scholarly journals Effect of transfection of a Drosophila topoisomerase II gene into a human brain tumour cell line intrinsically resistant to etoposide

1996 ◽  
Vol 73 (11) ◽  
pp. 1373-1380 ◽  
Author(s):  
T Asano ◽  
LA Zwelling ◽  
T An ◽  
A McWatters ◽  
CE Herzog ◽  
...  
Keyword(s):  
Human Brain ◽  
Cell Line ◽  
Brain Tumour ◽  
Tumour Cell ◽  
Topoisomerase Ii ◽  
Tumour Cell Line

Antibodies in the sera of patients with bronchogenic carcinoma that react with antigen from a tumour cell line

1981 ◽  
Vol 12 (1) ◽  
pp. 5-10 ◽  
Author(s):  
Barbara S. Kelly ◽  
Ulrike Stredulinsky ◽  
Joan Vanden Hoek ◽  
Julia G. Levy
Keyword(s):  
Cell Line ◽  
Tumour Cell ◽  
Bronchogenic Carcinoma ◽  
Tumour Cell Line

289. Comparison of lymphocyte subsets and function in the rat and mouse testis

2005 ◽  
Vol 17 (9) ◽  
pp. 122 ◽  
Author(s):  
D. Aridi ◽  
D. Pellicci ◽  
P. Hutchinson ◽  
M. P. Hedger
Keyword(s):  
T Cells ◽  
Cell Line ◽  
Tumour Cell ◽  
Flow Cytometric Analysis ◽  
Nkt Cells ◽  
Mouse Testis ◽  
Tumour Cell Line ◽  
Rat Testis ◽  
Flow Cytometric ◽  
Rats And Mice

Testicular leukocytes are assumed to be involved in immunological surveillance against infection and tumours as well as regulation of local immune responses. They are implicated in mechanisms that make the testis a successful site for tissue transplantation in both rats and mice. Our previous studies using multi-colour fluorescence flow cytometric analysis to examine isolated testicular leukocytes in the rat testis have established the existence of a significant population of predominantly CD8+ T cells and a comparable number of lymphocytes expressing natural killer (NK) cell markers (NK and NKT cells). The functional activity of these testicular NK and NKT cells subsequently has been confirmed by a standard flow cytometric cytotoxicity assay using an NK-sensitive tumour cell line (YAC-1) and an NKT-sensitive tumour cell line (U937). Similar analyses of mouse testicular leukocytes have shown a slightly different pattern. The data indicate that mouse testicular lymphocytes comprise T cells, NK cells, and NKT cells, similar to the rat testis. However, while the apparent numerical densities of T cells in rat and mouse testes were similar, the numbers of NK and NKT cells were considerably lower in the mouse. Mouse testicular NKT cells were positive for staining with the tetramer CD1d/αGC, which is used to identify classical NKT cells, whereas rat NKT cells did not stain for this marker. Moreover, the CD8/CD4 T cell ratio in the mouse testis displayed a skewing towards the CD4+ subset. These data highlight the possibility that the immunological environment, and hence the course of immunological events, might be quite different in the testes of the two species. The reasons for these differences are not clear, however they should be taken into account when considering studies of testicular immune processes. Finally, comparative studies of immunological process in the testes of rats and mice may be very informative.

scholarly journals Changes accompanying long term tissue culture of an adenovirus type 12 tumour cell line

1968 ◽  
Vol 22 (4) ◽  
pp. 798-807 ◽  
Author(s):  
G C Schild ◽  
J S Oxford ◽  
C W Potter
Keyword(s):  
Tissue Culture ◽  
Cell Line ◽  
Tumour Cell ◽  
Adenovirus Type ◽  
Tumour Cell Line

Interleukin-1β induces nitric oxide production by a mouse pituitary tumour cell line (AtT20/D16)

1993 ◽  
Vol 138 (3) ◽  
pp. 429-435 ◽  
Author(s):  
K. Ohta ◽  
Y. Hirata ◽  
T. Imai ◽  
F. Marumo
Keyword(s):  
Cell Line ◽  
Tumour Cell ◽  
Pituitary Tumour ◽  
No Synthase ◽  
Tumour Cell Line ◽  
Dependent Manner ◽  
Mouse Pituitary ◽  
Dose Dependent ◽  
Inducible Nos ◽  
Acth Release

ABSTRACT To elucidate whether anterior pituitary cells express the nitric oxide (NO) synthase gene, we studied the synthesis of NO and the expression of NO synthase (NOS) mRNA by a mouse pituitary tumour cell line (AtT20/D16). Interleukin-1β (IL-1β) stimulated production of NO2−/NO3− (NOx) in a time-dependent manner and both NOx and cyclic GMP formation were stimulated in a dose-dependent manner by IL-1β. IL-1β-induced NOx production and intracellular cyclic GMP formation were similarly blocked by an NO synthase inhibitor, NG-monomethyl-l-arginine (LNMMA), whose effect was reversed by l-arginine, but not by d-arginine. Dexamethasone inhibited IL-1β-induced NOx production in a dose-dependent manner. A calmodulin inhibitor (W-7) showed no effect on IL-1β-induced NOx production, whereas cycloheximide and the actinomycin D completely inhibited NOx production. Northern blot analysis using cDNA for mouse macrophage-inducible NOS as a probe revealed the expression of inducible NOS mRNA in the cells only after exposure to IL-1β. Although IL-1β stimulated ACTH release from tumour cells, LNMMA failed to affect ACTH release stimulated by IL-1β. These results demonstrate for the first time that a pituitary tumour cell line (AtT20/D16) possesses cytokine-inducible and Ca2+/calmodulin-independent NOS, although NO may not be involved in ACTH release. Journal of Endocrinology (1993) 138, 429–435

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