A single adenovirus vector mediates doxycycline-controlled expression of tyrosine hydroxylase in brain grafts of human neural progenitors

10.1038/7901 ◽  
1999 ◽  
Vol 17 (4) ◽  
pp. 349-354 ◽  
Author(s):  
Olga Corti ◽  
Olivier Sabaté ◽  
Philippe Horellou ◽  
Philippe Colin ◽  
Sylvie Dumas ◽  
...  
Keyword(s):  
Tyrosine Hydroxylase ◽  
Adenovirus Vector ◽  
Neural Progenitors ◽  
Brain Grafts

scholarly journals Nicotinamide restricts neural precursor proliferation to enhance catecholaminergic neuronal subtype differentiation from mouse embryonic stem cells

2020 ◽  
Author(s):  
Síle M. Griffin ◽  
Mark R. Pickard ◽  
Clive P. Hawkins ◽  
Adrian C. Williams ◽  
Rosemary A. Fricker
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Tyrosine Hydroxylase ◽  
Cell Cultures ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Neural Progenitors ◽  
Neuronal Populations ◽  
Regenerative Therapies

AbstractEmerging evidence indicates that a strong relationship exists between brain regenerative therapies and nutrition. Early life nutrition plays an important role during embryonic brain development, and there are clear consequences to an imbalance in nutritional factors on both the production and survival of mature neuronal populations and the infant’s risk of diseases in later life. Our research and that of others suggest that vitamins play a fundamental role in the formation of neurons and their survival. There is a growing body of evidence that nicotinamide, the water-soluble amide form of vitamin B3, is implicated in the conversion of pluripotent stem cells to clinically relevant cells for regenerative therapies. This study investigated the ability of nicotinamide to promote the development of mature catecholaminergic neuronal populations (associated with Parkinson’s disease) from mouse embryonic stem cells, as well as investigating the underlying mechanisms of nicotinamide’s action.Nicotinamide selectively enhanced the production of tyrosine hydroxylase-expressing neurons and serotonergic neurons from mouse embryonic stem cell cultures (Sox1GFP knock-in 46C cell line). A 5-Ethynyl-2’-deoxyuridine (EdU) assay ascertained that nicotinamide, when added in the initial phase, reduced cell proliferation. Nicotinamide drove tyrosine hydroxylase-expressing neuron differentiation as effectively as an established cocktail of signalling factors, reducing the proliferation of neural progenitors and accelerating neuronal maturation, neurite outgrowth and neurotransmitter expression.These novel findings show that nicotinamide enhanced and enriched catecholaminergic differentiation and inhibited cell proliferation by directing cell cycle arrest in mouse embryonic stem cell cultures, thus driving a critical neural proliferation-to-differentiation switch from neural progenitors to neurons. Further research into the role of vitamin metabolites in embryogenesis will significantly advance cell-based regenerative medicine, and help realize their role as crucial developmental signalling molecules in brain development.

scholarly journals Transfection of human lactotroph adenoma cells with an adenovirus vector expressing tyrosine hydroxylase decreases prolactin release.

1996 ◽  
Vol 81 (6) ◽  
pp. 2401-2404
Author(s):  
A Freese ◽  
M J During ◽  
B L Davidson ◽  
T A Gennarelli ◽  
M G Kaplitt ◽  
...  
Keyword(s):  
Tyrosine Hydroxylase ◽  
Adenovirus Vector ◽  
Prolactin Release

Colocalization of tyrosine hydroxylase and GAD65 mRNA in mesostriatal neurons

2001 ◽  
Vol 13 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Tomas Gonzalez-Hernandez ◽  
Pedro Barroso-Chinea ◽  
Abraham Acevedo ◽  
Eduardo Salido ◽  
Manuel Rodriguez
Keyword(s):  
Tyrosine Hydroxylase
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