scholarly journals The Bcl-2 repertoire of mesothelioma spheroids underlies acquired apoptotic multicellular resistance

2011 ◽  
Vol 2 (6) ◽  
pp. e174-e174 ◽  
Author(s):  
D Barbone ◽  
J A Ryan ◽  
N Kolhatkar ◽  
A D Chacko ◽  
D M Jablons ◽  
...  
Keyword(s):  
Multicellular Resistance

Abstract 5257: Manipulating the Noxa/Bim axis undermines the multicellular resistance of 3D tumor spheroids

2012 ◽  
Author(s):  
Dario Barbone ◽  
Priscilla Cheung ◽  
Tsung-Ming Yang ◽  
David M. Jablons ◽  
David J. Sugarbaker ◽  
...  
Keyword(s):  
Tumor Spheroids ◽  
Multicellular Resistance

scholarly journals Identification of three gene candidates for multicellular resistance in colon carcinoma

2004 ◽  
Vol 46 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Nicholas E. Timmins ◽  
Tina L. Maguire ◽  
Sean M. Grimmond ◽  
Lars K. Nielsen
Keyword(s):  
Colon Carcinoma ◽  
Multicellular Resistance

Knockdown of focal adhesion kinase reverses colon carcinoma multicellular resistance

2009 ◽  
Vol 100 (9) ◽  
pp. 1708-1713 ◽  
Author(s):  
Yu-Ying Chen ◽  
Zhan-Xiang Wang ◽  
Ping-An Chang ◽  
Jian-Jun Li ◽  
Feng Pan ◽  
...  
Keyword(s):  
Focal Adhesion Kinase ◽  
Colon Carcinoma ◽  
Focal Adhesion ◽  
Multicellular Resistance

scholarly journals Vorinostat Eliminates Multicellular Resistance of Mesothelioma 3D Spheroids via Restoration of Noxa Expression

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e52753 ◽  
Author(s):  
Dario Barbone ◽  
Priscilla Cheung ◽  
Sailaja Battula ◽  
Sara Busacca ◽  
Steven G. Gray ◽  
...  
Keyword(s):  
Multicellular Resistance ◽  
3D Spheroids

scholarly journals Gene Expression Analysis of Tumor Spheroids Reveals a Role for Suppressed DNA Mismatch Repair in Multicellular Resistance to Alkylating Agents

2004 ◽  
Vol 24 (15) ◽  
pp. 6837-6849 ◽  
Author(s):  
Giulio Francia ◽  
Shan Man ◽  
Beverly Teicher ◽  
Luigi Grasso ◽  
Robert S. Kerbel
Keyword(s):  
Drug Resistance ◽  
Mismatch Repair ◽  
Dna Mismatch Repair ◽  
Alkylating Agents ◽  
Acquired Resistance ◽  
Dominant Negative ◽  
Tumor Spheroids ◽  
Dna Mismatch ◽  
Multicellular Resistance

ABSTRACT Drug resistance is a major obstacle in the successful treatment of cancer. Thus, elucidation of the mechanisms responsible is a critical first step in trying to prevent or delay such manifestations of resistance. In this regard, three-dimensional multicellular tumor cell spheroids are intrinsically more resistant to virtually all anticancer cytotoxic drugs than conventional monolayer cultures. We have employed the EMT-6 subline PC5T, which forms highly compact spheroids, and differential display to identify candidate genes whose expression differs between monolayer and spheroids. Approximately 5,000 bands were analyzed, revealing 26 to be differentially expressed. Analysis of EMT-6 tumor variants selected in vivo for acquired resistance to alkylating agents identified eight genes whose expression correlated with drug resistance in tumor spheroids. Four genes (encoding Nop56, the NADH SDAP subunit, and two novel sequences) were found to be down-regulated in EMT-6 spheroids and four (encoding 2-oxoglutarate carrier protein, JTV-1, and two novel sequences) were up-regulated. Analysis of the DNA mismatch repair-associated PMS2 gene, which overlaps at the genomic level with the JTV-1 gene, revealed PMS2 mRNA to be down-regulated in tumor spheroids, which was confirmed at the protein level. Analysis of PMS2−/− mouse embryo fibroblasts confirmed a role for PMS2 in sensitivity to cisplatin, and DNA mismatch repair activity was found to be reduced in EMT-6 spheroids compared to monolayers. Dominant negative PMS2 transfection caused increased resistance to cisplatin in EMT-6 and CHO cells. Our results implicate reduced DNA mismatch repair as a determinant factor of reversible multicellular resistance of tumor cells to alkylating agents.

Abstract 696: ASS1, ANXA4 and MVP underlie acquired multicellular resistance of mesothelioma 3D cell cultures

2014 ◽  
Author(s):  
Dario Barbone ◽  
Loes van Dam ◽  
Shu-Dong Zhang ◽  
Jithesh Puthen ◽  
Dean Fennell ◽  
...  
Keyword(s):  
Cell Cultures ◽  
3D Cell Cultures ◽  
Multicellular Resistance
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