scholarly journals One drop at a time: toward droplet microfluidics as a versatile tool for single-cell analysis

2014 ◽  
Vol 6 (10) ◽  
pp. e133-e133 ◽  
Author(s):  
Agata Rakszewska ◽  
Jurjen Tel ◽  
Venkatachalam Chokkalingam ◽  
Wilhelm TS Huck
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Droplet Microfluidics ◽  
Cell Analysis ◽  
Versatile Tool

scholarly journals Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis

Molecules ◽  
2016 ◽  
Vol 21 (7) ◽  
pp. 881 ◽  
Author(s):  
Na Wen ◽  
Zhan Zhao ◽  
Beiyuan Fan ◽  
Deyong Chen ◽  
Dong Men ◽  
...  
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Single Cell Analysis ◽  
Droplet Microfluidics ◽  
Cell Analysis

scholarly journals Droplet Microfluidics: Single‐Cell Analysis Using Droplet Microfluidics (Adv. Biosys. 1/2020)

2020 ◽  
Vol 4 (1) ◽  
pp. 2070012
Author(s):  
Kinga Matuła ◽  
Francesca Rivello ◽  
Wilhelm T. S. Huck
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Droplet Microfluidics ◽  
Cell Analysis

scholarly journals Optimized double emulsion flow cytometry with high-throughput single droplet isolation

2019 ◽  
Author(s):  
Kara K. Brower ◽  
Catherine Carswell-Crumpton ◽  
Sandy Klemm ◽  
Bianca Cruz ◽  
Gaeun Kim ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
High Throughput ◽  
Single Cell Analysis ◽  
Double Emulsion ◽  
Droplet Microfluidics ◽  
Cell Analysis ◽  
Single Droplet ◽  
Emulsion Droplets ◽  
Droplet Sorting

Droplet microfluidics has made large impacts in diverse areas such as enzyme evolution, chemical product screening, polymer engineering, and single-cell analysis. However, while droplet reactions have become increasingly sophisticated, phenotyping droplets by a fluorescent signal and sorting them to isolate variants-of-interest remains a field-wide bottleneck. Here, we present an optimized double emulsion workflow, sdDE-FACS, that enables high-throughput phenotyping, selection, and sorting of droplets using standard flow cytometers. Using a 130 μm nozzle, we demonstrate robust post-sort recovery of intact droplets, with little to no shear-induced droplet breakage, at high sort frequency (12-14 kHz) across two industry-standard FACS instruments. We report the first quantitative plate statistics for double emulsion droplet isolation and demonstrate single droplet recovery with >70% efficiency. In addition, we establish complete downstream recovery of nucleic acids from single, sorted double emulsion droplets, an advance in droplet sorting comparable with the capabilities of single-cell FACS. This work resolves several hurdles in the field of high-throughput droplet analysis and paves the way for a variety of new droplet assays, including rare variant isolation and multiparameter single-cell analysis, marrying the full power of flow cytometry with droplet microfluidics.

scholarly journals Single‐Cell Analysis Using Droplet Microfluidics

2019 ◽  
Vol 4 (1) ◽  
pp. 1900188 ◽  
Author(s):  
Kinga Matuła ◽  
Francesca Rivello ◽  
Wilhelm T. S. Huck
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Droplet Microfluidics ◽  
Cell Analysis

ChemInform Abstract: Droplet Microfluidics - A Tool for Single-Cell Analysis

ChemInform ◽  
2013 ◽  
Vol 44 (21) ◽  
pp. no-no
Author(s):  
Haakan N. Joensson ◽  
Helene Andersson Svahn
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Droplet Microfluidics ◽  
Cell Analysis
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