Embryonic expression of a haematopoietic growth factor encoded by the SI locus and the ligand for c-kit

Nature ◽  
1990 ◽  
Vol 347 (6294) ◽  
pp. 667-669 ◽  
Author(s):  
Yasuhisa Matsui ◽  
Kristina M. Zsebo ◽  
Brigid L. M. Hogan
Keyword(s):  
Growth Factor ◽  
Haematopoietic Growth Factor

Mammalian cell production and purification of progenipoietin, a dual-agonist chimaeric haematopoietic growth factor

2003 ◽  
Vol 37 (1) ◽  
pp. 31 ◽  
Author(s):  
David C. Wood ◽  
Karl J. Mathis ◽  
William D. Joy ◽  
John C. Minnerly ◽  
Lyle E. Pegg ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mammalian Cell ◽  
Cell Production ◽  
Haematopoietic Growth Factor ◽  
Dual Agonist

Abstract 549: Extracellular Heat Shock Protein 27 Signals Through NF-κB to Favorably Modulate Macrophage Inflammation

2012 ◽  
Vol 32 (suppl_1) ◽  
Author(s):  
Tara Seibert ◽  
Chunhua Shi ◽  
Yong-Xiang Chen ◽  
Samira Salari ◽  
Joshua Raizman ◽  
...  
Keyword(s):  
Growth Factor ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Reporter Gene ◽  
Nuclear Translocation ◽  
Atherosclerotic Lesion ◽  
Heat Shock Protein 27 ◽  
Therapeutic Effects ◽  
Gm Csf ◽  
Haematopoietic Growth Factor

Background: Previously we demonstrated that recombinant Heat Shock Protein 27 (rHSP27) reduces atherosclerotic lesion formation when administered to ApoE -/- mice. In addition, administration of rHSP27 to ApoE -/- mice with established atherosclerotic lesions halts lesion progression and is associated with lesion modifications that are consistent with resilience to plaque rupture. However, the mechanism(s) for these therapeutic effects remain elusive. Objective: To determine whether rHSP27 favorably modulates macrophage inflammation by focusing on NF-kB signaling. Methods/Results: Activation of the NF-kB signaling pathway by rHSP27 was observed in peritoneal macrophages from ApoE -/- mice. Treatment with rHSP27 for 30 minutes activated the translocation of the NF-kB p65 subunit from the cytosol to the nucleus as observed by immunolabeling. A dose-dependant increase in rHSP27 mediated NF-kB activation was observed in RAW 264.7 macrophages stably transfected with an NF-kB inducible reporter gene (15 fold; p<0.05). The use of an N-terminal deletion mutant of rHSP27, rC1, at equimolar concentrations did not induce NF-kB activation, demonstrating specificity of the full-length protein. In addition, NF-kB inhibitors (BAY 11-7082 and MG-132) attenuated the induction of the NF-kB reporter gene by rHSP27, thereby implicating the involvement of IkBa phosphorylation and degradation by the proteasome. A consequence of rHSP27 signaling in macrophages was the up-regulation of the transcript for the haematopoietic growth factor/regulator, GM-CSF (300 fold; p<0.05) and subsequently its secretion (400 fold, p<0.05). In the presence of BAY, GM-CSF expression was inhibited suggesting the involvement of NF-kB. Conclusions: rHSP27 promotes the nuclear translocation and activation of NF-kB in macrophages which results in the up-regulation of GM-CSF, a haematopoietic growth factor/regulator that may be responsible for the observed therapeutic effects of rHSP27 in vivo. Current studies are testing rHSP27 therapy in ApoE -/- mice deficient in GM-CSF in order to evaluate the importance of macrophage modulation for the beneficial affects of rHSP27 in atherogenesis.

Granulocyte–macrophage colony-stimulating factor: not just another haematopoietic growth factor

2013 ◽  
Vol 31 (1) ◽  
Author(s):  
Alejandro Francisco-Cruz ◽  
Miguel Aguilar-Santelises ◽  
Octavio Ramos-Espinosa ◽  
Dulce Mata-Espinosa ◽  
Brenda Marquina-Castillo ◽  
...  
Keyword(s):  
Growth Factor ◽  
Colony Stimulating Factor ◽  
Haematopoietic Growth Factor ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

Compartmentalization of a haematopoietic growth factor (GM-CSF) by glycosaminoglycans in the bone marrow microenvironment

Nature ◽  
1987 ◽  
Vol 326 (6111) ◽  
pp. 403-405 ◽  
Author(s):  
Myrtle Y. Gordon ◽  
Graham P. Riley ◽  
Suzanne M. Watt ◽  
Melvyn F. Greaves
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Bone Marrow Microenvironment ◽  
Gm Csf ◽  
Haematopoietic Growth Factor
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