scholarly journals Effects of imatinib mesylate on spontaneous electrical and mechanical activity in smooth muscle of the guinea-pig stomach

2008 ◽  
Vol 154 (2) ◽  
pp. 451-459 ◽  
Author(s):  
H Hashitani ◽  
M Hayase ◽  
H Suzuki
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Imatinib Mesylate ◽  
Mechanical Activity ◽  
Guinea Pig Stomach

scholarly journals Modification by charybdotoxin and apamin of spontaneous electrical and mechanical activity of the circular smooth muscle of the guinea-pig stomach

1993 ◽  
Vol 109 (3) ◽  
pp. 661-666 ◽  
Author(s):  
Keiko Suzuki ◽  
Kaoru M. Ito ◽  
Yukie Minayoshi ◽  
Hikaru Suzuki ◽  
Masahisa Asano ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Mechanical Activity ◽  
Circular Smooth Muscle ◽  
Guinea Pig Stomach

scholarly journals Inhibitory actions of cilostazol on electrical responses of smooth muscle isolated from the guinea-pig stomach antrum

2004 ◽  
Vol 40 (3) ◽  
pp. 111-124 ◽  
Author(s):  
Eri Nakamura ◽  
Ayako Hashimoto ◽  
Yoshihiko Kito ◽  
Hikaru Hashitani ◽  
Toyoki Mori ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Guinea Pig Stomach ◽  
Electrical Responses

scholarly journals Involvement of protein kinase C in the relaxation of acetylcholine-shortened isolated intact smooth muscle cells from the guinea-pig stomach

1995 ◽  
Vol 67 ◽  
pp. 206
Author(s):  
Sanae Nakamura ◽  
Tsuyoshi Isliibashi ◽  
Mitsuo Mita ◽  
Takao Hashimoto ◽  
Kazuhiko Oishi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Kinase C ◽  
Guinea Pig Stomach

Smooth muscle cells from guinea pig stomach possess high-affinity galanin receptors that mediate relaxation

1994 ◽  
Vol 266 (5) ◽  
pp. G839-G845 ◽  
Author(s):  
Z. F. Gu ◽  
T. K. Pradhan ◽  
D. H. Coy ◽  
R. T. Jensen
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Maximal Effect ◽  
Dependent Manner ◽  
High Affinity ◽  
Gastric Smooth Muscle ◽  
Guinea Pig Stomach ◽  
Galanin Receptors

Galanin-like immunoactivity occurs in nerves and plexi in muscle layers throughout gastrointestinal tract including the stomach. Galanin can affect gastric emptying and contraction or relaxation of gastric muscle in different species. The aim of this study was to investigate the direct effect of galanin on dispersed gastric smooth muscle cells and to characterize any galanin receptors that mediated any effect. Dispersed gastric smooth muscle cells were prepared from guinea pig stomach by collagenase digestion. Porcine galanin (p-galanin; 1 microM) did not stimulate contraction when present alone; however, p-galanin (1 microM) inhibited carbachol-induced contraction with a half-maximal effect at 7 nM. p-Galanin (1 microM) increased cellular adenosine 3',5'-cyclic monophosphate (cAMP) content by 10 s and caused a maximal increase of 80% over basal. 125I-galanin (porcine) bound to dispersed cells in a time- and temperature-dependent manner. Binding was saturable, reversible, and specific. Binding of 125I-galanin was inhibited almost equally by porcine and rat galanin (Ki = 6-8 nM) but was not inhibited by the galanin-associated peptide [preprogalanin-(108-123)]. The fragment galanin-(1-16) was equally potent to rat galanin; however, the fragment galanin-(9-29) was 56-fold less potent (Ki = 370 nM). Computer analysis demonstrated there were two binding sites for p-galanin on gastric smooth muscle cells, a high-affinity site (Kd = 2.6 nM) with low capacity (Bmax = 175 fmol/mg protein) and a low-affinity site (Kd = 150 nM) with large capacity (Bmax = 3,611 fmol/mg protein).(ABSTRACT TRUNCATED AT 250 WORDS)

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