The Contents of Trichloroacetic Acid-soluble Sulphydryl Compounds and Ascorbic Acid in the Liver of Rats Fed Aminoazo Dyes: the Effect of Continuous Feeding of Dyes in the Diet

The ferric reducing anti-oxidant power (FRAP) assay involved the following steps: a) preparation of samples, b) reactions and c) finally measuring absorbance of sample and standard at 700 nm using spectrophotometer. The samples were methanolic extract, different fractions (n-hexane, chloroform) and standard ascorbic acid. The solutions prepared were buffer solution (pH 6.6), 1% potassium ferricyanide solution, 10% trichloroacetic acid and 0.1% ferric chloride. Video Clip<a href="https://www.youtube.com/v/s7BpxNbN5dE">Ferric reducing anti-oxidant power assay:</a>21 min 18 sec

Download Full-text

Vitamin E and stress

British Journal Of Nutrition ◽

10.1079/bjn19670069 ◽

1967 ◽

Vol 21 (3) ◽

pp. 671-679 ◽

Cited By ~ 15

Author(s):

M. A. Cawthorne ◽

A. T. Diplock ◽

I. R. Muthy ◽

J. Bunyan ◽

Elspeth A. Murrell ◽

...

Keyword(s):

Lipid Peroxidation ◽

Ascorbic Acid ◽

Vitamin E ◽

Lipid Peroxide ◽

Toxic Effects ◽

Peroxide Content ◽

In Vitro Techniques ◽

Sulphydryl Compounds

1. Vitamin E-deficient rats were found to be more susceptible than vitamin E-supplemented controls to the toxic effects of hyperbaric oxygen (60 lb/in.2 for 20 min). This agrees with the findings of other workers.2. Hyperbaric O2 treatment did not increase the metabolic destruction of a small amount (46.65 μg) of [14C-5-Me]D-α-tocopherol given to adult vitamin E-deficient rats 24 h previously. The O2 treatment also did not affect the soluble sulphydryl compounds and ascorbic acid of rat liver, nor the percentag haemolysis in vivo of rat blood.3. Hyperbaric O2 treatment did not increase the true lipid peroxide content of rat brain, compared to control rats treated with hyperbaric air, which has no toxic effects. Increases in ‘lipid peroxidation’ reported by previous workers are considered to have been due to the use of inadequate controls (untreated rats) and of in vitro techniques that are open to criticism.4. The toxic effects of hyperbaric O2 in the vitamin E-deficient rat cannot be attributed to peroxidation in vivo.5. Vitamin E was not found to protect rats against the effects of reduced O2 tension (anoxic anoxia). This finding contrasts with some reports by earlier workers. Reduced O2 tension had no effect on the metabolism of radioactive tocopherol, on blood haemolysis in vivo, or on the soluble sulphydryl compounds and ascorbic acid of liver.

Download Full-text

Use of p-Chloromercuribenzoic Acid in the Determination of Ascorbic Acid in the Presence of Sulphydryl Compounds

Nature ◽

10.1038/174701a0 ◽

1954 ◽

Vol 174 (4432) ◽

pp. 701-701 ◽

Cited By ~ 7

Author(s):

J. A. OWEN ◽

B. IGGO ◽

D. B. HORN

Keyword(s):

Ascorbic Acid ◽

Sulphydryl Compounds

Download Full-text

Comparison of Ascorbic Acid Concentrations in Whole Blood Obtained by Venipuncture or by Finger Prick

Clinical Chemistry ◽

10.1093/clinchem/18.9.968 ◽

1972 ◽

Vol 18 (9) ◽

pp. 968-970 ◽

Cited By ~ 9

Author(s):

Daniel W Bradley ◽

James E Maynard ◽

Gladys Emery

Keyword(s):

Ascorbic Acid ◽

Vitamin C ◽

Whole Blood ◽

Trichloroacetic Acid ◽

The Third ◽

C Storage ◽

Finger Prick ◽

Additional Losses

Abstract Vitamin C (ascorbic acid) concentrations in whole blood obtained by finger prick or by venipuncture have been shown to be equivalent when measured by the 2,4-dinitrophenylhydrazine procedure. Whole blood obtained by either technique is stable for at least 6 h at 25°C, and for as long as 28 days when stabilized with trichloroacetic acid and stored at 4°C. Storage of whole blood at -70°C is accompanied by an initial loss (10-20%) of vitamin C; however, additional losses of vitamin C do not occur beyond the third day of storage.

Download Full-text

Combined trichloroacetic acid peel and topical ascorbic acid versus trichloroacetic acid peel alone in the treatment of melasma: a comparative study

Journal of Cosmetic Dermatology ◽

10.1111/j.1473-2165.2007.00302.x ◽

2007 ◽

Vol 6 (2) ◽

pp. 89-94 ◽

Cited By ~ 26

Author(s):

Mohsen Mohamed Soliman ◽

Shahira Abdel-Rahman Ramadan ◽

Dalia Ahmed Bassiouny ◽

Marwa Abdelmalek, MSc

Keyword(s):

Ascorbic Acid ◽

Comparative Study ◽

Trichloroacetic Acid ◽

Acid Versus

Download Full-text

Advantages of Use of Ascorbic Acid and Sodium Lauryl Sulfate in the Microdetermination of Serum the Inorganic Phosphorus

Clinical Chemistry ◽

10.1093/clinchem/28.4.708 ◽

1982 ◽

Vol 28 (4) ◽

pp. 708-708

Author(s):

J B Bingley ◽

M D G T de Postiglione

Keyword(s):

Ascorbic Acid ◽

Sulfuric Acid ◽

Trichloroacetic Acid ◽

Inorganic Phosphorus ◽

Ammonium Molybdate ◽

Distilled Water ◽

Lauryl Sulfate ◽

The Third ◽

Exact Degree ◽

Middle Column

Abstract Vol. 27 p 639: In the second paragraph of the middle column, the third sentence (et seq.) should read as follows: In the method of Chen et al. (12), modified to include SLS 1,040 mmol/L, 25 µL of serum was added to 25 µL of distilled water. To this was added 200 µL of a solution of SLS containing 1040 mmol/L, followed, after mixing, by 2.50 mL of a reagent consisting of 3.1 g of ammonium molybdate per liter of 375 mmol/L sulfuric acid. To avoid any instability in the reducing capacity of ascorbic acid if mixed in advance with molybdate and sulfuric acid (14), we added it separately—0.200 mL of a 150 g/L solution in glass-distilled water. After incubation in a warm bath at 37 °C for 140 min (12), the replicated stoppered samples were cooled and their absorbances (820 nm, 1-cm cells) read immediately and 3 and 18 hlater. In those cases where SLS was not used—i.e., in the unmodified method (12)—50 µL of a prepared trichloroacetic acid extract, equivalent to 25 µL of serum, was analyzed in the same manner; 200 µL of distilled water then replaced the SLS. p 1401: In footnote 1, the compound should read N1-(α,α-dimethyl - β - hydroxyethyl) - 2- methyl - 1,2- propanediamine. p 1749: In Table 3, column 4, row 2: "0-20" should read "0-2.0." p 1948: In ref. 12, "Sud-" should read "Sul-" (publication is in Portugese, not Spanish). p 1952: The names of two of the authors are misspelled: Cassani and Vanzetti. The correct spellings in the address are "Granda" and "Niguarda." Vol. 28 p 156: In Table 1, last column, delete "x" from the equations "y = 0.75x" and "y = 1.71x." p 401: In the address in the middle column, "State" should read "Safe." p 406: Third column, under "Natelson Award," Normal should read Norman. p 557: Just before the last sentence in the second paragraph in the middle column, insert the following sentence: "The exact degree of the enhancement depends highly on the amount of catalase impurity in the glucose oxidase preparation."

Download Full-text