scholarly journals Hospital closure puts tissue bank in jeopardy

Nature ◽  
2005 ◽  
Vol 437 (7055) ◽  
pp. 7-7 ◽  
Author(s):  
Geoff Brumfiel
Keyword(s):  
Tissue Bank ◽  
Hospital Closure

Correlative microscopy in distrubuted (client/server) computing environments: tools for catalyzing the rapid dissemination of information about the cell biology of the human prostate

1995 ◽  
Vol 53 ◽  
pp. 682-683
Author(s):  
A. Hakam ◽  
J.T. Gau ◽  
M.L. Grove ◽  
B.A. Evans ◽  
M. Shuman ◽  
...  
Keyword(s):  
United States ◽  
Cell Biology ◽  
Tissue Bank ◽  
The United States ◽  
Prostate Adenocarcinoma ◽  
Correlative Microscopy ◽  
Client Server ◽  
Critical Elements ◽  
Transplant Organ

Prostate adenocarcinoma is the most common malignant tumor of men in the United States and is the third leading cause of death in men. Despite attempts at early detection, there will be 244,000 new cases and 44,000 deaths from the disease in the United States in 1995. Therapeutic progress against this disease is hindered by an incomplete understanding of prostate epithelial cell biology, the availability of human tissues for in vitro experimentation, slow dissemination of information between prostate cancer research teams and the increasing pressure to “ stretch” research dollars at the same time staff reductions are occurring.To meet these challenges, we have used the correlative microscopy (CM) and client/server (C/S) computing to increase productivity while decreasing costs. Critical elements of our program are as follows:1) Establishing the Western Pennsylvania Genitourinary (GU) Tissue Bank which includes >100 prostates from patients with prostate adenocarcinoma as well as >20 normal prostates from transplant organ donors.

Hospital Closure and the Creation of Community Settings

1996 ◽  
Vol 41 (2) ◽  
pp. 169-170
Author(s):  
Michael B. Blank ◽  
Marlene M. Eisenberg
Keyword(s):  
Community Settings ◽  
The Creation ◽  
Hospital Closure

UCLA/USC Tumor Tissue Bank.

1996 ◽  
Author(s):  
Dennis J. Salmon
Keyword(s):  
Tumor Tissue ◽  
Tissue Bank

scholarly journals HPV Strain Predicts Severity of Juvenile-Onset Recurrent Respiratory Papillomatosis with Implications for Disease Screening

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2556
Author(s):  
Mary C. Bedard ◽  
Alessandro de Alarcon ◽  
Yann-Fuu Kou ◽  
David Lee ◽  
Alexandra Sestito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Severity ◽  
Disease Onset ◽  
Tissue Bank ◽  
Benign Neoplasm ◽  
Recurrent Respiratory Papillomatosis ◽  
Clinical Severity ◽  
Viral Gene ◽  
Juvenile Onset ◽  
Severity Scores

Juvenile-onset recurrent respiratory papillomatosis (JoRRP) is the most common benign neoplasm of the larynx in children, presenting with significant variation in clinical course and potential for progression to malignancy. Since JoRRP is driven by human papillomavirus (HPV), we evaluated viral factors in a prospective cohort to identify predictive factors of disease severity. Twenty children with JoRRP undergoing routine debridement of papillomas were recruited and followed for ≥1 year. Demographical features, clinical severity scores, and surgeries over time were tabulated. Biopsies were used to establish a tissue bank and primary cell cultures for HPV6 vs. HPV11 genotyping and evaluation of viral gene expression. We found that patients with HPV11+ disease had an earlier age at disease onset, higher frequency of surgeries, increased number of lifetime surgeries, and were more likely to progress to malignancy. However, the amplitude of viral E6/E7 gene expression did not account for increased disease severity in HPV11+ patients. Determination of HPV strain is not routinely performed in the standard of care for JoRRP patients; we demonstrate the utility and feasibility of HPV genotyping using RNA-ISH for screening of HPV11+ disease as a biomarker for disease severity and progression in JoRRP patients.

Abstract 427: Alterations in the Mitochondrial Supercomplex in Pediatric Dilated Cardiomyopathy

2017 ◽  
Vol 121 (suppl_1) ◽  
Author(s):  
Kathryn C Chatfield ◽  
Genevieve C Sparagna ◽  
Sarah Chau ◽  
Michael J Bennett ◽  
Adam J Chicco ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Dilated Cardiomyopathy ◽  
Phospholipid Composition ◽  
Tissue Bank ◽  
Fatty Acyl ◽  
Energy Utilization ◽  
Physical Interaction ◽  
Inner Mitochondrial Membrane ◽  
Cross Sectional ◽  
Mitochondrial Fatty Acid

Organization of the mitochondrial electron transport chain (ETC) into a protein “supercomplex” has been shown to be critical for optimal mitochondrial respiration, and is dependent on the phospholipid composition of the inner mitochondrial membrane. A close physical interaction between the ETC supercomplex and the fatty acid beta-oxidation system (FAO, which provides necessary reducing equivalents for ETC) has also been proposed. We have previously demonstrated that content of the primary mitochondrial phospholipid, cardiolipin, is altered in pediatric dilated cardiomyopathy (DCM), with evidence for its dysregulated biosynthesis. We hypothesized that altered cardiolipin content in pediatric DCM is correlated with altered supercomplex-associated ETC activity and mitochondrial fatty acid β-oxidation. A cross-sectional investigation was performed using myocardium from 16 children with DCM and 15 non-failing (NF) controls from the University of Colorado Heart Tissue Bank. Using blue native (BN) -PAGE with in-gel activity staining we demonstrated lower activity of supercomplex-associated complexes I (DCM 80% of NF, P<0.05) and IV (DCM 72% of NF, P<0.05) in pediatric DCM compared with NF controls. Using BN-PAGE and Western blot, as well as proteomic analysis of isolated supercomplex bands, we demonstrated interaction of the ETC supercomplex with FAO enzymes. Quantification of fatty acyl-CoAs was also performed in tissue from pediatric patients with DCM which demonstrated altered content of a subset of acyl-CoAs when compared to NF controls. We detected higher content of some C8, C10 and C12 CoAs in DCM compared with NF (P<0.05), with depletion of C18:1, C18:2, and C16 species (P<0.05). There was no difference between groups in free CoA or Acetyl-CoA. Taken together, these data suggest a potentially important interaction between the ETC supercomplex and long-chain β-oxidation enzymes, which may be altered on heart failure. We provide preliminary evidence for disrupted energy utilization in the failing pediatric heart.

scholarly journals The Genome Austria Tissue Bank (GATiB)

Pathobiology ◽  
2007 ◽  
Vol 74 (4) ◽  
pp. 251-258 ◽  
Author(s):  
M. Asslaber ◽  
P.M. Abuja ◽  
K. Stark ◽  
J. Eder ◽  
H. Gottweis ◽  
...  
Keyword(s):  
Tissue Bank

Skin donors and human skin allografts: evaluation of an 11-year practice and discard in a referral tissue bank

2015 ◽  
Vol 17 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Sonia Gaucher ◽  
Zena Khaznadar ◽  
Jean-Claude Gourevitch ◽  
Mohamed Jarraya
Keyword(s):  
Human Skin ◽  
Tissue Bank ◽  
Skin Allografts ◽  
Skin Donors

scholarly journals Molecular subtyping improves prognostication of Stage 2 colorectal cancer

2019 ◽  
Author(s):  
Rachel Violet Purcell ◽  
Sebastian Schmeier ◽  
Yee Chen Lau ◽  
John F Pearson ◽  
Francis A Frizelle
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Tissue Bank ◽  
Prognostic Indicator ◽  
Good Prognosis ◽  
Tnm Staging ◽  
Tumour Suppressor Genes ◽  
Molecular Subtyping ◽  
Prognostic Effect ◽  
Treatment Naïve

Abstract Background: Post-surgical staging is the mainstay of prognostic stratification for colorectal cancer (CRC). Here, we compare TNM staging to consensus molecular subtyping (CMS) and assess the value of subtyping in addition to stratification by TNM. Methods: Three hundred and eight treatment-naïve colorectal tumours were accessed from our institutional tissue bank. CMS typing was carried out using tumour gene-expression data. Post-surgical TNM-staging and CMS were analysed with respect to clinicopathologic variables and patient outcome. Results : CMS alone was not associated with survival, while TNM stage significantly explained mortality. Addition of CMS to TNM-stratified tumours showed a prognostic effect in stage 2 tumours; CMS3 tumours had a significantly lower overall survival (P = 0.006). Stage 2 patients with a good prognosis showed immune activation and up-regulation of tumour suppressor genes. Conclusions : Although stratification using CMS does not outperform TNM staging as a prognostic indicator, gene-expression based subtyping shows promise for improved prognostication in stage 2 CRC.

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