Identification of a mouse male-specific transplantation antigen, H-Y

Nature ◽  
1995 ◽  
Vol 376 (6542) ◽  
pp. 695-698 ◽  
Author(s):  
D. M. Scott ◽  
I. E. Ehrmann ◽  
P. S. Ellis ◽  
C. E. Bishop ◽  
A. I. Agulnik ◽  
...  
Keyword(s):  
Transplantation Antigen ◽  
Male Specific

DFFRY codes for a new human male-specific minor transplantation antigen involved in bone marrow graft rejection

Blood ◽  
2000 ◽  
Vol 95 (3) ◽  
pp. 1100-1105 ◽  
Author(s):  
M. H. J. Vogt ◽  
R. A. de Paus ◽  
P. J. Voogt ◽  
R. Willemze ◽  
J. H. F. Falkenburg
Keyword(s):  
Bone Marrow ◽  
Y Chromosome ◽  
Graft Rejection ◽  
Transplantation Antigen ◽  
Target Cells ◽  
Specific Gene ◽  
Marrow Graft ◽  
Minor Histocompatibility Antigens ◽  
Bone Marrow Graft ◽  
Male Specific

Graft rejection after histocompatibility locus antigen (HLA)-identical stem cell transplantation results from the recognition of minor histocompatibility antigens on donor stem cells by immunocompetent T lymphocytes of recipient origin. T-lymphocyte clones that specifically recognize H-Y epitopes on male target cells have been generated during graft rejection after sex-mismatched transplantation. Previously, 2 human H-Y epitopes derived from the same SMCY gene have been identified that were involved in bone marrow graft rejection. We report the identification of a new male-specific transplantation antigen encoded by the Y-chromosome-specific gene DFFRY. The DFFRY-derived peptide was recognized by an HLA-A1 restricted CTL clone, generated during graft rejection from a female patient with acute myeloid leukemia who rejected HLA-phenotypically identical bone marrow from her father. The identification of this gene demonstrates that at least 2 genes present on the human Y-chromosome code for male-specific transplantation antigens.

On the evolution of sex determination

1987 ◽  
Vol 232 (1267) ◽  
pp. 159-180 ◽  
Keyword(s):  
Sex Determination ◽  
Sex Chromosome ◽  
Transplantation Antigen ◽  
Specific Cell ◽  
Serological Tests ◽  
Specific Expression ◽  
Heterogametic Sex ◽  
Taxonomic Groups ◽  
Male Specific ◽  
Specific Cell Surface

Female mice reject skin grafts from intrastrain males because of the H-Y transplantation antigen. Those females produce antibodies that recognize a male-specific cell-surface antigen in serological tests. The serological antigen has also been called ‘H-Y’, but there is evidence that the two antigens are distinct. We therefore refer to the transplantation antigen as H-Yt, or transplantation H-Y, and to the serological antigen as serological H-Y, or simply H-Y, without prejudice whether these are the same or related or separate antigens. In this study, sex-specific expression of serological H-Y antigen was found in 25 new vertebrate species representing each of seven major vertebrate classes.There was a strong correlation between expression of H-Y and occurrence of the heterogametic-type gonad, although unusual patterns of H-Y expression were noted in cases of temperature-influenced sex determination and in systems representing possible transition from one mode of heterogamety to the other. Male and female heterogamety are found side-by-side in certain freshwater toothed carps; and distinct sex chromosomes have been recognized in certain amphibians, even though they are not apparent in certain reptiles and primitíve birds. In seven ophidian species, in which the female is the heterogametic sex, H-Y was detected in the female; and in three species of Ranidae in which the male is heterogametic, it was detected in the male. In three species of cartilaginous fish and in one of the cyclostomes, in which heterogamety has not been ascertained, H-Y was detected in the male, suggesting that those primitive fishes are male-heterogametic. Evidently, then, heterogamety and sex-chromosome heteromorphism are polyphyletic, although certain sex-determining genes may be held in common among the diverse taxonomic groups.

scholarly journals H-Y antigens as Y chromosome-encoded gene products and serologically detectable male antigens (SDM) as testis- or spermatogenesis-linked autosomal gene products

2001 ◽  
Vol 44 (6) ◽  
pp. 677-687 ◽  
Author(s):  
S. Sutou ◽  
M. Kondo ◽  
M. Matsuda ◽  
K. Kawakura ◽  
Y. Ohinata ◽  
...  
Keyword(s):  
Disease Patient ◽  
Autosomal Gene ◽  
Transplantation Antigen ◽  
Gene Products ◽  
Protein Antigens ◽  
Expression Library ◽  
Cell Tissue ◽  
Male Specific ◽  
Determining Factor

Abstract. Male-specific transplantation antigen H-Y was proposed to be the testis-determining factor (TDF) in 1975, while SRY was found to be the TDF gene in 1990. What then of H-Y antigen? H-Y antigen was categorized into two entities, viz., T-cell mediated H-Y antigens (H-Y) and serologically detectable male antigens (SDM). Several HY genes such as Smcy, Uty and Dty have been identified and these are all Y-Iinked. H-Y is male-specific and clinically important in cell, tissue, or organ transplantations. Male-enhanced antigen 1 (Meal) was isolated from an expression library using polyclonal anti-H-Y antibody. We cloned and characterized mouse and bovine Meal/MEAI, the gene product of which was mainly localized in elongated spermatids. Mea2 was identified using monoclonal anti-H-Y antibody. MEA2 protein is localized in the Golgi apparatus of spermatocytes and spermatids. Colocalization of MEA2 protein with y-adaptin in clathrin-coated vesicles was demonstrated. Disruption of Mea2 resulted in spermatogenic failure. These findings suggest that Mea2 is involved in transportation of materials needed for acrosome components in spermatogenesis. Its human homologue is Golgin-160 which was detected in an SLE autoimmune disease patient. Müllerian inhibiting substance (MIS) was reported to have SDM activity. Taken together, SDM is a collective name for protein antigens associated with testis activity (MIS) or spermatogenesis (MEA1, MEA2) and may be antigenic when expressed in females. From the viewpoint of autoimmune diseases, the Identification and characterization of SDMs will be clinically important.

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