scholarly journals Characterization of naturally occurring pentacyclic triterpenes as novel inhibitors of deubiquitinating protease USP7 with anticancer activity in vitro

2017 ◽  
Vol 39 (3) ◽  
pp. 492-498 ◽  
Author(s):  
Bo Jing ◽  
Meng Liu ◽  
Li Yang ◽  
Hai-yan Cai ◽  
Jie-bo Chen ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Pentacyclic Triterpenes ◽  
Naturally Occurring ◽  
Deubiquitinating Protease

Functional and Biochemical Characterization of Immunologically Derived Equine Platelet-Activating Factor

1985 ◽  
Vol 22 (4) ◽  
pp. 375-386 ◽  
Author(s):  
H. C. Wimberly ◽  
D. O. Slauson ◽  
N. R. Neilsen
Keyword(s):  
Functional Activity ◽  
Biochemical Characterization ◽  
Platelet Activating Factor ◽  
Biochemical Properties ◽  
Naturally Occurring ◽  
Rabbit Platelets ◽  
Rapid Reaction ◽  
Specific Challenge

Antigen-specific challenge of equine leukocytes induced the non-lytic release of a platelet-activating factor in vitro. The equine platelet-activating factor stimulated the release of serotonin from equine platelets in a dose-responsive manner, independent of the presence of cyclo-oxygenase pathway inhibitors such as indomethacin. Rabbit platelets were also responsive to equine platelet-activating factor. The release of equine platelet-activating factor was a rapid reaction with near maximal secretion taking place in 30 seconds. Addition of equine platelet-activating factor to washed equine platelets stimulated platelet aggregation which could not be inhibited by the presence of aspirin or indomethacin. Platelets preincubated with equine platelet-activating factor became specifically desensitized to equine platelet-activating factor while remaining responsive to other platelet stimuli such as collagen and epinephrine. The following biochemical properties of equine platelet-activating factor are identical to those properties of 1-0-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (AGEPC): stability upon exposure to air and acid; loss of functional activity after basecatalyzed methanolysis with subsequent acylation that returned all functional activity; and identical relative mobilities on silica gel G plates developed with chloroform:methanol:water (65:35:6, volume/volume). The combined functional and biochemical characteristics of equine platelet-activating factor strongly suggest identity between this naturally occurring, immunologically derived equine factor and AGEPC.

Differential Regulation of Articular Cartilage Biomechanical and Biochemical Properties by IGF-1 and TGF-β1 During In Vitro Growth

2009 ◽  
Author(s):  
Michael E. Stender ◽  
Christian R. Flores ◽  
Kristin J. Dills ◽  
Gregory M. Williams ◽  
Kevin M. Stewart ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Growth Models ◽  
Biochemical Properties ◽  
Low Friction ◽  
Cartilage Growth ◽  
Detailed Characterization ◽  
Naturally Occurring ◽  
Tgf Β1

Articular cartilage (AC) is a load bearing material that provides a low friction wear resistant interface in synovial joints. Naturally-occurring and stimulated intrinsic repair of damaged AC is ineffective. Thus, there is a desire to engineer effective replacement tissue that could be used for AC repair. Previous studies [1] have shown that culture of immature cartilage with medium including TGF-β1 will result in a more mature tissue than culture with IGF-1. Detailed characterization of tissue mechanical properties would be helpful for development of cartilage growth models [2].

scholarly journals Isolation, Synthesis, and Antimicrobial Activities of Naturally Occurring θ-Defensin Isoforms from Baboon Leukocytes

2008 ◽  
Vol 76 (12) ◽  
pp. 5883-5891 ◽  
Author(s):  
Angie E. Garcia ◽  
George Ösapay ◽  
Patti A. Tran ◽  
Jun Yuan ◽  
Michael E. Selsted
Keyword(s):  
Bone Marrow ◽  
Single Species ◽  
Antimicrobial Activities ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Naturally Occurring ◽  
Test Organisms ◽  
High Degree

ABSTRACT θ-Defensins are macrocyclic antimicrobial peptides that were previously isolated from leukocytes of a single species, the rhesus macaque. We now report the characterization of baboon θ-defensins (BTDs) expressed in bone marrow and peripheral blood leukocytes. Four cDNAs encoding θ-defensin precursors were characterized, allowing for the prediction of 10 theoretical θ-defensins (BTD-1 to BTD-10) produced by binary, head-to-tail splicing of nonapeptides excised from paired precursors. Five of the predicted θ-defensins were purified from baboon leukocytes, and synthetic versions of each were prepared. Anti-θ-defensin antibody localized the peptides in circulating neutrophils and monocytes and in immature and mature myeloid elements in bone marrow. Each of the BTDs possessed antimicrobial activity against bacterial and fungal test organisms in vitro. Peptide activities varied markedly despite a high degree of sequence conservation among the θ-defensins tested. Thus, baboons express numerous θ-defensins which appear to differentially contribute to host defense against diverse pathogens.

scholarly journals In Vitro Anticancer Activity and Structural Characterization of Ubiquinones from Antrodia cinnamomea Mycelium

Molecules ◽  
2017 ◽  
Vol 22 (5) ◽  
pp. 747 ◽  
Author(s):  
I-Chuan Yen ◽  
Shih-Yu Lee ◽  
Kuen-Tze Lin ◽  
Feng-Yi Lai ◽  
Mao-Tien Kuo ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Structural Characterization ◽  
Antrodia Cinnamomea

scholarly journals Purification and Characterization of a Lectin from Arisaema tortuosum Schott Having in-vitro Anticancer Activity against Human Cancer Cell Lines

BMB Reports ◽  
2005 ◽  
Vol 38 (5) ◽  
pp. 526-532 ◽  
Author(s):  
Vikram Dhuna ◽  
Jagmohan Singh Bains ◽  
Sukhdev Singh Kamboj ◽  
Jatinder Singh ◽  
Shanmugavel ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Purification And Characterization ◽  
Human Cancer Cell Lines

Preparation and Characterization of Genipin-Crosslinked Chitosan Microspheres for the Sustained Release of Salidroside

2015 ◽  
Vol 11 (3) ◽  
pp. 323-333 ◽  
Author(s):  
Mei Luo ◽  
Hailong Peng ◽  
Zhonghua Deng ◽  
Zhonghua Yin ◽  
Qiang Zhao ◽  
...  
Keyword(s):  
Sustained Release ◽  
Functional Foods ◽  
Anomalous Behavior ◽  
Fickian Diffusion ◽  
Release Behavior ◽  
Chitosan Microspheres ◽  
Naturally Occurring ◽  
The Stability

Abstract Chitosan microspheres (CsMs) that encapsulate salidroside (Sal) were prepared by the emulsion crosslinking method with naturally occurring genipin (Gp) and then examined for their in vitro release. Sal-loaded CsMs (Sal-CsMs) showed nearly spherical and smooth surfaces with internal voids. The particle size of Sal-CsMs ranged within 0.56–5.01 μm, and their encapsulation efficiency and loading capacity were beyond 77.58% and 23.29%, respectively. The stability of Sal improved after entrapment into the CsMs. The release rate of Sal from CsMs was initially rapid, followed by sustained release. The release behavior depended on the pH of the release medium. The main release mechanisms underlying the release procedure were anomalous behavior and Fickian diffusion. These results indicated that CsMs with a novel crosslinker of Gp was a potential carrier system for producing functional foods containing Sal.

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