scholarly journals Regulation of cytochrome P450 2e1 expression by ethanol: role of oxidative stress-mediated pkc/jnk/sp1 pathway

2013 ◽  
Vol 4 (3) ◽  
pp. e554-e554 ◽  
Author(s):  
M Jin ◽  
A Ande ◽  
A Kumar ◽  
S Kumar
Keyword(s):  
Oxidative Stress ◽  
Cytochrome P450 ◽  
Cytochrome P450 2E1

scholarly journals The role of cytochrome P450 2E1 on ethanol-mediated oxidative stress and HIV replication in human monocyte-derived macrophages

2019 ◽  
Vol 17 ◽  
pp. 65-70 ◽  
Author(s):  
Yuqing Gong ◽  
P.S.S. Rao ◽  
Namita Sinha ◽  
Sabina Ranjit ◽  
Theodore J. Cory ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cytochrome P450 ◽  
Human Monocyte ◽  
Cytochrome P450 2E1 ◽  
Hiv Replication

scholarly journals Role of Mitochondrial Cytochrome P450 2E1 in Healthy and Diseased Liver

Cells ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 288
Author(s):  
Julie Massart ◽  
Karima Begriche ◽  
Jessica H. Hartman ◽  
Bernard Fromenty
Keyword(s):  
Oxidative Stress ◽  
Cytochrome P450 ◽  
Liver Disease ◽  
Fatty Liver ◽  
Current Knowledge ◽  
Physiological Role ◽  
Experimental Investigations ◽  
Rat Liver Mitochondria ◽  
Cytochrome P450 2E1

Cytochrome P450 2E1 (CYP2E1) is pivotal in hepatotoxicity induced by alcohol abuse and different xenobiotics. In this setting, CYP2E1 generates reactive metabolites inducing oxidative stress, mitochondrial dysfunction and cell death. In addition, this enzyme appears to play a role in the progression of obesity-related fatty liver to nonalcoholic steatohepatitis. Indeed, increased CYP2E1 activity in nonalcoholic fatty liver disease (NAFLD) is deemed to induce reactive oxygen species overproduction, which in turn triggers oxidative stress, necroinflammation and fibrosis. In 1997, Avadhani’s group reported for the first time the presence of CYP2E1 in rat liver mitochondria, and subsequent investigations by other groups confirmed that mitochondrial CYP2E1 (mtCYP2E1) could be found in different experimental models. In this review, we first recall the main features of CYP2E1 including its role in the biotransformation of endogenous and exogenous molecules, the regulation of its expression and activity and its involvement in different liver diseases. Then, we present the current knowledge on the physiological role of mtCYP2E1, its contribution to xenobiotic biotransformation as well as the mechanism and regulation of CYP2E1 targeting to mitochondria. Finally, we discuss experimental investigations suggesting that mtCYP2E1 could have a role in alcohol-associated liver disease, xenobiotic-induced hepatotoxicity and NAFLD.

scholarly journals Increased Cytotoxicity of Carbon Tetrachloride in a Human Hepatoma Cell Line Overexpressing Cytochrome P450 2E1

2002 ◽  
Vol 30 (4) ◽  
pp. 400-405 ◽  
Author(s):  
S Takahashi ◽  
T Takahashi ◽  
S Mizobuchi ◽  
M Matsumi ◽  
K Morita ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cytochrome P450 ◽  
Carbon Tetrachloride ◽  
Cell Line ◽  
Hepatoma Cell ◽  
Mrna Level ◽  
Hepatoma Cell Line ◽  
Cytochrome P450 2E1 ◽  
Hsp70 Mrna ◽  
Potential Marker

Cytotoxic free radicals generated during the metabolism of carbon tetrachloride by cytochrome P450 2E1 (CYP2E1) are thought to cause hepatotoxicity. Here, the cytotoxic effects of carbon tetrachloride in a liver cell line expressing CYP2E1 (HLE/2E1) are compared with those in the mother cell line (HLE). The effects of carbon tetrachloride on the gene expression of HSP70, a potential marker of oxidative stress, were also examined. The viability of HLE/2E1 cells after exposure to carbon tetrachloride was significantly decreased compared with that of HLE cells. Northern blot analysis revealed that the HSP70 mRNA level was significantly increased after carbon tetrachloride treatment in both cell lines, while the magnitude of its increase was much greater in HLE/2E1 cells than in HLE cells. These results suggest that the oxidative stress induced by CYP2E1 plays an important role in the increase in cytotoxicity of carbon tetrachloride in CYP2E1-overexpressing cells.

Role of Cytochrome P450 2E1 in the Metabolism of Acrylamide and Acrylonitrile in Mice

1999 ◽  
Vol 12 (11) ◽  
pp. 1110-1116 ◽  
Author(s):  
Susan C. J. Sumner ◽  
Timothy R. Fennell ◽  
Timothy A. Moore ◽  
Brian Chanas ◽  
Frank Gonzalez ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 2E1

Role of Medium-Chain Triglycerides in the Alcohol-Mediated Cytochrome P450 2E1 Induction of Mitochondria

2007 ◽  
Vol 31 (10) ◽  
pp. 1660-1668 ◽  
Author(s):  
Charles S. Lieber ◽  
Qi Cao ◽  
Leonore M. DeCarli ◽  
Maria A. Leo ◽  
Ki M. Mak ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 2E1 ◽  
Medium Chain Triglycerides ◽  
Medium Chain
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