Inefficient gene transfer by adenovirus vector to cystic fibrosis airway epithelia of mice and humans

Nature ◽  
1994 ◽  
Vol 371 (6500) ◽  
pp. 802-806 ◽  
Author(s):  
Barbara R. Grubb ◽  
Raymond J. Pickles ◽  
Hong Ye ◽  
James R. Yankaskas ◽  
Ralph N. Vick ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Gene Transfer ◽  
Adenovirus Vector ◽  
Airway Epithelia ◽  
Cystic Fibrosis Airway

Correction of the Apical Membrane Chloride Permeability Defect in Polarized Cystic Fibrosis Airway Epithelia Following Retroviral-Mediated Gene Transfer

1992 ◽  
Vol 3 (3) ◽  
pp. 253-266 ◽  
Author(s):  
John C. Olsen ◽  
Larry G. Johnson ◽  
M. Jackson Stutts ◽  
Balazs Sarkadi ◽  
James R. Yankaskas ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Gene Transfer ◽  
Apical Membrane ◽  
Chloride Permeability ◽  
Airway Epithelia ◽  
Cystic Fibrosis Airway

scholarly journals A Chimeric Type 2 Adenovirus Vector with a Type 17 Fiber Enhances Gene Transfer to Human Airway Epithelia

1999 ◽  
Vol 73 (10) ◽  
pp. 8689-8695 ◽  
Author(s):  
Joseph Zabner ◽  
Miguel Chillon ◽  
Teresa Grunst ◽  
Thomas O. Moninger ◽  
Beverly L. Davidson ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Gene Transfer ◽  
Recombinant Adenovirus ◽  
Adenovirus Vector ◽  
Adenovirus Type ◽  
Wild Type ◽  
Airway Epithelia ◽  
Human Airway ◽  
Human Airway Epithelia

ABSTRACT In studies of the genetic disease cystic fibrosis, recombinant adenovirus type 2 (Ad2) and Ad5 are being investigated as vectors to transfer cystic fibrosis transmembrane conductance regulator cDNA to airway epithelia. However, earlier work has shown that human airway epithelia are resistant to infection by Ad2 and Ad5. Therefore, we examined the efficiency of other adenovirus serotypes at infecting airway epithelia. We found that several serotypes of adenoviruses, in particular, wild-type Ad17, infected a greater number of cells than wild-type Ad2. The increased efficiency of wild-type Ad17 could be explained by increased fiber-dependent binding to the epithelia. Therefore, we constructed a chimeric virus, Ad2(17f)/βGal-2, which is identical to Ad2/βGal-2 with the exception of having the fiber protein of Ad17 replace Ad2 fiber. This vector retained the increased binding and efficiency of gene transfer to well-differentiated human airway epithelia. These data suggest that inclusion of Ad17 fiber into adenovirus vectors may improve the outlook for gene delivery to human airway epithelia.

scholarly journals Comparison of DNA-lipid complexes and DNA alone for gene transfer to cystic fibrosis airway epithelia in vivo.

1997 ◽  
Vol 100 (6) ◽  
pp. 1529-1537 ◽  
Author(s):  
J Zabner ◽  
S H Cheng ◽  
D Meeker ◽  
J Launspach ◽  
R Balfour ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Gene Transfer ◽  
Airway Epithelia ◽  
Cystic Fibrosis Airway

In Vitro Assessment of Variables Affecting the Efficiency and Efficacy of Adenovirus-Mediated Gene Transfer to Cystic Fibrosis Airway Epithelia

1996 ◽  
Vol 7 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Larry G. Johnson ◽  
Raymond J. Pickles ◽  
Susan E. Boyles ◽  
Julia C. Morris ◽  
Hong Ye ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Gene Transfer ◽  
Airway Epithelia ◽  
Cystic Fibrosis Airway ◽  
In Vitro Assessment

scholarly journals Small-molecule ion channels increase host defences in cystic fibrosis airway epithelia

Nature ◽  
2019 ◽  
Vol 567 (7748) ◽  
pp. 405-408 ◽  
Author(s):  
Katrina A. Muraglia ◽  
Rajeev S. Chorghade ◽  
Bo Ram Kim ◽  
Xiao Xiao Tang ◽  
Viral S. Shah ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Ion Channels ◽  
Small Molecule ◽  
Airway Epithelia ◽  
Cystic Fibrosis Airway
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