Differentiation of adult hippocampus-derived progenitors into olfactory neurons in vivo

Nature ◽  
1996 ◽  
Vol 383 (6601) ◽  
pp. 624-627 ◽  
Author(s):  
Jaana O. Suhonen ◽  
Daniel A. Peterson ◽  
Jasodhara Ray ◽  
Fred H. Gage
Keyword(s):  
Olfactory Neurons ◽  
Adult Hippocampus

scholarly journals Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

10.3791/3380 ◽  
2011 ◽  
Author(s):  
Gabriel Lepousez ◽  
Mariana Alonso ◽  
Sebastian Wagner ◽  
Benjamin W. Gallarda ◽  
Pierre-Marie Lledo
Keyword(s):  
Olfactory Neurons ◽  
Optogenetic Stimulation ◽  
Viral Transduction

scholarly journals Effects of Tokishakuyakusan on Regeneration of Murine Olfactory Neurons In Vivo and In Vitro

2019 ◽  
Vol 44 (5) ◽  
pp. 327-338 ◽  
Author(s):  
Takuya Noda ◽  
Hideaki Shiga ◽  
Kentaro Yamada ◽  
Masayuki Harita ◽  
Yukari Nakamura ◽  
...  
Keyword(s):  
Olfactory Neurons

scholarly journals N-cadherin regulates molecular organization of excitatory and inhibitory synaptic circuits in adult hippocampus in vivo

Hippocampus ◽  
2014 ◽  
Vol 24 (8) ◽  
pp. 943-962 ◽  
Author(s):  
Jessica S. Nikitczuk ◽  
Shekhar B. Patil ◽  
Bridget A. Matikainen-Ankney ◽  
Joseph Scarpa ◽  
Matthew L. Shapiro ◽  
...  
Keyword(s):  
Molecular Organization ◽  
Synaptic Circuits ◽  
Adult Hippocampus

scholarly journals Bidirectional, Activity-Dependent Regulation of Glutamate Receptors in the Adult Hippocampus In Vivo

Neuron ◽  
2000 ◽  
Vol 28 (2) ◽  
pp. 527-536 ◽  
Author(s):  
Arnold J. Heynen ◽  
Elizabeth M. Quinlan ◽  
David C. Bae ◽  
Mark F. Bear
Keyword(s):  
Glutamate Receptors ◽  
Adult Hippocampus ◽  
Activity Dependent

scholarly journals Environmental CO2 inhibits Caenorhabditis elegans egg-laying by modulating olfactory neurons and evokes widespread changes in neural activity

2015 ◽  
Vol 112 (27) ◽  
pp. E3525-E3534 ◽  
Author(s):  
Lorenz A. Fenk ◽  
Mario de Bono
Keyword(s):  
Caenorhabditis Elegans ◽  
Sensory Processing ◽  
Egg Laying ◽  
Olfactory Neurons ◽  
Sensory Pathways ◽  
Sensory Cue ◽  
Gustatory Neurons ◽  
Sensory Endings ◽  
Dependent Mechanism

Carbon dioxide (CO2) gradients are ubiquitous and provide animals with information about their environment, such as the potential presence of prey or predators. The nematode Caenorhabditis elegans avoids elevated CO2, and previous work identified three neuron pairs called “BAG,” “AFD,” and “ASE” that respond to CO2 stimuli. Using in vivo Ca2+ imaging and behavioral analysis, we show that C. elegans can detect CO2 independently of these sensory pathways. Many of the C. elegans sensory neurons we examined, including the AWC olfactory neurons, the ASJ and ASK gustatory neurons, and the ASH and ADL nociceptors, respond to a rise in CO2 with a rise in Ca2+. In contrast, glial sheath cells harboring the sensory endings of C. elegans’ major chemosensory neurons exhibit strong and sustained decreases in Ca2+ in response to high CO2. Some of these CO2 responses appear to be cell intrinsic. Worms therefore may couple detection of CO2 to that of other cues at the earliest stages of sensory processing. We show that C. elegans persistently suppresses oviposition at high CO2. Hermaphrodite-specific neurons (HSNs), the executive neurons driving egg-laying, are tonically inhibited when CO2 is elevated. CO2 modulates the egg-laying system partly through the AWC olfactory neurons: High CO2 tonically activates AWC by a cGMP-dependent mechanism, and AWC output inhibits the HSNs. Our work shows that CO2 is a more complex sensory cue for C. elegans than previously thought, both in terms of behavior and neural circuitry.

scholarly journals The Microtubule Severing Protein Katanin Regulates Proliferation of Neuronal Progenitors in Embryonic and Adult Neurogenesis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Franco L. Lombino ◽  
Mary Muhia ◽  
Jeffrey Lopez-Rojas ◽  
Monika S. Brill ◽  
Edda Thies ◽  
...  
Keyword(s):  
Adult Neurogenesis ◽  
Critical Role ◽  
Mammalian Brain ◽  
Cellular Functions ◽  
Microtubule Severing ◽  
Neuronal Progenitor ◽  
Neuronal Progenitors ◽  
Adult Hippocampus

Abstract Microtubule severing regulates cytoskeletal rearrangement underlying various cellular functions. Katanin, a heterodimer, consisting of catalytic (p60) and regulatory (p80) subunits severs dynamic microtubules to modulate several stages of cell division. The role of p60 katanin in the mammalian brain with respect to embryonic and adult neurogenesis is poorly understood. Here, we generated a Katna1 knockout mouse and found that consistent with a critical role of katanin in mitosis, constitutive homozygous Katna1 depletion is lethal. Katanin p60 haploinsufficiency induced an accumulation of neuronal progenitors in the subventricular zone during corticogenesis, and impaired their proliferation in the adult hippocampus dentate gyrus (DG) subgranular zone. This did not compromise DG plasticity or spatial and contextual learning and memory tasks employed in our study, consistent with the interpretation that adult neurogenesis may be associated with selective forms of hippocampal-dependent cognitive processes. Our data identify a critical role for the microtubule-severing protein katanin p60 in regulating neuronal progenitor proliferation in vivo during embryonic development and adult neurogenesis.

scholarly journals In Vivo Fate Analysis Reveals the Multipotent and Self-Renewal Capacities of Sox2+ Neural Stem Cells in the Adult Hippocampus

2007 ◽  
Vol 1 (5) ◽  
pp. 515-528 ◽  
Author(s):  
Hoonkyo Suh ◽  
Antonella Consiglio ◽  
Jasodhara Ray ◽  
Toru Sawai ◽  
Kevin A. D'Amour ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Self Renewal ◽  
Adult Hippocampus

scholarly journals Transcriptional regulation of neural stem cell expansion in adult hippocampus

2021 ◽  
Author(s):  
Nannan Guo ◽  
Kelsey McDermott ◽  
Yu-Tzu Shih ◽  
Haley Zanga ◽  
Debolina Ghosh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Transcription Factors ◽  
Neural Stem Cell ◽  
Cell Expansion ◽  
Self Renewal ◽  
Stem Cell Expansion ◽  
Radial Glial ◽  
Adult Hippocampus ◽  
Favorable Conditions

Experience governs neurogenesis from radial-glial neural stem cells (RGLs) in the adult hippocampus to support memory. Transcription factors in RGLs integrate physiological signals to dictate self-renewal division mode. Whereas asymmetric RGL divisions drive neurogenesis during favorable conditions, symmetric divisions prevent premature neurogenesis while amplifying RGLs to anticipate future neurogenic demands. The identities of transcription factors regulating RGL symmetric self-renewal, unlike those that regulate RGL asymmetric self-renewal, are not known. Here, we show that the transcription factor Kruppel-like factor 9 (Klf9) is elevated in quiescent RGLs and inducible, deletion of Klf9 promotes RGL activation state. Clonal analysis and longitudinal intravital 2-photon imaging directly demonstrate that Klf9 functions as a brake on RGL symmetric self-renewal. In vivo translational profiling of RGLs lacking Klf9 generated a blueprint of RGL symmetric self-renewal for stem cell community. Together, these observations identify Klf9 as a transcriptional regulator of neural stem cell expansion in the adult hippocampus.

scholarly journals Non-canonical odor coding ensures unbreakable mosquito attraction to humans

2020 ◽  
Author(s):  
Meg A. Younger ◽  
Margaret Herre ◽  
Alison R. Ehrlich ◽  
Zhongyan Gong ◽  
Zachary N. Gilbert ◽  
...  
Keyword(s):  
Olfactory System ◽  
Receptor Gene ◽  
Gene Families ◽  
Receptor Expression ◽  
Olfactory Neurons ◽  
Odor Cues ◽  
Chemosensory Receptor ◽  
Single Receptor ◽  
The Brain

SUMMARYFemale Aedes aegypti mosquitoes show strong innate attraction to humans. This chemosensory behavior is critical to species survival because females require a blood-meal to reproduce. Humans, the preferred host of Ae. aegypti, produce a complex blend of odor cues along with carbon dioxide (CO2) that attracts females ready to bite. Mosquitoes detect these cues with heteromeric ligand-gated ion channels encoded by three different chemosensory receptor gene families. A common theme in other species is that olfactory neurons express a single receptor that defines their chemical specificity and that they extend axons that converge upon dedicated glomeruli in the first sensory processing center in the brain. Such an organization permits the brain to segregate olfactory information and monitor activity of individual glomeruli to interpret what smell has been encountered. We have discovered that Ae. aegypti uses an entirely different organizational principle for its olfactory system. Using genetic strains that label subpopulations of olfactory neurons, we found that many neurons co-express multiple members of at least two of the chemosensory receptor families. This unexpected co-expression is functional, as assessed by in vivo calcium imaging showing that a given glomerulus is activated by multiple ligands detected by different receptor families. This has direct functional consequences for mosquito behavior. Mutant mosquitoes that cannot sense CO2 can be behaviorally activated by a volatile amine that stimulates the CO2 glomerulus. This non-canonical olfactory system organization featuring overlapping receptor expression may explain the female mosquito’s robust and “unbreakable’ attraction to humans.

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