scholarly journals Combined effect of navelbine with medroxyprogesterone acetate against human breast carcinoma MCF-7 cells in vitro

1998 ◽  
Vol 77 (11) ◽  
pp. 1737-1743 ◽  
Author(s):  
K Sugiyama ◽  
M Shimizu ◽  
T Akiyama ◽  
H Ishida ◽  
M Okabe ◽  
...  
Keyword(s):  
Breast Carcinoma ◽  
Medroxyprogesterone Acetate ◽  
Human Breast Carcinoma ◽  
Combined Effect ◽  
Human Breast ◽  
Mcf 7

scholarly journals Modulation of Adriamycin Resistance in Human Breast Carcinoma MCF-7 Cellsin vitroandin vivoby Medroxyprogesterone Acetate

1994 ◽  
Vol 85 (5) ◽  
pp. 542-549 ◽  
Author(s):  
Hiroyuki Ishida ◽  
Masami Okabe ◽  
Katsushige Gomi ◽  
Ryuya Horiuchi ◽  
Koji Mikami ◽  
...  
Keyword(s):  
Breast Carcinoma ◽  
Medroxyprogesterone Acetate ◽  
Human Breast Carcinoma ◽  
Human Breast ◽  
Mcf 7

Reversal of P-glycoprotein-mediated multidrug resistance in vitro by milbemycin compounds in adriamycin-resistant human breast carcinoma (MCF-7/adr) cells

2010 ◽  
Vol 24 (6) ◽  
pp. 1474-1481 ◽  
Author(s):  
Wensheng Xiang ◽  
Aili Gao ◽  
Hongsheng Liang ◽  
Changyu Li ◽  
Jiguo Gao ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Breast Carcinoma ◽  
Human Breast Carcinoma ◽  
Human Breast ◽  
P Glycoprotein ◽  
Mcf 7

The apoptotic effects of silibinin on MDA-MB-231 and MCF-7 human breast carcinoma cells

2016 ◽  
Vol 36 (6) ◽  
pp. 573-586 ◽  
Author(s):  
D Bayram ◽  
ES Çetin ◽  
M Kara ◽  
M Özgöçmen ◽  
IA Candan
Keyword(s):  
Breast Carcinoma ◽  
Cell Lines ◽  
Human Breast Carcinoma ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Dead Cell ◽  
Human Breast ◽  
Human Breast Carcinoma Cells ◽  
Breast Carcinoma Cell Lines ◽  
Mcf 7

Background: Silibinin is a bioactive flavonolignan extracted from milk thistle, known as Silybum marianum. Silibinin exerts strong antiproliferative, proapoptotic, and anti-inflammatory effects. Many studies have shown that silibinin inhibits experimentally induced malignancies of the liver, prostate, skin, and colon as well as promotes inhibition of the proliferation of cancer cell lines in vitro. This study aimed to investigate the effects of silibinin on the human breast carcinoma cell lines MDA-MB-231 and MCF-7 in monolayer and spheroid cultures. Method: The MDA-MB-231 and MCF-7 cell lines were cultured in both monolayer and spheroid cultures. Cells were treated with silibinin at 24, 48, and 72 h of incubation. The 5-bromo-2′-deoxyuridine labeling index was used to determine the cells of the synthesis phase. Poly-ADP-ribose-polimerase immunohistochemical staining and the terminal deoxynucleotidyl transferase dUTP nick and labeling assay were used to determine the death of cells in both the monolayer and spheroid cultures. Results: An half maximal inhibitory concentration dose of silibinin in MDA-MB-231 and MCF-7 cells was 100 µM/mL at 24, 48, and 72 h of incubation. Terminal deoxynucleotidyl transferase dUTP nick and labeling positive cells and active poly-ADP-ribose-polimerase were detected after treatment with silibinin in both the monolayer and spheroid cultures. The dead cell count was higher in the MDA-MB-231 and MCF-7 cell lines with silibinin applied than in the controls. Conclusions: Our study demonstrated that silibinin applications enhanced terminal deoxynucleotidyl transferase dUTP nick and labeling positive cells and active poly-ADP-ribose-polimerase in comparison to the control in both the monolayer and spheroid cultures.

scholarly journals In Vitro Study on Apoptosis Induced by Strontium-89 in Human Breast Carcinoma Cell Line

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Cheng Wang ◽  
Jing Wang ◽  
Han Jiang ◽  
Min Zhu ◽  
Baoguo Chen ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Breast Carcinoma ◽  
Transmembrane Potential ◽  
Human Breast Carcinoma ◽  
Inhibition Rate ◽  
Human Breast ◽  
Radioactive Concentration ◽  
Mcf 7

Many radiopharmaceuticals used for medical diagnosis and therapy are beta emitters; however, the mechanism of the cell death caused by beta-irradiation is not well understood. The objective of this study was to investigate the apoptosis of human breast carcinoma MCF-7 cell lines induced by Strontium-89 (89Sr) and its regulation and control mechanism. High-metastatic Breast Carcinoma MCF-7 cells were cultured in vitro using89Sr with different radioactive concentration. The inhibition rate of cell proliferation was measured by MTT color matching method. The cell cycle retardation, apoptosis conditions, mitochondrion transmembrane potential difference and Fas expression were tested and analyzed. The genes P53 and bcl-2 expressions was also analyzed using immunity histochemical analysis. After being induced by89Sr with various of radioactive concentration, it was found that the inhibition of cell proliferation of MCF-7 cells was obviously, the retardation of cell cycle occurred mainly in G2-M. It was also found that the obvious apoptosis occurred after being induced by89Sr, the highest apoptosis rate reached 46.28%. The expressions of Fas acceptor and P53 gene increased, while bcl-2 gene expression decreasesd. These findings demonstrate that in the ranges of a certain radioactive concentration, the inhibition rate of MCF-7 cell proliferation and retardation of cell cycle had positive correlation with the concentration of89Sr. And the mitochondrion transmembrane potential decrease would induce the apoptosis of MCF-7 cell notably, which were controlled by P53 and bcl-2 genes, involved with the Fas acceptor.

Cell Surface Characteristics of a Human Breast Carcinoma Cell Line (MCF-7)

1977 ◽  
Vol 35 ◽  
pp. 492-493
Author(s):  
J. Russo ◽  
I. Russo
Keyword(s):  
Breast Carcinoma ◽  
Cell Surface ◽  
Cell Line ◽  
Mammalian Cells ◽  
Ruthenium Red ◽  
Human Breast Carcinoma ◽  
Breast Carcinoma Cell Line ◽  
Human Breast ◽  
Mcf 7

Modifications of the acid mucopolysaccharide coat of mammalian cells is considered an important marker of cell transformation in vitro.In the present paper, the ultrastructural localization of acid mucopolysaccharides on the cell surface of MCF-7 cells is reported. MCF-7 is a cell line derived from a metastatic human breast carcinoma which has been cultured in monolayer in Eagle's minimum essential medium for more than five years. Cells growing in monolayer for more than 142 transfers and cells of the same transfer seeded in collagen-coated cellulose sponges (1) were used in these experiments. Both groups of cells were either rinsed three times in caco- dylate buffer, 0.1 M, pH 7.2, and then fixed and stained with ruthenium red RR (2), or labeled and fixed with cationized ferritin (CF) according to Danon's (3).All the cells growing in monolayer had a continuous electron-dense fuzzy layer of RR attached to the outer leaflet of the cell membrane at both the free surface and the intercellular spaces (Fig. 1).

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