Successful Cell-Membrane Repair

2000 ◽  
Vol 11 (12) ◽  
pp. 4339-4346 ◽  
Author(s):  
Tatsuru Togo ◽  
Tatiana B. Krasieva ◽  
Richard A. Steinhardt
Keyword(s):  
Cell Membrane ◽  
Brefeldin A ◽  
Laser Tweezers ◽  
Membrane Tension ◽  
Membrane Repair ◽  
Ringer’S Solution ◽  
3T3 Fibroblasts ◽  
Membrane Tethers ◽  
Membrane Resealing ◽  
Vesicle Pool

We hypothesized that the requirement for Ca2+-dependent exocytosis in cell-membrane repair is to provide an adequate lowering of membrane tension to permit membrane resealing. We used laser tweezers to form membrane tethers and measured the force of those tethers to estimate the membrane tension of Swiss 3T3 fibroblasts after membrane disruption and during resealing. These measurements show that, for fibroblasts wounded in normal Ca2+ Ringer's solution, the membrane tension decreased dramatically after the wounding and resealing coincided with a decrease of ∼60% of control tether force values. However, the tension did not decrease if cells were wounded in a low Ca2+ Ringer's solution that inhibited both membrane resealing and exocytosis. When cells were wounded twice in normal Ca2+ Ringer's solution, decreases in tension at the second wound were 2.3 times faster than at the first wound, correlating well with twofold faster resealing rates for repeated wounds. The facilitated resealing to a second wound requires a new vesicle pool, which is generated via a protein kinase C (PKC)-dependent and brefeldin A (BFA)-sensitive process. Tension decrease at the second wound was slowed or inhibited by PKC inhibitor or BFA. Lowering membrane tension by cytochalasin D treatment could substitute for exocytosis and could restore membrane resealing in low Ca2+ Ringer's solution.

scholarly journals Guardian Function of Mitsugumin 53 in Cell Membrane Repair and Metabolic Syndrome

2015 ◽  
Vol 108 (2) ◽  
pp. 612a
Author(s):  
Hanley Ma ◽  
Jason Liu ◽  
Zehua Bian ◽  
Yuqi Cui ◽  
Xinyu Zhou ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Cell Membrane ◽  
Membrane Repair
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