scholarly journals Elevated levels of the angiogenic cytokines basic fibroblast growth factor and vascular endothelial growth factor in sera of cancer patients

1997 ◽  
Vol 76 (2) ◽  
pp. 238-243 ◽  
Author(s):  
LY Dirix ◽  
PB Vermeulen ◽  
A Pawinski ◽  
A Prové ◽  
I Benoy ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Cancer Patients ◽  
Basic Fibroblast Growth Factor ◽  
Vascular Endothelial ◽  
Fibroblast Growth ◽  
Endothelial Growth Factor ◽  
Angiogenic Cytokines

Quantification of vascular endothelial growth factor, interleukin-8, and basic fibroblast growth factor in plasma of cancer patients and healthy volunteers – comparison of ELISA and microsphere-based multiplexed immunoassay

2008 ◽  
Vol 46 (9) ◽  
Author(s):  
Martin Kornacker ◽  
Angelika Roth ◽  
Olaf Christensen ◽  
Jörn Krätzschmar ◽  
Georg Wensing
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factors ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Cancer Patients ◽  
Basic Fibroblast Growth Factor ◽  
Vascular Endothelial ◽  
Fibroblast Growth ◽  
Basic Fgf ◽  
Endothelial Growth Factor

Abstract: Vascular endothelial growth factor (VEGF), interleukin-8 (IL-8) and basic fibroblast growth factor (basic FGF) are angiogenic growth factors which may be useful as biomarkers in drug development, where they could give early information on the antiangiogenic activity of novel anticancer compounds.: We compared two commercially available assays, enzyme linked immunosorbent assay (ELISA) and a multiplexed bead-based immunoassay (xMAP), for the quantification of these factors in plasma samples from more than 100 cancer patients and healthy individuals.: For VEGF and IL-8, but not for basic FGF, xMAP was more sensitive than the respective ELISA. This was true for healthy subjects as well as for cancer patients. Intraassay precision was comparable between both assay formats. Linear regression analysis of VEGF concentrations demonstrated a good correlation between ELISA and xMAP. Bland-Altman analysis showed a systematic difference between both assays, with ELISA giving higher concentration values. VEGF levels were higher in female volunteers, and both assays were able to detect this difference.: Multiplexed microsphere-based immunoassays have the potential to substitute ELISA for the detection of proangiogenic growth factors in clinical studies. Their shorter assay times and their ability to quantify multiple analytes in a small sample volume are advantageous.Clin Chem Lab Med 2008;46:1256–64.

Sign in / Sign up

Export Citation Format

Share Document