scholarly journals Tumour cell survival signalling by the ERK1/2 pathway

2008 ◽  
Vol 16 (3) ◽  
pp. 368-377 ◽  
Author(s):  
K Balmanno ◽  
S J Cook
Keyword(s):  
Cell Survival ◽  
Tumour Cell

The warburg effect and tumour cell survival in human GBMs

2007 ◽  
Vol 30 (4) ◽  
pp. 97 ◽  
Author(s):  
A Wolf ◽  
J Mukherjee ◽  
A Guha
Keyword(s):  
Cytochrome C ◽  
Cell Survival ◽  
Expression Profile ◽  
Tumour Cell ◽  
Warburg Effect ◽  
Glycolytic Enzymes ◽  
Cytochrome C Release ◽  
Apoptotic Pathway ◽  
The Warburg Effect

Introduction: GBMs are resistant to apoptosis induced by the hypoxic microenvironment and standard therapies including radiation and chemotherapy. We postulate that the Warburg effect, a preferential glycolytic phenotype of tumor cells even under aerobic conditions, plays a role in these aberrant pro-survival signals. In this study we quantitatively examined the expression profile of hypoxia-related glycolytic genes within pathologically- and MRI-defined “centre” and “periphery” of GBMs. We hypothesize that expression of hypoxia-induced glycolytic genes, particularly hexokinase 2 (HK2), favours cell survival and modulates resistance to tumour cell apoptosis by inhibiting the intrinsic mitochondrial apoptotic pathway. Methods: GBM patients underwent conventional T1-weighted contrast-enhanced MRI and MR spectroscopy studies on a 3.0T GE scanner, prior to stereotactic sampling (formalin and frozen) from regions which were T1-Gad enhancing (“centre”) and T2-positive, T1-Gad negative (“periphery”). Real-time qRT-PCR was performed to quantify regional gene expression of glycolytic genes including HK2. In vitro functional studies were performed in U87 and U373 GBM cell lines grown in normoxic (21% pO2) and hypoxic (< 1%pO2) conditions, transfected with HK2 siRNA followed by measurement of cell proliferation (BrdU), apoptosis (activated caspase 3/7, TUNEL, cytochrome c release) and viability (MTS assay). Results: There exists a differential expression profile of glycolytic enzymes between the hypoxic center and relatively normoxic periphery of GBMs. Under hypoxic conditions, there is increased expression of HK2 at the mitochondrial membrane in GBM cells. In vitro HK2 knockdown led to decreased cell survival and increased apoptosis via the intrinsic mitochondrial pathway, as seen by increased mitochondrial release of cytochrome-C. Conclusions: Increased expression of HK2 in the centre of GBMs promotes cell survival and confers resistance to apoptosis, as confirmed by in vitro studies. In vivo intracranial xenograft studies with injection of HK2-shRNA are currently being performed. HK2 and possibly other glycolytic enzymes may provide a target for enhanced therapeutic responsiveness thereby improving prognosis of patients with GBMs.

Mitochondrial elongation-mediated glucose metabolism reprogramming is essential for tumour cell survival during energy stress

Oncogene ◽  
2017 ◽  
Vol 36 (34) ◽  
pp. 4901-4912 ◽  
Author(s):  
J Li ◽  
Q Huang ◽  
X Long ◽  
X Guo ◽  
X Sun ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Cell Survival ◽  
Tumour Cell ◽  
Energy Stress ◽  
Mitochondrial Elongation

129: Semaphorin7A increases growth and metastasis of mammary tumours by promoting tumour cell survival and motility

2014 ◽  
Vol 50 ◽  
pp. S28-S29
Author(s):  
R. Garcia-Areas ◽  
S. Libreros ◽  
S. Amat ◽  
C. Castro-Silva ◽  
P. Robinson ◽  
...  
Keyword(s):  
Cell Survival ◽  
Tumour Cell ◽  
Mammary Tumours

scholarly journals Increased expression of LAT1 in basal cell carcinoma – implications for tumour cell survival

2021 ◽  
Author(s):  
S. Prosén ◽  
E. Tina ◽  
A.H. Sneckenborg ◽  
C. Loinder ◽  
O. Seifert ◽  
...  
Keyword(s):  
Basal Cell Carcinoma ◽  
Cell Survival ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Tumour Cell

scholarly journals Binding of eEF1A2 to the RNA-dependent protein kinase PKR modulates its activity and promotes tumour cell survival

2018 ◽  
Vol 119 (11) ◽  
pp. 1410-1420 ◽  
Author(s):  
Alejandro Losada ◽  
María José Muñoz-Alonso ◽  
Marta Martínez-Díez ◽  
Federico Gago ◽  
Juan Manuel Domínguez ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cell Survival ◽  
Tumour Cell ◽  
Dependent Protein Kinase ◽  
Dependent Protein

scholarly journals Tumour cell survival mechanisms in lethal metastatic prostate cancer differ between bone and soft tissue metastases

2013 ◽  
Vol 230 (3) ◽  
pp. 291-297 ◽  
Author(s):  
Canan Akfirat ◽  
Xiaotun Zhang ◽  
Aviva Ventura ◽  
Dror Berel ◽  
Mary E Colangelo ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Soft Tissue ◽  
Cell Survival ◽  
Tumour Cell ◽  
Metastatic Prostate Cancer ◽  
Survival Mechanisms
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