scholarly journals Taxol-mediated augmentation of CD95 ligand-induced apoptosis of human malignant glioma cells: association with bcl-2 phosphorylation but neither activation of p53 nor G2/M cell cycle arrest

1998 ◽  
Vol 77 (3) ◽  
pp. 404-411 ◽  
Author(s):  
W Roth ◽  
B Wagenknecht ◽  
C Grimmel ◽  
J Dichgans ◽  
M Weller
Keyword(s):  
Cell Cycle ◽  
Malignant Glioma ◽  
Cell Cycle Arrest ◽  
Glioma Cells ◽  
M Cell ◽  
Cd95 Ligand ◽  
Cycle Arrest ◽  
Human Malignant Glioma ◽  
Malignant Glioma Cells ◽  
Induced Apoptosis

Pisosterol Induces G2/M Cell Cycle Arrest and Apoptosis via the ATM/ATR Signaling Pathway in Human Glioma Cells

2020 ◽  
Vol 20 (6) ◽  
pp. 734-750
Author(s):  
Wallax A.S. Ferreira ◽  
Rommel R. Burbano ◽  
Claudia do Ó. Pessoa ◽  
Maria L. Harada ◽  
Bárbara do Nascimento Borges ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Signaling Pathway ◽  
Glioma Cell ◽  
Molecular Mechanisms ◽  
Glioma Cells ◽  
Dependent Manner ◽  
M Cell ◽  
Trypan Blue Exclusion ◽  
Cycle Arrest

Background: Pisosterol, a triterpene derived from Pisolithus tinctorius, exhibits potential antitumor activity in various malignancies. However, the molecular mechanisms that mediate the pisosterol-specific effects on glioma cells remain unknown. Objective: This study aimed to evaluate the antitumoral effects of pisosterol on glioma cell lines. Methods: The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and trypan blue exclusion assays were used to evaluate the effect of pisosterol on cell proliferation and viability in glioma cells. The effect of pisosterol on the distribution of the cells in the cell cycle was performed by flow cytometry. The expression and methylation pattern of the promoter region of MYC, ATM, BCL2, BMI1, CASP3, CDK1, CDKN1A, CDKN2A, CDKN2B, CHEK1, MDM2, p14ARF and TP53 was analyzed by RT-qPCR, western blotting and bisulfite sequencing PCR (BSP-PCR). Results: Here, it has been reported that pisosterol markedly induced G2/M arrest and apoptosis and decreased the cell viability and proliferation potential of glioma cells in a dose-dependent manner by increasing the expression of ATM, CASP3, CDK1, CDKN1A, CDKN2A, CDKN2B, CHEK1, p14ARF and TP53 and decreasing the expression of MYC, BCL2, BMI1 and MDM2. Pisosterol also triggered both caspase-independent and caspase-dependent apoptotic pathways by regulating the expression of Bcl-2 and activating caspase-3 and p53. Conclusions: It has been, for the first time, confirmed that the ATM/ATR signaling pathway is a critical mechanism for G2/M arrest in pisosterol-induced glioma cell cycle arrest and suggests that this compound might be a promising anticancer candidate for further investigation.

Lipoxygenase inhibitors block CD95 ligand-mediated apoptosis of human malignant glioma cells

FEBS Letters ◽  
1997 ◽  
Vol 409 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Bettina Wagenknecht ◽  
Erich Gulbins ◽  
Florian Lang ◽  
Johannes Dichgans ◽  
Michael Weller
Keyword(s):  
Malignant Glioma ◽  
Glioma Cells ◽  
Lipoxygenase Inhibitors ◽  
Cd95 Ligand ◽  
Human Malignant Glioma ◽  
Malignant Glioma Cells

Hypericin-induced apoptosis of human malignant glioma cells is light-dependent, independent of bcl-2 expression, and does not require wild-type p53

1997 ◽  
Vol 19 (5) ◽  
pp. 456-470 ◽  
Author(s):  
Michael Weller ◽  
Martin Trepel ◽  
Cornelia Grimmel ◽  
Martin Schabet ◽  
Dirk Bremen ◽  
...  
Keyword(s):  
Malignant Glioma ◽  
Glioma Cells ◽  
Wild Type ◽  
Human Malignant Glioma ◽  
Wild Type P53 ◽  
Malignant Glioma Cells ◽  
Induced Apoptosis

Targeting autophagy enhances BO-1051-induced apoptosis in human malignant glioma cells

2011 ◽  
Vol 69 (3) ◽  
pp. 621-633 ◽  
Author(s):  
Pei-Ming Chu ◽  
Li-Hsin Chen ◽  
Ming-Teh Chen ◽  
Hsin-I Ma ◽  
Tsann-Long Su ◽  
...  
Keyword(s):  
Malignant Glioma ◽  
Glioma Cells ◽  
Human Malignant Glioma ◽  
Malignant Glioma Cells ◽  
Induced Apoptosis

scholarly journals Inhibition of c-Jun-N-terminal-Kinase Sensitizes Tumor Cells to CD95-Induced Apoptosis and Induces G2/M Cell Cycle Arrest

2005 ◽  
Vol 65 (15) ◽  
pp. 6780-6788 ◽  
Author(s):  
Christian Kuntzen ◽  
Nilüfer Sonuc ◽  
Enrico N. De Toni ◽  
Christine Opelz ◽  
Simon R. Mucha ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Tumor Cells ◽  
M Cell ◽  
Cycle Arrest ◽  
Induced Apoptosis
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