scholarly journals γ-Secretase inhibitor DAPT sensitizes t-AUCB-induced apoptosis of human glioblastoma cells in vitro via blocking the p38 MAPK/MAPKAPK2/Hsp27 pathway

2014 ◽  
Vol 35 (6) ◽  
pp. 825-831 ◽  
Author(s):  
Jun-yang Li ◽  
Ru-jun Li ◽  
Han-dong Wang
Keyword(s):  
P38 Mapk ◽  
Glioblastoma Cells ◽  
Human Glioblastoma ◽  
Secretase Inhibitor ◽  
Human Glioblastoma Cells ◽  
Induced Apoptosis

scholarly journals A New Pathway Promotes Adaptation of Human Glioblastoma Cells to Glucose Starvation

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1249
Author(s):  
Alberto Azzalin ◽  
Francesca Brambilla ◽  
Eloisa Arbustini ◽  
Katia Basello ◽  
Attilio Speciani ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Membrane Trafficking ◽  
Adaptor Protein ◽  
Glucose Starvation ◽  
Glioblastoma Cells ◽  
Glucose Addition ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells ◽  
Inducible Protein

Adaptation of glioblastoma to caloric restriction induces compensatory changes in tumor metabolism that are incompletely known. Here we show that in human glioblastoma cells maintained in exhausted medium, SHC adaptor protein 3 (SHC3) increases due to down-regulation of SHC3 protein degradation. This effect is reversed by glucose addition and is not present in normal astrocytes. Increased SHC3 levels are associated to increased glucose uptake mediated by changes in membrane trafficking of glucose transporters of the solute carrier 2A superfamily (GLUT/SLC2A). We found that the effects on vesicle trafficking are mediated by SHC3 interactions with adaptor protein complex 1 and 2 (AP), BMP-2-inducible protein kinase and a fraction of poly ADP-ribose polymerase 1 (PARP1) associated to vesicles containing GLUT/SLC2As. In glioblastoma cells, PARP1 inhibitor veliparib mimics glucose starvation in enhancing glucose uptake. Furthermore, cytosol extracted from glioblastoma cells inhibits PARP1 enzymatic activity in vitro while immunodepletion of SHC3 from the cytosol significantly relieves this inhibition. The identification of a new pathway controlling glucose uptake in high grade gliomas represents an opportunity for repositioning existing drugs and designing new ones.

scholarly journals Synthesis and anti-glioblastoma effects of artemisinin-isothiocyanate derivatives

RSC Advances ◽  
2018 ◽  
Vol 8 (71) ◽  
pp. 40974-40983
Author(s):  
Chan Myae Nyein ◽  
Xiaolin Zhong ◽  
Junfeng Lu ◽  
Huijuan Luo ◽  
Jiamin Wang ◽  
...  
Keyword(s):  
Glioblastoma Cells ◽  
Cytotoxic Effects ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells ◽  
Induced Apoptosis ◽  
U87 Cells

Synthesis of artemisinin-isothiocyanate derivatives; evaluation of the cytotoxic effects of these compounds on U87 human glioblastoma cells; compound5binduced apoptosis and autophagy in U87 cells; compound5bsignificantly inhibited the migration of U87 cells.

An in vitro study on the antitumor effect of sonodynamic therapy using sinoporphyrin sodium on human glioblastoma cells

Ultrasonics ◽  
2021 ◽  
Vol 110 ◽  
pp. 106272
Author(s):  
Yuanyuan Shen ◽  
Yiling Chen ◽  
Yongpeng Huang ◽  
Xiaojun Zeng ◽  
Lanhui Huang ◽  
...  
Keyword(s):  
Antitumor Effect ◽  
In Vitro Study ◽  
Vitro Study ◽  
Glioblastoma Cells ◽  
Sonodynamic Therapy ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells

Down-regulation of ribosomal protein S15A inhibits proliferation of human glioblastoma cells in vivo and in vitro via AKT pathway

Tumor Biology ◽  
2015 ◽  
Vol 37 (4) ◽  
pp. 4979-4990 ◽  
Author(s):  
Yiqun Yao ◽  
Yongjian Liu ◽  
Xiupeng Lv ◽  
Bin Dong ◽  
Feng Wang ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Akt Pathway ◽  
Glioblastoma Cells ◽  
Down Regulation ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells

scholarly journals Effects of X-irradiation Alone and in Combination with ACNU on Human Glioblastoma Cells in Vitro

1990 ◽  
Vol 30 (5) ◽  
pp. 295-300 ◽  
Author(s):  
Shoji MASHIYAMA ◽  
Ryuichi KATAKURA ◽  
Kou TAKAHASHI ◽  
Masakazu KITAHARA ◽  
Jiro SUZUKI ◽  
...  
Keyword(s):  
Glioblastoma Cells ◽  
Human Glioblastoma ◽  
X Irradiation ◽  
Human Glioblastoma Cells

Icaritin Sensitizes Human Glioblastoma Cells to TRAIL-Induced Apoptosis

2015 ◽  
Vol 72 (2) ◽  
pp. 533-542 ◽  
Author(s):  
Hongxing Han ◽  
Bo Xu ◽  
Pengzhi Hou ◽  
Chuanwu Jiang ◽  
Longxi Liu ◽  
...  
Keyword(s):  
Glioblastoma Cells ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells ◽  
Induced Apoptosis

scholarly journals CYTOTOXICITY AND HEMOCOMPATIBILITY OF DOXORUBICIN-LOADED PLGA NANOPARTICLES

2020 ◽  
Vol 19 (1) ◽  
pp. 71-80
Author(s):  
Yu. A. Malinovskaya ◽  
E. I. Kovalenko ◽  
T. S. Kovshova ◽  
N. S. Osipova ◽  
O. O. Maksimenko ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Blood Coagulation ◽  
Hemoglobin Concentration ◽  
Coagulation Cascade ◽  
Coagulation System ◽  
Intracranial Tumour ◽  
Glioblastoma Cells ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells

Introduction. The use of polymeric biodegradable nanoparticles (NP) as drug delivery systems is a promising approach to overcome histohematomatic barriers. Thus, poloxamer 188-coated poly (lactide-co-glycolide) (PLGA) NP are able to overcome blood-brain barrier and to deliver therapeutic agents, in particular doxorubicin, into intracranial tumour upon intravenous administration. It is important to evaluate NP interaction with blood components in preclinical studies.The objective of the study was to investigate cytotoxicity and hemocompatibility of doxorubicin-loaded PLGA NP (Dox-PLGA NP), to essess NP uptake by glioblastoma cells.Materials and methods. The influence of NP on coagulation cascade was evaluated by prothrombin time measuring before and after plasma incubation with NP. To assess NP thrombogenicity the platelet activation level was determined by flow cytometry. The NP hemolytic activity (released hemoglobin concentration) was measured spectrophotometrically. NP cytotoxicity was determined by MTS assay. NP uptake by human glioblastoma cells was evaluated by flow cytometry.Results. Dox-PLGA NP did not influence blood coagulation time and thrombocyte activity at concentrations up to 100 mcg/mL: PT values were 12–15 s for all tested samples, and P-selectin expression level did not exceed 15 %. All samples were not hemolytic after 3 h of incubation. Cytotoxicity of doxorubicin released from PLGA NP on glioma U87MG cells was comparable to that of free doxorubicin. As shown by flow cytometry Dox-PLGA NP were efficiently internalized into the cells.Conclusion. The study of hemocompatibility confirmed the safety of Dox-PLGA NP: NP did not influence blood coagulation system and did not induce hemolysis. NP were efficiently internalized into the human glioblastoma cells and produced considerable antitumor effect in vitro.

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