scholarly journals Canine transmissible venereal sarcoma: Distribution of T and B lymphocytes in blood, draining lymph nodes and tumours at different stages of growth

1981 ◽  
Vol 44 (4) ◽  
pp. 514-521 ◽  
Author(s):  
J P Chandler ◽  
T J Yang
Keyword(s):  
Lymph Nodes ◽  
B Lymphocytes ◽  
T And B Lymphocytes ◽  
Stages Of Growth ◽  
Draining Lymph Nodes

scholarly journals Inhalation of Ortho-Phthalaldehyde Vapor Causes Respiratory Sensitization in Mice

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Victor J. Johnson ◽  
Jeffrey S. Reynolds ◽  
Wei Wang ◽  
Kara Fluharty ◽  
Berran Yucesoy
Keyword(s):  
Lymph Nodes ◽  
B Lymphocytes ◽  
Case Reports ◽  
Inhalation Exposure ◽  
Cytokine Gene Expression ◽  
Respiratory Problems ◽  
Specific Igg ◽  
High Level ◽  
Lymphocyte Populations ◽  
Draining Lymph Nodes

Ortho-Phthalaldehyde (OPA) has been approved for high-level sterilization of heat-sensitive medical instruments and is increasingly being used as a replacement in the healthcare industry for glutaraldehyde, a known sensitizer. Numerous case reports have been published indicating workers and patients experiencing respiratory problems, anaphylaxis, skin reactivity, and systemic antibody production. Our laboratory previously demonstrated that OPA is a dermal sensitizer in mice. The goal of the present study was to determine if OPA is a respiratory sensitizer following inhalation exposure. Mice were exposed to OPA vapor and airway and lymph nodes were examined for cytokine gene expression and alterations in lymphocyte populations. Inhalation of OPA for 3 days resulted in a concentration-dependent increase in lymphocyte proliferation, mainly B lymphocytes, in the draining lymph nodes. A secondary challenge of mice with OPA resulted in a dramatic increase in the population of B lymphocytes expressing IgE. Expression of Th2 (IL-4, IL-5, and IL-13) and anti/proinflammatory (IL-10, TNFα, and IL-1β) cytokine genes was upregulated in the lymph nodes and the nasal mucosa. Mice exposed to the higher concentrations of OPA-produced OPA-specific IgG1 antibodies indicating systemic sensitization. These findings provide evidence that OPA has the potential to cause respiratory sensitization in mice.

Isolation of MUC1-primed B lymphocytes from tumour-draining lymph nodes by immunomagnetic beads

1999 ◽  
Vol 47 (5) ◽  
pp. 272-277 ◽  
Author(s):  
Claudia Petrarca ◽  
Beniamino Casalino ◽  
Silvia von Mensdorff-Pouilly ◽  
Aurelia Rughetti ◽  
Hassan Rahimi ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
B Lymphocytes ◽  
Immunomagnetic Beads ◽  
Draining Lymph Nodes

Nonspecific acid esterase activity: A criterion for differentation of T and B lymphocytes in mouse lymph nodes

1975 ◽  
Vol 5 (4) ◽  
pp. 270-274 ◽  
Author(s):  
J. Mueller ◽  
G. Brun del Re ◽  
H. Buerki ◽  
H.-U. Keller ◽  
M. W. Hess ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
B Lymphocytes ◽  
Esterase Activity ◽  
T And B Lymphocytes

The entry of T and B lymphocytes into rat popliteal lymph nodes undergoing a graft-versus-host reaction

1983 ◽  
Vol 80 (1) ◽  
pp. 143-150 ◽  
Author(s):  
Wayne G. Kimpton ◽  
David C. Poskitt ◽  
Janet Ruby ◽  
Astrida Petersons ◽  
H.Konrad Muller
Keyword(s):  
Lymph Nodes ◽  
B Lymphocytes ◽  
T And B Lymphocytes ◽  
Graft Versus Host Reaction ◽  
Host Reaction ◽  
Graft Versus Host ◽  
Popliteal Lymph Nodes

scholarly journals Pathomorphology of peripheral organs of immunogenesis in cats with spontaneous feline infectious peritonitis

2018 ◽  
Vol 9 (3) ◽  
pp. 460-468
Author(s):  
M. R. Khalaniia ◽  
G. I. Kotsyumbas ◽  
V. V. Pritsak
Keyword(s):  
Lymph Nodes ◽  
B Lymphocytes ◽  
Congo Red ◽  
Clinical Signs ◽  
T And B Lymphocytes ◽  
Methyl Green ◽  
White Pulp ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Steep Decrease

This article presents the results of pathomorphological research on the spleen and mesenteric lymph nodes of 23 dead cats aged from 3 months to 7 years, which in their lifetimes (according to anamnesis, clinical signs, laboratory-instrumental methods of examination and VetExpert FCoV Ab express test) had been diagnosed with infectious peritonitis. All the animals were domestic. Blood was drawn from the subcutaneous vein of the forelimb of the diseased cats. We determined ESR, morphological parameters of blood and content of hemoglobin. For histological and histochemical examinations, we selected samples of spleen and mesenteric lymph nodes, which were fixated in 10% aqueous solution of neutral formalin, Carnoy’s and Bouin’s solutions. The prepared histological sections were stained using haematoxylin and eosin, Van Gieson’s stain, methyl green-pironin stain (Brashe), PAS-reaction (McManus), alcian blue and Congo red. Hematological parameters during 3 weeks of clinical progression of the disease among the cats demonstrated a decrease in the hemoglobin content and in the number of erythrocytes and leukocytes. Possible decrease in the number of lymphocytes indicated the development of an immune-deficiency state. Also, during the development of disease, the animals had possible increase in ESR, which indicated the development of an inflammatory process in the organism and decrease in the number of thrombocytes, which conditioned development of disseminated intravascular coagulation. The anatomical pathology autopsy showed that in most animals the spleen was diminished in size, the surface of the organ was tuberous, the capsule was wrinkled and mat, the edges were sharpened. The histostructural change was accompanied by a sharp depletion of the lymph nodes and reduction in the number of micro- and macrophages, which indicated the reduction of white pulp, rapid inhibition of the activity of T- and B-lymphocytes, plasmacytic and macrophage reaction and manifested in development of immune-deficient condition of the organism. In this process, the reticular carcass of the lymph nodes saturated with PAS-positive and eosinophilic masses was clearly manifested, which indicated formation of fibrinoid. In the spleen of 5 individuals, during staining using Congo red, we found deposition of amyloid masses both in the intima of the blood vessels and along the reticulary fibers of the lymph nodes. In the cytoplasm of macrophages, we found pyroninophilic formations. In two cases, we observed blood accumulation of red pulp and bleeding following the reduction of white pulp, and in one case fibrinogenous perisplenitis. In the mesenteric lymph nodes of most of the cats which had suffered from infectious peritonitis, we determined that edema, exposure of the reticular soft skeleton (stroma) of adrenal and paracortical zones, dilation of the border and central sinuses and thrombosis of vessels were followed by steep decrease in the number of T- and B-lymphocytes, plasma cells, micro- and macrophages, which indicated the development of atrophic processes of lympoid tissue and immune-suppression. In three cases, in mesenteric lymph nodes of cats, we determined development of sinus histiocytosis. The changes determined in the spleen and lymph nodes of the cats which had suffered from FIP indicate immune-suppressed condition and steep decrease in the functional ability of the organs and organism in general.

scholarly journals Identification of bovine T and B lymphocytes in normal peripheral blood, lymph nodes and spleen.

1983 ◽  
Vol 45 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Hisashi NAKANISHI ◽  
Hiroyuki KOYAMA ◽  
Osamu KAJIKAWA ◽  
Hiroshi SAITO
Keyword(s):  
Lymph Nodes ◽  
B Lymphocytes ◽  
Peripheral Blood ◽  
T And B Lymphocytes ◽  
Normal Peripheral Blood

scholarly journals Inverse expression of bcl-2 protein and Fas antigen in lymphoblasts in peripheral lymph nodes and activated peripheral blood T and B lymphocytes

Blood ◽  
1994 ◽  
Vol 83 (7) ◽  
pp. 1856-1861 ◽  
Author(s):  
T Yoshino ◽  
E Kondo ◽  
L Cao ◽  
K Takahashi ◽  
K Hayashi ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
B Lymphocytes ◽  
Peripheral Blood ◽  
Lymphoid Cells ◽  
Interleukin 4 ◽  
T And B Lymphocytes ◽  
Pokeweed Mitogen ◽  
Fas Antigen ◽  
High Level ◽  
Resting Lymphocytes

Abstract To examine the regulatory mechanism of apoptosis in lymphoid cells, expression of both bcl-2 protein and Fas antigen was investigated in reactive lymph nodes, in resting lymphocytes from peripheral blood (PBLs), and in PBLs stimulated with pokeweed mitogen, interleukin-4 (IL- 4) + anti-IgM antibody, IL-2 + anti-CD3 antibody, phytohemagglutinin + phorbol myristate acetate using immunohistochemistry and flow cytometry. Germinal center cells expressed a large amount of Fas antigen, which is associated with the induction of apoptosis in lymphoid cell lines, in contrast to the lack of bcl-2 protein as an apoptosis inhibitor. On the other hand, mantle zone lymphocytes expressed a high level of bcl-2 protein and less Fas antigen. This inverse expression of bcl-2 protein and Fas antigen was also shown in activated T and B lymphocytes from peripheral blood. These lymphoblasts fell into apoptosis dose-dependently in the presence of anti-Fas monoclonal antibody, but resting lymphocytes that expressed both bcl-2 protein and Fas antigen did not undergo apoptosis. These findings suggest that bcl-2 expression prevents the apoptosis of lymphoid cells induced by the Fas antigen-dependent mechanism and that apoptosis of lymphocytes is exquisitely controlled, at least in part, by regulation of the bcl-2 and Fas genes.

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