scholarly journals Effects of the selective bisindolylmaleimide protein kinase C inhibitor GF 109203X on P-glycoprotein-mediated multidrug resistance

1996 ◽  
Vol 74 (6) ◽  
pp. 897-905 ◽  
Author(s):  
V Gekeler ◽  
R Boer ◽  
F Uberall ◽  
W Ise ◽  
C Schubert ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Multidrug Resistance ◽  
Protein Kinase ◽  
Kinase C ◽  
P Glycoprotein ◽  
Gf 109203X ◽  
Protein Kinase C Inhibitor

scholarly journals P‐Glycoprotein, Multidrug Resistance and Protein Kinase C

1996 ◽  
Vol 1 (4) ◽  
pp. 261-268 ◽  
Author(s):  
Robert L. Fine ◽  
Timothy C. Chambers ◽  
Clifford W. Sachs
Keyword(s):  
Protein Kinase C ◽  
Multidrug Resistance ◽  
Protein Kinase ◽  
Kinase C ◽  
P Glycoprotein

scholarly journals Protein kinase C-mediated phosphorylation of the human multidrug resistance P-glycoprotein regulates cell volume-activated chloride channels.

1995 ◽  
Vol 14 (1) ◽  
pp. 68-75 ◽  
Author(s):  
S.P. Hardy ◽  
H.R. Goodfellow ◽  
M.A. Valverde ◽  
D.R. Gill ◽  
V. Sepúlveda ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Multidrug Resistance ◽  
Protein Kinase ◽  
Cell Volume ◽  
Chloride Channels ◽  
Kinase C ◽  
P Glycoprotein

scholarly journals Protein Kinase C-mediated Phosphorylation Does Not Regulate Drug Transport by the Human Multidrug Resistance P-glycoprotein

1996 ◽  
Vol 271 (23) ◽  
pp. 13668-13674 ◽  
Author(s):  
Hugh R. Goodfellow ◽  
Alessandro Sardini ◽  
Stephan Ruetz ◽  
Richard Callaghan ◽  
Philippe Gros ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Multidrug Resistance ◽  
Protein Kinase ◽  
Drug Transport ◽  
Kinase C ◽  
P Glycoprotein

The bisindolylmaleimide protein kinase C inhibitor, Ro 32-2241, reverses multidrug resistance in KB tumour cells

1999 ◽  
Vol 43 (5) ◽  
pp. 371-378 ◽  
Author(s):  
Janet E. Merritt ◽  
John A. Sullivan ◽  
Lisa Drew ◽  
Abida Khan ◽  
Kevin Wilson ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Multidrug Resistance ◽  
Protein Kinase ◽  
Tumour Cells ◽  
Kinase C ◽  
Protein Kinase C Inhibitor

The protein kinase C inhibitor CGP 41251, a staurosporine derivative with antitumor activity, reverses multidrug resistance

1994 ◽  
Vol 57 (1) ◽  
pp. 104-110 ◽  
Author(s):  
Irene Utz ◽  
Susanne Hofer ◽  
Urs Regenass ◽  
Wolfgang Hilbe ◽  
Josef Thaler ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Multidrug Resistance ◽  
Protein Kinase ◽  
Antitumor Activity ◽  
Kinase C ◽  
Protein Kinase C Inhibitor

Posttranscriptional regulation of ribonucleotide reductase R1 gene expression is linked to a protein kinase C pathway in mammalian cells

1994 ◽  
Vol 72 (7-8) ◽  
pp. 251-256 ◽  
Author(s):  
Frank Y. Chen ◽  
Francis M. Amara ◽  
Jim A. Wright
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Ribonucleotide Reductase ◽  
Mrna Stability ◽  
Posttranscriptional Regulation ◽  
Signal Pathway ◽  
Kinase C ◽  
Gf 109203X ◽  
Protein Kinase C Inhibitor ◽  
R1 Gene

A rate-limiting reaction in DNA synthesis is catalyzed by ribonucleotide reductase, the enzyme responsible for reducing ribonucleotides to provide the deoxyribonucleotide precursors of DNA. In this study, we have tested the hypothesis that posttranscriptional regulation of ribonucleotide reductase R1 gene expression is controlled by a protein kinase C signal pathway. We show that mouse BALB/c 3T3 fibroblasts treated with the potent and highly specific protein kinase C inhibitor bisindolylmaleimide GF 109203X contain significantly reduced steady-state levels of R1 mRNA and protein. Message half-life studies demonstrate that this is due, at least in part, to a marked decrease in R1 message stability in cells treated with the protein kinase C inhibitor. Furthermore, the protein kinase C signal pathway appears to be specifically involved in this regulation since 8-bromo-cAMP, a modulator of the protein kinase A pathway, had no effect on R1 mRNA levels or stability properties. Cross-linking assays revealed that the binding activity of a R1 mRNA 3′-untranslated region binding protein (R1BP), which was previously shown to be involved in the regulation of R1 mRNA stability, was significantly elevated after treatment of the cells with GF 109203X, in a dose-dependent manner. However, treatment with 8-bromo-cAMP at concentrations up to 2.5 mM did not obviously affect the basic level of the R1BP–RNA interaction. These observations provide a better understanding of the biochemical signals that are critical for the cis–trans interaction-mediated posttranscriptional regulation of ribonucleotide reductase R1 gene expression.Key words: protein kinase C, ribonucleotide reductase, mRNA stability, RNA–protein interaction, bisindolylmaleimide GF 109203X, 8-bromo-cAMP.

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