scholarly journals Rauscher virus-induced reticulum-cell sarcomas: their growth in vitro and erythropoietic differentiation

1977 ◽  
Vol 35 (2) ◽  
pp. 199-207 ◽  
Author(s):  
A H Fieldsteel ◽  
P J Dawson ◽  
F A Becker ◽  
C Kurahara ◽  
C Mitoma
Keyword(s):  
Reticulum Cell ◽  
Rauscher Virus

scholarly journals Increased Circulating Colony-Stimulating Factor-1 (CSF-1) in SJL/J Mice With Radiation-Induced Acute Myeloid Leukemia (AML) Is Associated With Autocrine Regulation of AML Cells by CSF-1

Blood ◽  
1997 ◽  
Vol 89 (7) ◽  
pp. 2537-2545 ◽  
Author(s):  
Nechama Haran-Ghera ◽  
Rita Krautghamer ◽  
Tsvee Lapidot ◽  
Alpha Peled ◽  
Melissa G. Dominguez ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Mononuclear Phagocyte ◽  
Reticulum Cell ◽  
Colony Stimulating Factor ◽  
Cell Neoplasm ◽  
Radiation Induced ◽  
Stimulating Factor ◽  
Acute Myeloid

Abstract The SJL/J mouse strain has a high spontaneous incidence of a B-cell neoplasm, reticulum cell neoplasm type B (RCN B). In addition, following irradiation, 10% to 30% of these mice develop acute myelomonocytic leukemia (radiation-induced acute myeloid leukemia [RI-AML]), an incidence that can be increased to 50% by treatment of the mice with corticosteroids after irradiation. The role played by the mononuclear phagocyte growth factor, colony-stimulating factor-1 (CSF-1), in the development of RI-AML in SJL/J mice was investigated. Mice dying of RI-AML, but not those dying of RCN B or without disease, possessed elevated concentrations of circulating CSF-1. In addition, in mice developing RI-AML with a more prolonged latency, circulating CSF-1 concentrations were increased before overt expression of RI-AML. First-passage tumors from 14 different RI-AMLs all contained high concentrations of CSF-1, and six of six different first- or second-passage tumors expressed the CSF-1 receptor (CSF-1R). Furthermore, in vitro colony formation by first- or second-passage tumor cells from 20 of 20 different RI-AMLs was blocked by neutralizing anti–CSF-1 antibody, and four of four of these tumors were inhibited by anti–CSF-1R antibody. The results of these antibody neutralization studies, coupled with the observation of elevated circulating CSF-1 in mice developing RI-AML, show an autocrine role for CSF-1 in RI-AML development in SJL/J mice. Southern blot analysis of tumor DNA from six of six of these tumors failed to reveal any rearrangements in the genes for CSF-1 or the CSF-1R. Studies in humans have shown that patients with AML possess elevated levels of circulating CSF-1 and that AML cells can express CSF-1 and the CSF-1R. Thus, RI-AML in the SJL/J mouse appears to be a useful model for human AML.

scholarly journals Expression of hybrid Ia molecules on the cell surface of reticulum cell sarcomas that are undetectable on host SJL/J lymphocytes

1981 ◽  
Vol 153 (3) ◽  
pp. 501-513 ◽  
Author(s):  
SM Wilbur ◽  
B Bonavida
Keyword(s):  
Cell Surface ◽  
Reticulum Cell ◽  
Cell Binding ◽  
Spleen Cells ◽  
Normal Spleen ◽  
Tumor Recognition ◽  
Α Chain ◽  
Chain Synthesis ◽  
Β Chain

SJL/J (H-2 (8)) lymphocytes, primed in vitro against primary, cultured, and transplantable syngeneic reticulum cell sarcomas (RCS) were found to recognize and bind to the tumor without subsequent cytolysis. Additional data showed that the recognition was also directed against Ia molecules of the H-2(d), but not H-2(k), haplotype. Normal spleen cells of DBA/2, B 10.D2, and B 10.OL mice were bound, whereas those of CBA, B 10.BR, B 10.A, B 10.GD, and D2.GD were not. Furthermore, the Ia molecules were in the form of a hybrid, because spleen cells from F(1) progeny of a B10.A and a B10.GD parent were recognized and bound as effectively as the RCS. Recognition was not restricted solely to the H-2(d) haplotype. Spleen cells from B10.S(9R) mice were also significantly bound. This result suggested that the RCS expresses a hybrid Ia molecule containing a β-chain of the H-2(8) haplotype. Recognition of this hybrid Ia molecule by the host resulted in a cross- reactive recognition of H-2(d) specificities. Further analysis revealed that the RCS express on their cell surface an α-chain of the hybrid Ia molecule which is involved in host anti-tumor recognition. Preincubation of the RCS with monoclonal antibody directed against the Ia.7 specificity on the α-chain could block lymphocyte-to-tumor cell binding. The blocking activity could be removed by preabsorption of the antibody on the RCS, as well as normal Ia.7-bearing lymphocytes, but not on lymphocytes that do not express Ia.7, such as SJL/J. The data suggest that the hybrid Ia molecules expressed on the RCS, and recognized by tumor-primed syngeneic lymphocytes, are composed of both a syngeneic and an alien chain. The component alien to the SJL/J host is the Ia.7-bearing α-chain. Normal SJL/J cells synthesize but do not express the β-chain. In the RCS, however, alien α-chain synthesis permits expression of the syngeneic β-chain in the form of a hybrid Ia molecule.

In vitro interference between low and high leukaemogenic variants of rauscher virus

1973 ◽  
Vol 11 (1) ◽  
pp. 224-230
Author(s):  
Rosemary E. Boyd ◽  
Jung Koo Youn ◽  
Georges Barski
Keyword(s):  
Rauscher Virus

Blastic Transformation Induced on the Lymphocytes «in vitro» by an Exudate of Reticulum Cell Sarcoma

1967 ◽  
Vol 53 (5) ◽  
pp. 483-493 ◽  
Author(s):  
Vincenzo Grifoni ◽  
Claudio Rugakli ◽  
Bruna Tossi ◽  
Carlo Besana ◽  
Alessandra Forni
Keyword(s):  
Blood Lymphocyte ◽  
Lymphocyte Culture ◽  
Reticulum Cell ◽  
Reticulum Cell Sarcoma ◽  
Blastic Transformation ◽  
Cell Sarcoma ◽  
Blast Cells ◽  
Sarcoma Patient ◽  
Pleural Exudate

The blood lymphocyte culture «in vitro» has been employed in order to investigate (a) the presence of neoplastic antigens in reticulum cell sarcoma exudates, and (b) the ability of patients with reticulum cell proliferative diseases (reticulum cell sarcoma, reticulum cell reticulosis) to give immune reactions to such antigens. Lymphocytes from a patient with reticulum cell reticulosis showed a 7.3% transformation to blast cells in the presence of the pleural exudate from a reticulum cell sarcoma patient. However, this same exudate did not induce transformation of lymphocytes from two other patients with reticulum cell sarcoma nor of normal lymphocytes. Lymphocytes from the exudate donor patient did show a small percentage transformation in the presence as well as in the absence of the exudate (2.3, and 2.1%, respectively). With the purpose of identification of the transformating factor, fractionation studies were performed yielding a carbohydrate-rich, partially dialyzable mucoprotein. The conclusion is drawn that lymphocytes from the patient with reticulum cell reticulosis were immunologically reactive to a neoplastic antigen contained in the reticulum cell sarcoma exudate, and possibly produced by the proliferating reticulum cells.

Viropexis and Phagocytosis of Type B (Mouse Mammary Tumor) Virus Particles in vivo

1973 ◽  
Vol 31 ◽  
pp. 580-581
Author(s):  
Gabriel Seman ◽  
Leon Dmochowski
Keyword(s):  
Tumor Cells ◽  
Type A ◽  
Reticulum Cell ◽  
Type B ◽  
Cytoplasmic Matrix ◽  
Virus Particles ◽  
Tumor Virus ◽  
Type C

Electron microscope examination of spleen tumors of RIII/Dm female mice with reticulum cell sarcoma revealed the presence of extracellular and budding type C, type B, and intracytoplasmic type A virus particles. Penetration of type B particles into cytoplasm of tumor cells has been observed. It consisted of inclusion of the particles into pinocytotic vesicles and of the release of the nucleoids into the cytoplasmic matrix. They were frequently seen penetrating into tumor cells producing type C or intracytoplasmic type A particles at the same time (Figs. 1 and 2). A similar penetration of other viruses into cells in vitro has been described as viropexis.

Herpestype Virus and Type C Particles in Spontaneous Equine Lymphoma

1970 ◽  
Vol 28 ◽  
pp. 162-163
Author(s):  
K. Maruyama ◽  
G. R. Swearingen ◽  
L. Dmochowski ◽  
J. H. Jardine ◽  
G. N. Joiner
Keyword(s):  
Lymph Node ◽  
Cultured Cells ◽  
Reticulum Cell ◽  
Cell Type ◽  
Virus Particles ◽  
Lymph Node Cells ◽  
Type C ◽  
Indented Nucleus ◽  
Nuclear Protrusion

Herpestype and type C virus particles are known to produce tumors in animals of certain species, and to be present in a variety of human tissues. Similar particles were found in a horse lymphoma.Electron microscope study was carried out on a tumorous prescapular lymph node from an adult female horse with spontaneous lymphoma. Lymph node cells grown in vitro for a total of 105 days (6th passage) were also studied. The lymph node was infiltrated by blastic cells of reticulum cell type and of lymphoblast type. No virus particles were found in the original lymph node. The cultured cells have a large cytoplasm with well developed rough endoplasmic reticulum and Golgi's apparatus, and an indented nucleus with marginated chromatin. Occasionally, intranuclear fibrous structures were observed. Elongated nuclear protrusion were frequently seen.

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