A copia-like transposable element family in Arabidopsis thaliana

Nature ◽  
1988 ◽  
Vol 336 (6196) ◽  
pp. 242-244 ◽  
Author(s):  
Daniel F. Voytas ◽  
Frederick M. Ausubel
Keyword(s):  
Arabidopsis Thaliana ◽  
Transposable Element ◽  
Transposable Element Family

scholarly journals Arabidopsis MORC proteins function in the efficient establishment of RNA directed DNA methylation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yan Xue ◽  
Zhenhui Zhong ◽  
C. Jake Harris ◽  
Javier Gallego-Bartolomé ◽  
Ming Wang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Arabidopsis Thaliana ◽  
Transposable Element ◽  
Zinc Finger ◽  
Protein Function ◽  
C Elegans ◽  
Eukaryotic Organisms

AbstractThe Microrchidia (MORC) family of ATPases are required for transposable element (TE) silencing and heterochromatin condensation in plants and animals, and C. elegans MORC-1 has been shown to topologically entrap and condense DNA. In Arabidopsis thaliana, mutation of MORCs has been shown to reactivate silent methylated genes and transposons and to decondense heterochromatic chromocenters, despite only minor changes in the maintenance of DNA methylation. Here we provide the first evidence localizing Arabidopsis MORC proteins to specific regions of chromatin and find that MORC4 and MORC7 are closely co-localized with sites of RNA-directed DNA methylation (RdDM). We further show that MORC7, when tethered to DNA by an artificial zinc finger, can facilitate the establishment of RdDM. Finally, we show that MORCs are required for the efficient RdDM mediated establishment of DNA methylation and silencing of a newly integrated FWA transgene, even though morc mutations have no effect on the maintenance of preexisting methylation at the endogenous FWA gene. We propose that MORCs function as a molecular tether in RdDM complexes to reinforce RdDM activity for methylation establishment. These findings have implications for MORC protein function in a variety of other eukaryotic organisms.

scholarly journals Characterization of the Putative Transposase mRNA of Tag1, Which Is Ubiquitously Expressed in Arabidopsis and Can Be Induced by Agrobacterium-Mediated Transformation With dTag1 DNA

Genetics ◽  
1998 ◽  
Vol 149 (2) ◽  
pp. 693-701 ◽  
Author(s):  
Dong Liu ◽  
Nigel M Crawford
Keyword(s):  
Arabidopsis Thaliana ◽  
Transposable Element ◽  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Transcript Levels ◽  
Agrobacterium Mediated Transformation ◽  
Transgenic Lines ◽  
Localization Signal ◽  
Transposase Mrna

Abstract Tag1 is an autonomous transposable element of Arabidopsis thaliana. Tag1 expression was examined in two ecotypes of Arabidopsis (Columbia and No-0) that were transformed with CaMV 35S-Tag1-GUS DNA. These ecotypes contain no endogenous Tag1 elements. A major 2.3-kb and several minor transcripts were detected in all major organs of the plants. The major transcript encoded a putative transposase of 84.2 kD with two nuclear localization signal sequences and a region conserved among transposases of the Ac or hAT family of elements. The abundance of Tag1 transcripts varied among transgenic lines and did not correlate with somatic excision frequency or germinal reversion rates, suggesting that factors other than transcript levels control Tag1 excision activity. In untransformed plants of the Landsberg ecotype, which contain two endogenous Tag1 elements, no Tag1 transcripts were detected. Agrobacterium-mediated transformation of these Landsberg plants with a defective 1.4-kb Tag1 element resulted in the appearance of full-length Tag1 transcripts from the endogenous elements. Transformation with control DNA containing no Tag1 sequences did not activate endogenous Tag1 expression. These results indicate that Agrobacterium-mediated transformation with dTag1 can activate the expression of Tag1.

scholarly journals Behaviour of the maize transposable element Ac in Arabidopsis thaliana

1992 ◽  
Vol 2 (1) ◽  
pp. 69-81 ◽  
Author(s):  
Caroline Dean ◽  
Christina Sjodin ◽  
Tania Page ◽  
Jonathan Jones ◽  
Clare Lister
Keyword(s):  
Arabidopsis Thaliana ◽  
Transposable Element ◽  
Maize Transposable Element

scholarly journals Molecular evolution of the Ac/Ds transposable-element family in pearl millet and other grasses.

Genetics ◽  
1995 ◽  
Vol 139 (3) ◽  
pp. 1411-1419
Author(s):  
G A Huttley ◽  
A F MacRae ◽  
M T Clegg
Keyword(s):  
Transposable Element ◽  
Pearl Millet ◽  
Pennisetum Glaucum ◽  
Grass Species ◽  
Deletion Derivative ◽  
Transposable Element Family ◽  
Sequence Identity ◽  
Grass Family ◽  
Sequence Relationships ◽  
Different Levels

Abstract We report an Ac-like sequence from pearl millet (Pennisetum glaucum) and deletion derivative Ac-like sequences from pearl millet and another grass species, Bambusa multiplex. Sequence relationships between the pearl millet and maize Ac elements suggest that Ac/Ds transposable-element family is ancient. Further, the sequence identity between the Bambusa Ac-like sequence and maize Ac implies that the Ac/Ds transposable-element family has been in the grass family since its inception. The Ac-like sequences reported from pearl millet and maize Ac are statistically heterogeneous in pair-wise distance comparisons to each other. Yet, we are unable to discriminate between differential selection or ectopic exchange (recombination and conversion) between nonidentical transposable element homologues, as the cause of the heterogeneity. However, the more extreme heterogeneity exhibited between the previously described pearl millet element and maize Ac seems likely to derive from ectopic exchange between elements with different levels of divergence.

scholarly journals Expansion of a single transposable element family is associated with genome-size increase and radiation in the genus Hydra

2019 ◽  
Vol 116 (46) ◽  
pp. 22915-22917 ◽  
Author(s):  
Wai Yee Wong ◽  
Oleg Simakov ◽  
Diane M. Bridge ◽  
Paulyn Cartwright ◽  
Anthony J. Bellantuono ◽  
...  
Keyword(s):  
Transposable Elements ◽  
Transposable Element ◽  
Genome Size ◽  
Focal Point ◽  
Size Increase ◽  
Single Family ◽  
Evolutionary Patterns ◽  
Transposable Element Family ◽  
Chicken Repeat ◽  
Long Interspersed Nuclear Elements

Transposable elements are one of the major contributors to genome-size differences in metazoans. Despite this, relatively little is known about the evolutionary patterns of element expansions and the element families involved. Here we report a broad genomic sampling within the genus Hydra, a freshwater cnidarian at the focal point of diverse research in regeneration, symbiosis, biogeography, and aging. We find that the genome of Hydra is the result of an expansion event involving long interspersed nuclear elements and in particular a single family of the chicken repeat 1 (CR1) class. This expansion is unique to a subgroup of the genus Hydra, the brown hydras, and is absent in the green hydra, which has a repeat landscape similar to that of other cnidarians. These features of the genome make Hydra attractive for studies of transposon-driven genome expansions and speciation.

Sign in / Sign up

Export Citation Format

Share Document