scholarly journals Cytotoxic efficacy of filanesib and melphalan combination is governed by sequence of treatment in human myeloma cells

2016 ◽  
Vol 6 (10) ◽  
pp. e480-e480 ◽  
Author(s):  
E J Norris ◽  
D DeStephanis ◽  
B Tunquist ◽  
S Usmani ◽  
R Ganapathi ◽  
...  
Keyword(s):  
Myeloma Cells ◽  
Human Myeloma Cells

Effects of an HMG-CoA reductase inhibitor, simvastatin, on human myeloma cells

2004 ◽  
Author(s):  
Takemi Otsuki ◽  
Haruko Sakaguchi ◽  
Tamayo Hatayama ◽  
Tomohiro Fujii ◽  
Takayuki Tsujioka ◽  
...  
Keyword(s):  
Reductase Inhibitor ◽  
Hmg Coa Reductase ◽  
Myeloma Cells ◽  
Hmg Coa Reductase Inhibitor ◽  
Coa Reductase ◽  
Human Myeloma Cells

scholarly journals Identification of immature and mature myeloma cells in the bone marrow of human myelomas

Blood ◽  
1993 ◽  
Vol 82 (2) ◽  
pp. 564-570 ◽  
Author(s):  
MM Kawano ◽  
N Huang ◽  
H Harada ◽  
Y Harada ◽  
A Sakai ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Adhesion Molecules ◽  
Proliferative Activity ◽  
M Protein ◽  
Color Analysis ◽  
Myeloma Cells ◽  
A Cell ◽  
Immature Cells ◽  
Human Myeloma Cells

Abstract With regard to the expression of adhesion molecules, human myeloma cells freshly isolated from bone marrow were heterogeneous. By two- color analysis with anti-VLA-5 antibody (PE staining) and FITC-labeled anti-CD38 antibody, we found all myeloma cells located at CD38-strong positive (CD38++) fraction and identified two subpopulations among these myeloma cells: CD38++ VLA-5-(VLA-5-) myeloma cells and CD38++ VLA- 5+ (VLA-5+) myeloma cells. To clarify the biologic character of these two subpopulations, the morphology, in vitro proliferative activity and in vitro M-protein secretion were examined in each fraction isolated by the purification procedure or a cell sorter. Morphologic examination showed that VLA-5- myeloma cells were mostly immature or plasmablastic and VLA-5+ cells were mature myeloma cells. Furthermore, VLA-5- myeloma cells proliferated markedly in vitro and responded to interleukin 6 (IL- 6), a growth factor for myeloma cells, while VLA-5+ myeloma cells showed very low uptakes of 3H-thymidine and no responses to IL-6 but secreted higher amounts of M-protein (immunoglobulin) in vitro significantly. Therefore, we could clarify here heterogeneity of human myeloma cells in the bone marrow with regard to the expression of VLA- 5, one of integrin adhesion molecules; VLA-5- myeloma cells were proliferative immature cells and VLA-5+ cells were mature myeloma cells.

Effect of interleukin-2 on the ex vivo growth of human myeloma cells

1989 ◽  
Vol 30 (4) ◽  
pp. 227-232 ◽  
Author(s):  
Dietrich Peest ◽  
Ina de Vries ◽  
Regina Hölscher ◽  
Regine Leo ◽  
Helmuth Deicher
Keyword(s):  
Ex Vivo ◽  
Interleukin 2 ◽  
Myeloma Cells ◽  
Human Myeloma Cells

An IL-6 receptor antagonist for IL-6 activity on human myeloma cells

Cytokine ◽  
1994 ◽  
Vol 6 (5) ◽  
pp. 564
Author(s):  
J.P.J. Brakenhoff ◽  
F.D. de Hon ◽  
M. Ehlers ◽  
J. Grötzinger ◽  
C. Clement ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Myeloma Cells ◽  
Human Myeloma Cells

scholarly journals Percutaneous Exposures of volunteers to polychromatic light (480-3400 nm) trigger systemic mechanism of the human myeloma cells growth delay without any effect on bortezomib cytotoxicity in vitro

LASER THERAPY ◽  
2019 ◽  
Vol 28 (3) ◽  
pp. 164-170
Author(s):  
Natalia V. Kalmykova ◽  
Anna V. Shcherbanyuk ◽  
Sergei I. Moiseev ◽  
Natalia V. Bichkova ◽  
Natalia I. Davidova ◽  
...  
Keyword(s):  
Growth Delay ◽  
Myeloma Cells ◽  
Systemic Mechanism ◽  
Polychromatic Light ◽  
Human Myeloma Cells

Studies on the Clonogenicity of Human Myeloma Cells in vitro

1990 ◽  
Vol 2 (5) ◽  
pp. 271-277
Author(s):  
B. C. Millar ◽  
J. A. Maitland ◽  
J. B. G. Bell ◽  
T. J. McElwain
Keyword(s):  
Myeloma Cells ◽  
Human Myeloma Cells

scholarly journals Sensitive inhibitory effect of interferon-alpha on M-protein secretion of human myeloma cells

Blood ◽  
1989 ◽  
Vol 74 (5) ◽  
pp. 1718-1722 ◽  
Author(s):  
H Tanaka ◽  
O Tanabe ◽  
K Iwato ◽  
H Asaoku ◽  
H Ishikawa ◽  
...  
Keyword(s):  
Interferon Alpha ◽  
Protein Secretion ◽  
Inhibitory Effect ◽  
M Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
In Vitro Proliferation ◽  
Myeloma Cells ◽  
Ifn Alpha ◽  
Human Myeloma Cells

Abstract The effects of interferon-alpha (IFN alpha) on in vitro proliferation and M-protein secretion in human myeloma cells were investigated. Human myeloma cells were purified from bone marrow aspirates in 12 multiple myeloma patients. Purified myeloma cells were cultured for 48 hours with IFN alpha at its lower concentrations (0.1 to 100 U/mL). The cells were then pulsed with 3H-TdR for the last 12 hours and 3H-TdR uptake was measured (in vitro proliferation). After 48-hour culture, supernatants were harvested and the amount of M-protein in these fluids were measured by enzyme-linked immunosorbent assay (ELISA) (in vitro M- protein secretion). In vitro M-protein secretions of myeloma cells were significantly suppressed even at 0.1 U/mL of IFN alpha, while 3H-TdR uptakes were not so suppressed until 10 or 100 U/mL of IFN alpha were added. The expressions of secretory immunoglobulin (Ig) mRNA of these myeloma cells were also selectively suppressed by IFN alpha. Furthermore, after IFN alpha had been administered intramuscularly, 3 to 6 x 10(6) U/d for at least 1 month, in vitro M-protein secretions of these myeloma cells were decreased compared with those before IFN alpha administration. Therefore, these results suggest that IFN alpha has more sensitive inhibitory effect on M-protein secretion of human myeloma cells rather than on myeloma cell proliferation.

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