11-color, 13-parameter flow cytometry: Identification of human naive T cells by phenotype, function, and T-cell receptor diversity

10.1038/84701 ◽  
2001 ◽  
Vol 7 (2) ◽  
pp. 245-248 ◽  
Author(s):  
Stephen C. De Rosa ◽  
Leonard A. Herzenberg ◽  
Leonore A. Herzenberg ◽  
Mario Roederer
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Naive T Cells ◽  
Naïve T Cells

Naive T cells can mediate delayed-type hypersensitivity response in T cell receptor transgenic mice

1994 ◽  
Vol 24 (7) ◽  
pp. 1512-1516 ◽  
Author(s):  
Takehito Sato ◽  
Takeshi Sasahara ◽  
Yukitsugu Nakamura ◽  
Takako Osaki ◽  
Takanori Hasegawa ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Transgenic Mice ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Delayed Type Hypersensitivity ◽  
Hypersensitivity Response ◽  
Naive T Cells ◽  
Naïve T Cells ◽  
Delayed Type Hypersensitivity Response

T cell-induced inflammation of the small and large intestine in immunodeficient mice

2006 ◽  
Vol 290 (1) ◽  
pp. G109-G119 ◽  
Author(s):  
Dmitry V. Ostanin ◽  
Kevin P. Pavlick ◽  
Sulaiman Bharwani ◽  
Dwain D′Souza ◽  
Kathryn L. Furr ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Small Bowel ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Cell Transfer ◽  
Chronic Colitis ◽  
Naive T Cells ◽  
Naïve T Cells ◽  
T Cell Transfer

It is well known that transfer of CD4+CD45RBhigh (naïve) T cells into syngeneic lymphocyte-deficient mice induces chronic colitis. However, no studies have reported the presence of small bowel inflammation in this T cell-dependent model. Therefore, the objective of this study was to evaluate and compare small and large bowel inflammation induced by transfer of naïve T cells into two different immunodeficient recipient mice. T and B cell-deficient recombinase activating gene 1-deficient [RAG knockout (KO)] and T cell-deficient T cell receptor-β × T cell receptor-δ double-deficient (TCR KO) mice were reconstituted with wild-type naïve T cells and observed for signs of disease. We found that reconstituted RAG KO mice developed moderate to severe colitis and inflammation of the entire small intestine at 6–8 wk after T cell transfer. Adoptive transfer of naïve T cells into TCR KO mice induced a milder form of chronic colitis and small bowel inflammation that was confined primarily to the duodenum at 10–12 wk after T cell transfer. T helper cell 1 and macrophage-derived proinflammatory cytokine mRNA levels correlated well with the localization and severity of the chronic large and small bowel inflammation. In addition, we observed comparable homing and expansion of donor lymphocytes in the gut and secondary lymphoid tissues of both recipients. Taken together, our data demonstrate that transfer of naïve T cells into immunodeficient recipient mice induces both chronic small and large bowel inflammation and that the presence of B cells in the TCR KO recipients may play a role in regulating chronic intestinal inflammation.

scholarly journals Trends in TRECs values according to age and gender in Chinese children, and their clinical application

2021 ◽  
Author(s):  
Qin Zhao ◽  
Xiao-Dong Zhao
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Chinese Children ◽  
Hematopoietic Stem ◽  
Naive T Cells ◽  
Cell Numbers ◽  
Naïve T Cells ◽  
And Gender

T cell receptor excision circles (TRECs) are small circularized DNA elements produced during rearrangement of T cell receptor (TCR) genes. Because TRECS are fairly stable, do not replicate during mitosis, and are not diluted during division of naïve T cells1, they are suitable for assessing the number of newly formed T cells 2. In this study, we detected TRECs in 475 healthy Chinese children aged 0–18 years in different clinical settings. We found a strong correlation between TRECs levels and peripheral CD4 naïve T cell numbers, but not between TRECs levels and effector or memory CD4 and CD8 T cell numbers. TRECs levels fell significantly compared with normal controls in patients with severe combined immunodeficiencies (SCID) (n=7), wiskott-aldrich syndrome (WAS) (n=22), or activated PI3Kδ syndrome (APDS) (n=5). TRECs levels in those with signal transducer and activator of transcription 1 (STAT1) deficiency (n=8) decreased or did not change significantly, a finding consistent with that for CD4 naïve T cells. We also measured TRECs levels in seven PIDs after hematopoietic stem cell transplantation (HSCT) (WAS=5; chronic granulomatous disease (CGD)=2), and found the complications after HSCT may reduce TRECs levels by interfering with production of naïve T cells. In conclusion, we established reference values for TRECs, which can be used to screen for primary immunodeficiency diseases (PIDs) during early life and track immune reconstitution after HSCT.

Peptide-induced T cell receptor down-regulation on naive T cells predicts agonist/partial agonist properties and strictly correlates with T cell activation

1997 ◽  
Vol 27 (9) ◽  
pp. 2195-2203 ◽  
Author(s):  
Martin F. Bachmann ◽  
Annette Oxenius ◽  
Daniel E. Speiser ◽  
Sanjeev Mariathasan ◽  
Hans Hengartner ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
T Cell Activation ◽  
Cell Activation ◽  
Partial Agonist ◽  
Cell Receptor ◽  
Down Regulation ◽  
Naive T Cells ◽  
Naïve T Cells

scholarly journals CD95 co-stimulation blocks activation of naive T cells by inhibiting T cell receptor signaling

2009 ◽  
Vol 185 (6) ◽  
pp. i13-i13
Author(s):  
Gudrun Strauss ◽  
Jonathan A. Lindquist ◽  
Nathalie Arhel ◽  
Edward Felder ◽  
Sabine Karl ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Receptor Signaling ◽  
Naive T Cells ◽  
Naïve T Cells ◽  
T Cell Receptor Signaling ◽  
Cell Receptor Signaling

scholarly journals The Combined Effects of Tryptophan Starvation and Tryptophan Catabolites Down-Regulate T Cell Receptor ζ-Chain and Induce a Regulatory Phenotype in Naive T Cells

2006 ◽  
Vol 176 (11) ◽  
pp. 6752-6761 ◽  
Author(s):  
Francesca Fallarino ◽  
Ursula Grohmann ◽  
Sylvaine You ◽  
Barbara C. McGrath ◽  
Douglas R. Cavener ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Combined Effects ◽  
Naive T Cells ◽  
Naïve T Cells ◽  
Tryptophan Catabolites ◽  
Regulatory Phenotype

scholarly journals Recombinant human IL‐7 (rhIL‐7) preferentially expands CD4+ and CD8+ naïve T cells and enhances T cell receptor (TCR) repertoire diversity

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Claude Sportes ◽  
Frances T Hakim ◽  
Sarfraz A Memon ◽  
Hua Zhang ◽  
Margaret R Brown ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Naive T Cells ◽  
Naïve T Cells ◽  
Tcr Repertoire ◽  
Repertoire Diversity
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