scholarly journals Functional annotation of a full-length mouse cDNA collection

Nature ◽  
2001 ◽  
Vol 409 (6821) ◽  
pp. 685-690 ◽  
Author(s):  
Keyword(s):  
Functional Annotation ◽  
Full Length ◽  
Mouse Cdna ◽  
Cdna Collection

Isolation and characterization of full-length mouse cDNA and genomic clones of 3-methylcholanthrene-inducible cytochrome P1-450 and P3-450

Gene ◽  
1984 ◽  
Vol 29 (3) ◽  
pp. 281-292 ◽  
Author(s):  
Frank J. Gonzalez ◽  
Peter I. Mackenzie ◽  
Shioko Kimura ◽  
Daniel W. Nebert
Keyword(s):  
Full Length ◽  
Genomic Clones ◽  
Mouse Cdna ◽  
Isolation And Characterization

scholarly journals Functional annotation of 19,841 Populus nigra full-length enriched cDNA clones

BMC Genomics ◽  
2007 ◽  
Vol 8 (1) ◽  
pp. 448 ◽  
Author(s):  
Tokihiko Nanjo ◽  
Tetsuya Sakurai ◽  
Yasushi Totoki ◽  
Atsushi Toyoda ◽  
Mitsuru Nishiguchi ◽  
...  
Keyword(s):  
Functional Annotation ◽  
Populus Nigra ◽  
Full Length ◽  
Cdna Clones

scholarly journals Establishing a System for Functional Characterization of Full-Length cDNAs of Camellia sinensis

2019 ◽  
Vol 20 (23) ◽  
pp. 5929
Author(s):  
Lin ◽  
Cai ◽  
Du ◽  
Zhang ◽  
Xu ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Heterologous Expression ◽  
Cdna Library ◽  
Camellia Sinensis ◽  
Functional Characterization ◽  
Full Length ◽  
Rare Gene ◽  
Cdna Collection ◽  
Reference Genomes

: Tea (Camellia sinensis) is enriched with bioactive secondary metabolites, and is one of the most popular nonalcoholic beverages globally. Two tea reference genomes have been reported; however, the functional analysis of tea genes has lagged, mainly due to tea’s recalcitrance to genetic transformation and the absence of alternative high throughput heterologous expression systems. A full-length cDNA collection with a streamlined cloning system is needed in this economically important woody crop species. RNAs were isolated from nine different vegetative tea tissues, pooled, then used to construct a normalized full-length cDNA library. The titer of unamplified and amplified cDNA library was 6.89 × 106 and 1.8 × 1010 cfu/mL, respectively; the library recombinant rate was 87.2%. Preliminary characterization demonstrated that this collection can complement existing tea reference genomes and facilitate rare gene discovery. In addition, to streamline tea cDNA cloning and functional analysis, a binary vector (pBIG2113SF) was reengineered, seven tea cDNAs isolated from this library were successfully cloned into this vector, then transformed into Arabidopsis. One FL-cDNA, which encodes a putative P1B-type ATPase 5 (CsHMA5), was characterized further as a proof of concept. We demonstrated that overexpression of CsHMA5 in Arabidopsis resulted in copper hyposensitivity. Thus, our data demonstrated that this represents an efficient system for rare gene discovery and functional characterization of tea genes. The integration of a tea FL-cDNA collection with efficient cloning and a heterologous expression system would facilitate functional annotation and characterization of tea genes.

scholarly journals Cloning and expression analysis of full length mouse cDNA sequences encoding the transformation associated protein p53

1984 ◽  
Vol 12 (14) ◽  
pp. 5609-5626 ◽  
Author(s):  
John R. Jenkins ◽  
Karran Rudge ◽  
Shelagh Redmond ◽  
Alison Wade-Evans
Keyword(s):  
Expression Analysis ◽  
Full Length ◽  
Cloning And Expression ◽  
Cdna Sequences ◽  
Mouse Cdna ◽  
Protein P53
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