scholarly journals Diagnostic Utility of Major Basic Protein, Eotaxin-3, and Leukotriene Enzyme Staining in Eosinophilic Esophagitis

2012 ◽  
Vol 107 (10) ◽  
pp. 1503-1511 ◽  
Author(s):  
Evan S Dellon ◽  
Xiaoxin Chen ◽  
Ryan C Miller ◽  
John T Woosley ◽  
Nicholas J Shaheen
Keyword(s):  
Eosinophilic Esophagitis ◽  
Basic Protein ◽  
Diagnostic Utility ◽  
Major Basic Protein

Eosinophil derived major basic protein deposition occurs in idiopathic eosinophilic esophagitis

1998 ◽  
Vol 114 ◽  
pp. A126-A127 ◽  
Author(s):  
GT Furuta ◽  
S Walsh ◽  
DA Antonioli ◽  
MY Wang ◽  
SJ Ackerman ◽  
...  
Keyword(s):  
Eosinophilic Esophagitis ◽  
Basic Protein ◽  
Major Basic Protein ◽  
Protein Deposition

scholarly journals Utility of major basic protein, eotaxin-3, and mast cell tryptase staining for prediction of response to topical steroid treatment in eosinophilic esophagitis: analysis of a randomized, double-blind, double dummy clinical trial

2020 ◽  
Vol 33 (6) ◽  
Author(s):  
Evan S Dellon ◽  
John T Woosley ◽  
Sarah J McGee ◽  
Susan E Moist ◽  
Nicholas J Shaheen
Keyword(s):  
Mast Cell ◽  
Randomized Trial ◽  
Eosinophilic Esophagitis ◽  
Basic Protein ◽  
Topical Steroid ◽  
Inflammatory Factors ◽  
Topical Steroids ◽  
Major Basic Protein ◽  
Mast Cell Tryptase ◽  
Histologic Response

Summary Inflammatory factors in eosinophilic esophagitis (EoE), including major basic protein (MBP), eotaxin-3 (EOT3) and mast cell tryptase (TRP), may predict treatment response to topical corticosteroids (tCS). We aimed to determine whether baseline levels of these markers predict response to tCS for EoE. To do this, we analyzed data from a randomized trial comparing two topical steroids for treatment of newly diagnosed EoE (NCT02019758). A pretreatment esophageal biopsy was stained for MBP, EOT3, and TRP to quantify tissue biomarker levels (cells/mm2). Levels were compared between histologic responders (<15 eos/hpf) and nonresponders (the primary outcome), and endoscopic responders (EREFS<2) and nonresponders. Complete histologic response (<1 eos/hpf) was also assessed, and area under the receiver operator characteristic curve (AUC) was calculated. We also evaluated whether baseline staining predicted symptom relapse in the trial’s off-treatment observation phase. Baseline samples were evaluable in 110/111 subjects who completed the randomized trial. MBP levels were higher in nonresponders (n = 36) than responders (704 vs. 373 cells/mm2; P = 0.007), but EOT3 and TRP levels were not statistically different. The combination of all three stains had an AUC of 0.66 to predict response. For complete histologic response, baseline TRP levels were higher in nonresponders (n = 69) than responders (370 vs. 268 mast cells/mm2; P = 0.01), with an AUC of 0.65. The AUC for endoscopic response was 0.68. Baseline staining did not predict symptom recurrence after remission. Pretreatment MBP, EOT3, and TRP levels were not strongly or consistently associated with histologic or endoscopic response to topical steroids. While elevated TRP levels may be associated with nonresponse compared with complete response, the magnitude and predictive utilities were modest. Novel methods for predicting steroid response are still required.

scholarly journals Eosinophil granule major basic protein 1 deposition in eosinophilic esophagitis correlates with symptoms independent of eosinophil counts

2019 ◽  
Author(s):  
K A Peterson ◽  
G J Gleich ◽  
N S Limaye ◽  
H Crispin ◽  
J Robson ◽  
...  
Keyword(s):  
Eosinophilic Esophagitis ◽  
Basic Protein ◽  
Histopathological Examination ◽  
Major Basic Protein ◽  
Esophageal Biopsy ◽  
Protein Deposition ◽  
Granule Protein ◽  
Biopsy Specimens ◽  
Eosinophil Granule ◽  
Eosinophil Counts

SUMMARY In patients with eosinophilic esophagitis (EoE), symptoms often do not correlate with peak eosinophil counts (PEC) determined on histopathological examination of biopsy specimens. This may be because eosinophils degranulate during active disease and lose their morphological identity as intact cells and, therefore, are not enumerated on microscopic examination. Eosinophil granule proteins that are released into tissues with degranulation, including major basic protein 1 (eMBP1), likely contribute to disease pathogenesis and, therefore, may correlate with symptoms better than PEC. We sought to determine whether symptoms in patients with EoE more closely relate to eosinophil granule protein deposition than to eosinophil enumeration, especially in patients with fewer than 15 eosinophils per high power field (HPF). Esophageal biopsy specimens from 34 patients diagnosed with EoE were obtained for histopathological examination and for evaluation of eMBP1 staining by indirect immunofluorescence. PEC by histopathology were compared to extracellular eMBP1 grades by immunostaining. PEC and eMBP1 grades also were analyzed for their relationship to symptoms and clinical course. Biopsy specimens from 19 of the 34 patients had fewer than 15 PEC on histopathological examination, and the other 15 patients had 15 or greater PEC. Positive eMBP1 immunostaining was found in all symptomatic patients. EoE symptoms were related to eMBP1 immunostaining grades (p = 0.0001), but not PEC (P = 0.14). Eosinophil granule protein deposition, specifically eMBP1, is increased in esophageal biopsy specimens from symptomatic patients with EoE and may be a marker of disease activity, including patients with EoE who have ‘resolved’ disease.

Mo1828 Major Basic Protein, Eotaxin-3, and Mast Cell Tryptase Staining for Diagnosis of Eosinophilic Esophagitis: A Prospective Study

2014 ◽  
Vol 146 (5) ◽  
pp. S-664
Author(s):  
Evan S. Dellon ◽  
Olga Speck ◽  
Kimberly Woodward ◽  
Shannon Covey ◽  
Spencer Rusin ◽  
...  
Keyword(s):  
Mast Cell ◽  
Prospective Study ◽  
Eosinophilic Esophagitis ◽  
Basic Protein ◽  
Major Basic Protein ◽  
Mast Cell Tryptase ◽  
A Prospective Study
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