Escherichia coli cell-division gene ftsZ encodes a novel GTP-binding protein

Nature ◽  
1992 ◽  
Vol 359 (6392) ◽  
pp. 251-254 ◽  
Author(s):  
Debabrata RayChaudhuri ◽  
James T. Park
Keyword(s):  
Escherichia Coli ◽  
Cell Division ◽  
Binding Protein ◽  
Coli Cell ◽  
Gtp Binding Protein ◽  
Gtp Binding

A Role for the Common GTP-Binding Protein in Coupling of Chromosome Replication to Cell Growth and Cell Division

2002 ◽  
Vol 292 (2) ◽  
pp. 333-338 ◽  
Author(s):  
Aleksandra Sikora-Borgula ◽  
Monika Słomińska ◽  
Piotr Trzonkowski ◽  
Ryszard Zielke ◽  
Andrzej Myśliwski ◽  
...  
Keyword(s):  
Cell Division ◽  
Cell Growth ◽  
Binding Protein ◽  
Chromosome Replication ◽  
Gtp Binding Protein ◽  
Gtp Binding ◽  
The Common

Impaired chromosome partitioning and synchronization of DNA replication initiation in an insertional mutant in the Vibrio harveyi cgtA gene coding for a common GTP-binding protein

2002 ◽  
Vol 362 (3) ◽  
pp. 579-584 ◽  
Author(s):  
Monika SŁOMIṄSKA ◽  
Grażyna KONOPA ◽  
Grzegorz WĘGRZYN ◽  
Agata CZYŻ
Keyword(s):  
Cell Division ◽  
Gene Product ◽  
Vibrio Harveyi ◽  
Binding Protein ◽  
Chromosome Replication ◽  
Replication Initiation ◽  
Gtp Binding Protein ◽  
Gtp Binding ◽  
Insertional Mutant ◽  
Chromosome Partitioning

The Vibrio harveyi cgtA gene product belongs to a subfamily of small GTP-binding proteins, called Obg-like proteins. Members of this subfamily are present in diverse organisms ranging from bacteria to humans. On the other hand, the functions of these proteins in the regulation of cellular processes are largely unknown. Genes coding for these proteins are essential in almost all bacteria investigated thus far. However, a viable V. harveyi insertional mutant in the cgtA gene was described recently. Therefore, this mutant gives a unique opportunity to study functions of a member of the subfamily of Obg-like proteins. Here we demonstrate that the mutant cells often form long filaments with expanded, non-partitioned or rarely partitioned chromosomes. Such a phenotype suggests impairment of the mechanism of chromosome partition. Flow cytometric studies revealed that synchronization of chromosome replication initiation is also significantly disturbed in the cgtA mutant. Moreover, in contrast to wild-type V. harveyi, inhibition of chromosome replication and/or of cell division in the mutant bacteria caused significant increase in the number of large cells, suggesting that the cgtA gene product may be involved in the coupling of cell growth to chromosome replication and cell division. These results indicate that CgtA, an Obg-like GTP-binding protein, plays an important role in the regulation of chromosomal functions.

Recruitment of the Earliest Component of the Bacterial Flagellum to the Old Cell Division Pole by a Membrane-Associated Signal Recognition Particle Family GTP-Binding Protein

2009 ◽  
Vol 391 (4) ◽  
pp. 679-690 ◽  
Author(s):  
Johnathan C.D. Green ◽  
Christina Kahramanoglou ◽  
Alamgir Rahman ◽  
Alexandra M.C. Pender ◽  
Nicolas Charbonnel ◽  
...  
Keyword(s):  
Cell Division ◽  
Binding Protein ◽  
Signal Recognition Particle ◽  
Signal Recognition ◽  
Gtp Binding Protein ◽  
Bacterial Flagellum ◽  
Gtp Binding
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