High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor

Nature ◽  
1991 ◽  
Vol 350 (6320) ◽  
pp. 678-683 ◽  
Author(s):  
Barbara L. Hempstead ◽  
Dionisio Martin-Zanca ◽  
David R. Kaplan ◽  
Luis F. Parada ◽  
Moses V. Chao
Keyword(s):  
High Affinity ◽  
Ngf Receptor

scholarly journals Expression of β‐NGF and high‐affinity NGF receptor (TrKA) in llama ( Lama glama ) male reproductive tract and spermatozoa

2018 ◽  
Vol 85 (12) ◽  
pp. 934-944 ◽  
Author(s):  
Luciana M. Sari ◽  
Renato Zampini ◽  
Martin E. Argañaraz ◽  
María I. Carretero ◽  
Fernanda G. Fumuso ◽  
...  
Keyword(s):  
Reproductive Tract ◽  
Male Reproductive Tract ◽  
High Affinity ◽  
Ngf Receptor ◽  
Lama Glama

Chapter 2 Axonal transport of the trkA high-affinity NGF receptor

1994 ◽  
pp. 15-21 ◽  
Author(s):  
Alonzo H. Ross ◽  
Mahesh B. Lachyankar ◽  
Dorota K. Poluha ◽  
Rebekah Loy
Keyword(s):  
Axonal Transport ◽  
High Affinity ◽  
Ngf Receptor

Expression of nerve growth factor and neurotrophin receptors in testicular cells suggest novel roles for neurotrophins outside the nervous system

1996 ◽  
Vol 8 (7) ◽  
pp. 1075 ◽  
Author(s):  
K Seidl ◽  
A Buchberger ◽  
C Erck
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Leydig Cells ◽  
Expression Patterns ◽  
Myoid Cells ◽  
Nerve Growth ◽  
High Affinity ◽  
Ngf Receptor

The present study was designed to clarify the non-neurotrophic role for neurotrophins in mouse testis. By means of SI nuclease protection assay we could demonstrate that the gene coding for the low-affinity nerve growth factor (NGF) receptor p75NGFR is transiently expressed during germ cell development. Gene expression for p75NGFR was detected in late-meiotic spermatocytes and early spermatids and was found to be co-expressed with trkB and trkC, two tyrosine kinase receptors, commonly regarded as the high-affinity receptors for brain-derived neurotrophic factor and neurotrophin-3. Gene transcripts for the high-affinity NGF receptor trkA were found exclusively in non-germ cells. Isolated Leydig cells, peritubular myoid cells and Sertoli cells, but not germ cells, could be identified as potential testicular NGF sources. Non-germ cells respond after incubation for several days with a sharp induction in NGF synthesis, which is accompanied by a loss of phenotypic expression patterns. The fact that p75NGFR mRNA expression was induced in cultured Sertoli cells and peritubular myoid cells suggests an autocrine mode of NGF action in these cells. Induction of NGF synthesis in cultured Leydig cells could be prevented by the glucocorticoid dexamethasone. Results indicate different roles for the individual neurotrophins in distinct testicular compartments and suggest that these neurotrophins might support testicular functions by signalling between individual cell types in an autocrine and paracrine manner.

scholarly journals Intracelluar Dynamics of a High Affinity NGF Receptor TrkA in PC12 Cell.

2000 ◽  
Vol 23 (9) ◽  
pp. 1097-1099 ◽  
Author(s):  
Naohide HIRASHIMA ◽  
Masashi NISHINO ◽  
Mamoru NAKANISHI
Keyword(s):  
Pc12 Cell ◽  
High Affinity ◽  
Ngf Receptor

Postnatal Changes in the Expression of the trkA High-affinity NGF Receptor in Primary Sensory Neurons

1996 ◽  
Vol 8 (10) ◽  
pp. 2204-2208 ◽  
Author(s):  
David L. H. Ennett ◽  
Sharon Averill ◽  
Douglas O. Clary ◽  
John V. Priestley ◽  
Stephen B. McMahon
Keyword(s):  
Sensory Neurons ◽  
Primary Sensory Neurons ◽  
High Affinity ◽  
Ngf Receptor

Glycation of the high affinity NGF-receptor and RAGE leads to reduced ligand affinity

2015 ◽  
Vol 150 ◽  
pp. 1-11 ◽  
Author(s):  
Dorit Bennmann ◽  
Christoph Kannicht ◽  
Claudine Fisseau ◽  
Kathleen Jacobs ◽  
Alexander Navarette-Santos ◽  
...  
Keyword(s):  
Ligand Affinity ◽  
High Affinity ◽  
Ngf Receptor

scholarly journals PC12 cell mutants that possess low- but not high-affinity nerve growth factor receptors neither respond to nor internalize nerve growth factor.

1986 ◽  
Vol 102 (3) ◽  
pp. 830-843 ◽  
Author(s):  
S H Green ◽  
R E Rydel ◽  
J L Connolly ◽  
L A Greene
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cell Surface ◽  
Pc12 Cells ◽  
Selection Procedure ◽  
Division Rate ◽  
Nerve Growth ◽  
High Affinity ◽  
Ngf Receptor ◽  
Epidermal Growth

Four mutant PC12 pheochromocytoma cell lines that are nerve growth factor (NGF)-nonresponsive (PC12nnr) have been selected from chemically mutagenized cultures by a double selection procedure: failure both to grow neurites in the presence of NGF and to survive in NGF-supplemented serum-free medium. The PC12nnr cells were deficient in all additional NGF responses surveyed: abatement of cell proliferation, changes in glycoprotein composition, induction of ornithine decarboxylase, rapid changes in protein phosphorylation, and cell surface ruffling. However, PC12nnr cells closely resembled non-NGF-treated PC12 cells in most properties tested: cell size and shape; division rate; protein, phosphoprotein, and glycoprotein composition; and cell surface morphology. All four PC12nnr lines differed from PC12 cells in three ways in addition to failure of NGF response: PC12nnr cells failed to internalize bound NGF by the normal, saturable, high-affinity mechanism present in PC12 cells. The PC12nnr cells bound NGF but entirely, or nearly entirely, at low-affinity sites only, whereas PC12 cells possess both high- and low-affinity NGF binding sites. The responses to dibutyryl cyclic AMP that were tested appeared to be enhanced or altered in the PC12nnr cells compared to PC12 cells. Internalization of, and responses to, epidermal growth factor were normal in the PC12nnr cells ruling out a generalized defect in hormonal binding, uptake, or response mechanisms. These findings are consistent with a causal association between the presence of high-affinity NGF receptors and of NGF responsiveness and internalization. A possible relationship is also suggested between regulation of cAMP responses and regulation of NGF responses or NGF receptor affinity.

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