Determination of protease cleavage site motifs using mixture-based oriented peptide libraries

10.1038/90273 ◽  
2001 ◽  
Vol 19 (7) ◽  
pp. 661-667 ◽  
Author(s):  
Benjamin E. Turk ◽  
Lisa L. Huang ◽  
Elizabeth T. Piro ◽  
Lewis C. Cantley
Keyword(s):  
Cleavage Site ◽  
Peptide Libraries ◽  
Protease Cleavage Site ◽  
Protease Cleavage

scholarly journals Proteolytic Cleavage of Bovine Adenovirus 3-Encoded pVIII

2017 ◽  
Vol 91 (10) ◽  
Author(s):  
Amit Gaba ◽  
Lisanework Ayalew ◽  
Niraj Makadiya ◽  
Suresh Tikoo
Keyword(s):  
Cleavage Site ◽  
Virus Assembly ◽  
Proteolytic Cleavage ◽  
Efficient Production ◽  
Cleavage Sites ◽  
Bovine Adenovirus ◽  
Protease Cleavage Site ◽  
Protease Cleavage ◽  
Protease Cleavage Sites ◽  
Single Protease

ABSTRACT Proteolytic maturation involving cleavage of one nonstructural and six structural precursor proteins including pVIII by adenovirus protease is an important aspect of the adenovirus life cycle. The pVIII encoded by bovine adenovirus 3 (BAdV-3) is a protein of 216 amino acids and contains two potential protease cleavage sites. Here, we report that BAdV-3 pVIII is cleaved by adenovirus protease at both potential consensus protease cleavage sites. Usage of at least one cleavage site appears essential for the production of progeny BAdV-3 virions as glycine-to-alanine mutation of both protease cleavage sites appears lethal for the production of progeny virions. However, mutation of a single protease cleavage site of BAdV-3 pVIII significantly affects the efficient production of infectious progeny virions. Further analysis revealed no significant defect in endosome escape, genome replication, capsid formation, and virus assembly. Interestingly, cleavage of pVIII at both potential cleavage sites appears essential for the production of stable BAdV-3 virions as BAdV-3 expressing pVIII containing a glycine-to-alanine mutation of either of the potential cleavage sites is thermolabile, and this mutation leads to the production of noninfectious virions. IMPORTANCE Here, we demonstrated that the BAdV-3 adenovirus protease cleaves BAdV-3 pVIII at both potential protease cleavage sites. Although cleavage of pVIII at one of the two adenoviral protease cleavage sites is required for the production of progeny virions, the mutation of a single cleavage site of pVIII affects the efficient production of infectious progeny virions. Further analysis indicated that the mutation of a single protease cleavage site (glycine to alanine) of pVIII produces thermolabile virions, which leads to the production of noninfectious virions with disrupted capsids. We thus provide evidence about the requirement of proteolytic cleavage of pVIII for production of infectious progeny virions. We feel that our study has significantly advanced the understanding of the requirement of adenovirus protease cleavage of pVIII.

scholarly journals Thegagprecursor contains a specific HIV-1 protease cleavage site between the NC (P7) and P1 proteins

FEBS Letters ◽  
1993 ◽  
Vol 333 (1-2) ◽  
pp. 21-24 ◽  
Author(s):  
Ewald M. Wondrak ◽  
John M. Louis ◽  
Hugues de Rocquigny ◽  
Jean-Claude Chermann ◽  
Bernard P. Roques
Keyword(s):  
Cleavage Site ◽  
Protease Cleavage Site ◽  
Protease Cleavage ◽  
Hiv 1

scholarly journals Development of a novel anti-HIV-1 agent from within: Effect of chimeric Vpr-containing protease cleavage site residues on virus replication

1997 ◽  
Vol 94 (7) ◽  
pp. 3346-3351 ◽  
Author(s):  
D. Serio ◽  
T. A. Rizvi ◽  
M. Cartas ◽  
V. S. Kalyanaraman ◽  
I. T. Weber ◽  
...  
Keyword(s):  
Virus Replication ◽  
Cleavage Site ◽  
Protease Cleavage Site ◽  
Protease Cleavage ◽  
Anti Hiv ◽  
Hiv 1

scholarly journals MBP Fusion Protein with a Viral Protease Cleavage Site: One-Step Cleavage/Purification of Insoluble Proteins

BioTechniques ◽  
2001 ◽  
Vol 30 (6) ◽  
pp. 1194-1198 ◽  
Author(s):  
Andrei Alexandrov ◽  
Kaushik Dutta ◽  
Steven M. Pascal
Keyword(s):  
Fusion Protein ◽  
Cleavage Site ◽  
Viral Protease ◽  
Protease Cleavage Site ◽  
Protease Cleavage ◽  
One Step

scholarly journals Glycine insertion at protease cleavage site of SNAP25 resists cleavage but enhances affinity for botulinum neurotoxin serotype A

2012 ◽  
Vol 21 (3) ◽  
pp. 318-326 ◽  
Author(s):  
Mengfei Ho ◽  
Cheong-Hian Goh ◽  
Michael C. Brothers ◽  
Shihua Wang ◽  
Ryan L. Young ◽  
...  
Keyword(s):  
Cleavage Site ◽  
Botulinum Neurotoxin ◽  
Serotype A ◽  
Protease Cleavage Site ◽  
Protease Cleavage ◽  
Botulinum Neurotoxin Serotype A

scholarly journals Mutation in the protease cleavage site ofGDF9increases ovulation rate and litter size in heterozygous ewes and causes infertility in homozygous ewes

2014 ◽  
Vol 45 (5) ◽  
pp. 732-739 ◽  
Author(s):  
C. J. H. Souza ◽  
A. S. McNeilly ◽  
M. V. Benavides ◽  
E. O. Melo ◽  
J. C. F. Moraes
Keyword(s):  
Litter Size ◽  
Cleavage Site ◽  
Ovulation Rate ◽  
Protease Cleavage Site ◽  
Protease Cleavage
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