scholarly journals Hypoxia-induced mixed-lineage leukemia 1 regulates glioma stem cell tumorigenic potential

2011 ◽  
Vol 19 (3) ◽  
pp. 428-439 ◽  
Author(s):  
J M Heddleston ◽  
Q Wu ◽  
M Rivera ◽  
S Minhas ◽  
J D Lathia ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mixed Lineage Leukemia ◽  
Glioma Stem Cell ◽  
Tumorigenic Potential

Characterization of stem cell–like cancer cells in immune-competent mice

Blood ◽  
2006 ◽  
Vol 108 (12) ◽  
pp. 3906-3912 ◽  
Author(s):  
Jorg A. Kruger ◽  
Charles D. Kaplan ◽  
Yunping Luo ◽  
He Zhou ◽  
Dorothy Markowitz ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Cells ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Side Population ◽  
Carcinoma Cell Lines ◽  
Tumorigenic Potential ◽  
4T1 Cells

AbstractRecently, the cancer stem cell hypothesis has gained significant recognition as the descriptor of tumorigenesis. Although previous studies relied on transplanting human or rat tumor cells into immunecompromised mice, our study used the Hoechst 33342 dye–based side population (SP) technique to isolate and transplant stem cell–like cancer cells (SCLCCs) from the 4T1 and NXS2 murine carcinoma cell lines into the immune-competent microenvironment of syngeneic mice. 4T1 cells displayed an SP of 2% with a Sca-1highc-Kit–CD45– phenotype, whereas NXS2 cells contained an SP of 0.2% with a Sca-1highCD24highc-Kit–CD45–GD high2 phenotype. Reverse transcription–polymerase chain reaction (RT-PCR) further revealed up-regulation in SP cells of ABCG2, Sca-1, Wnt-1, and TGF-β2. Additionally, 4T1 and NXS2 SP cells exhibited increased resistance to chemotherapy, and 4T1 SP cells also showed an increased ability to efflux doxorubicin, which correlated with a selective increase in the percentage of SP cells found in the tumors of doxorubicin-treated mice. Most importantly, SP cells showed a markedly higher repopulation and tumorigenic potential in vivo, which correlated with an increased number of cells in the SP compartment of SP-derived tumors. Taken together, these results show that we successfully characterized SCLCCs from 2 murine carcinoma cell lines in the immune-competent microenvironment of syngeneic mice.

scholarly journals Aberrant chromatin at genes encoding stem cell regulators in human mixed-lineage leukemia

2008 ◽  
Vol 22 (24) ◽  
pp. 3403-3408 ◽  
Author(s):  
M. G. Guenther ◽  
L. N. Lawton ◽  
T. Rozovskaia ◽  
G. M. Frampton ◽  
S. S. Levine ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mixed Lineage Leukemia ◽  
Genes Encoding

Brain-Tumor-Regenerating 3D Scaffold-Based Primary Xenograft Models for Glioma Stem Cell Targeted Drug Screening

2018 ◽  
Vol 5 (1) ◽  
pp. 139-148 ◽  
Author(s):  
Kottarapat Jeena ◽  
Cheripelil Abraham Manju ◽  
Koythatta Meethalveedu Sajesh ◽  
G Siddaramana Gowd ◽  
Thangalazhi Balakrishnan Sivanarayanan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Brain Tumor ◽  
Drug Screening ◽  
Glioma Stem Cell ◽  
3D Scaffold ◽  
Xenograft Models ◽  
Targeted Drug

scholarly journals IMPS-16MESENCHYMAL TYPE GLIOMA STEM CELL EXPRESS ICOS LIGAND AND UPREGULATE ICOS+IL-10+T CELLS

2015 ◽  
Vol 17 (suppl 5) ◽  
pp. v116.3-v116
Author(s):  
Ryoichi Iwata ◽  
Tomoki Ito ◽  
Muneo Inaba ◽  
Taro Koike ◽  
Hideyuki Oshige ◽  
...  
Keyword(s):  
T Cells ◽  
Stem Cell ◽  
Glioma Stem Cell

scholarly journals Current Strategies for Identification of Glioma Stem Cells: Adequate or Unsatisfactory?

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Paola Brescia ◽  
Cristina Richichi ◽  
Giuliana Pelicci
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Large Scale ◽  
Molecular Mechanisms ◽  
Cell Phenotype ◽  
Multiple Tumor ◽  
Early Results ◽  
Tumorigenic Potential ◽  
Tumor Types

Cancer stem cells (CSCs) were isolated in multiple tumor types, including human glioblastomas, and although the presence of surface markers selectively expressed on CSCs can be used to isolate them, no marker/pattern of markers are sufficiently robust to definitively identify stem cells in tumors. Several markers were evaluated for their prognostic value with promising early results, however none of them was proven to be clinically useful in large-scale studies, leading to outstanding efforts to identify new markers. Given the heterogeneity of human glioblastomas further investigations are necessary to identify both cancer stem cell-specific markers and the molecular mechanisms sustaining the tumorigenic potential of these cells to develop tailored treatments. Markers for glioblastoma stem cells such as CD133, CD15, integrin-α6, L1CAM might be informative to identify these cells but cannot be conclusively linked to a stem cell phenotype. Overlap of expression, functional state and morphology of different subpopulations lead to carefully consider the techniques employed so far to isolate these cells. Due to a dearth of methods and markers reliably identifying the candidate cancer stem cells, the isolation/enrichment of cancer stem cells to be therapeutically targeted remains a major challenge.

scholarly journals Identification of antipsychotic drug fluspirilene as a potential anti-glioma stem cell drug

Oncotarget ◽  
2017 ◽  
Vol 8 (67) ◽  
pp. 111728-111741 ◽  
Author(s):  
Yu Dong ◽  
Takuya Furuta ◽  
Hemragul Sabit ◽  
Tomohiro Kitabayashi ◽  
Shabierjiang Jiapaer ◽  
...  
Keyword(s):  
Stem Cell ◽  
Antipsychotic Drug ◽  
Glioma Stem Cell

scholarly journals SOX9-PDK1 axis is essential for glioma stem cell self-renewal and temozolomide resistance

Oncotarget ◽  
2017 ◽  
Vol 9 (1) ◽  
pp. 192-204 ◽  
Author(s):  
Zhen Wang ◽  
Xiaoshan Xu ◽  
Nan Liu ◽  
Yingduan Cheng ◽  
Weilin Jin ◽  
...  
Keyword(s):  
Stem Cell ◽  
Glioma Stem Cell ◽  
Self Renewal

scholarly journals The stem cell factor SALL4 is an essential transcriptional regulator in mixed lineage leukemia-rearranged leukemogenesis

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Lina Yang ◽  
Li Liu ◽  
Hong Gao ◽  
Jaya Pratap Pinnamaneni ◽  
Deepthi Sanagasetti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Transcriptional Regulator ◽  
Mixed Lineage Leukemia

scholarly journals TMIC-14. HYPOXIA INDUCIBLE OLIG2 MEDIATES GLIOMA STEM CELL MIGRATION

2017 ◽  
Vol 19 (suppl_6) ◽  
pp. vi246-vi246
Author(s):  
James Ross ◽  
Monica Chau ◽  
Brandon Miller ◽  
Subhas Mukherjee ◽  
Changming Zhang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Migration ◽  
Glioma Stem Cell ◽  
Stem Cell Migration

scholarly journals Quantitative proteomics and transcriptomics reveals metabolic differences in attracting and non-attracting human-in-mouse glioma stem cell xenografts and stromal cells

2015 ◽  
Vol 8 ◽  
pp. 94-103 ◽  
Author(s):  
Norelle C. Wildburger ◽  
Cheryl F. Lichti ◽  
Richard D. LeDuc ◽  
Mary Schmidt ◽  
Roger A. Kroes ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stromal Cells ◽  
Quantitative Proteomics ◽  
Glioma Stem Cell ◽  
Mouse Glioma
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