scholarly journals India blocks sale of transgenic cotton seeds

Nature ◽  
2001 ◽  
Vol 411 (6841) ◽  
pp. 983-983
Author(s):  
K. S. Jayaraman
Author(s):  
E. L. Vigil ◽  
E. F. Erbe

In cotton seeds the radicle has 12% moisture content which makes it possible to prepare freeze-fracture replicas without fixation or cryoprotection. For this study we have examined replicas of unfixed radicle tissue fractured at room temperature to obtain data on organelle and membrane structure.Excised radicles from seeds of cotton (Gossyplum hirsutum L. M-8) were fractured at room temperature along the longitudinal axis. The fracture was initiated by spliting the basal end of the excised radicle with a razor. This procedure produced a fracture through the tissue along an unknown fracture plane. The warm fractured radicle halves were placed on a thin film of 100% glycerol on a flat brass cap with fracture surface up. The cap was rapidly plunged into liquid nitrogen and transferred to a freeze- etch unit. The sample was etched for 3 min at -95°C to remove any condensed water vapor and then cooled to -150°C for platinum/carbon evaporation.


2014 ◽  
Vol 39 (2) ◽  
pp. 317-326 ◽  
Author(s):  
C. Márquez-Hernández ◽  
S. Santana ◽  
V. Ávila ◽  
J.L. García ◽  
P. Preciado ◽  
...  
Keyword(s):  

1986 ◽  
Vol 22 (6) ◽  
pp. 635-642
Author(s):  
S. D. Gusakova ◽  
S. G. Yunusova ◽  
T. V. Chernenko ◽  
I. P. Nazarova ◽  
A. I. Glushenkova
Keyword(s):  

1974 ◽  
Vol 10 (6) ◽  
pp. 785-787
Author(s):  
N. R. Dzhanbaeva ◽  
M. M. Rakhimov ◽  
P. Kh. Yuldashev

2016 ◽  
Vol 23 (3) ◽  
pp. 358-362 ◽  
Author(s):  
Sayed Sartaj Sohrab ◽  
Mohammad A. Kamal ◽  
Abdul Ilah ◽  
Azamal Husen ◽  
P.S. Bhattacharya ◽  
...  

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
M. K. Daud ◽  
Lei Mei ◽  
M. T. Variath ◽  
Shafaqat Ali ◽  
Cheng Li ◽  
...  

Chromium (Cr) is present in our environment as a toxic pollutant, which needs to be removed using phytoremediation technology. In present study, two transgenic cotton cultivars (J208, Z905) and their hybrid line (ZD14) were used to explore their Cr uptake and tolerance potential using multiple biomarkers approach. Four different levels of Cr (CK, 10, 50, and 100 μM) were applied. Cr caused a significant reduction in root/shoot length, number of secondary roots, and root fresh and dry biomasses at 100μM. Cr accumulated more in roots and was found higher in hybrid line (ZD14) as compared with its parent lines (J208, Z905) at all Cr stress levels (10, 50, and 100 μM). Cr translocation was less than 1 in all cultivars. Ultrastructural studies at 100 μM Cr showed an increase in number of nuclei and vacuoles and presence of Cr dense granules in dead parts of the cell (vacuoles/cell wall). Malondialdehyde (MDA), hydrogen peroxide (H2O2), total soluble proteins, superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), and glutathione reductase (GR) as a whole were upregulated with elevated levels of Cr. Higher Cr uptake by roots, accelerated metabolism, and Cr sequestration in dead parts of the cell indicate that these cotton cultivars can be useful for Cr accumulation and tolerance.


2014 ◽  
Vol 33 (2) ◽  
pp. 167-177 ◽  
Author(s):  
Guoxin Shen ◽  
Jia Wei ◽  
Xiaoyun Qiu ◽  
Rongbin Hu ◽  
Sundaram Kuppu ◽  
...  

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