Differential regulation of PI hydrolysis and adenylyl cyclase by muscarinic receptor subtypes

Nature ◽  
1988 ◽  
Vol 334 (6181) ◽  
pp. 434-437 ◽  
Author(s):  
Ernest G. Peralta ◽  
Avi Ashkenazi ◽  
John W. Winslow ◽  
J. Ramachandran ◽  
Daniel J. Capon
Keyword(s):  
Adenylyl Cyclase ◽  
Muscarinic Receptor ◽  
Differential Regulation ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes ◽  
Pi Hydrolysis

Expression of muscarinic receptor subtypes and M2-muscarinic inhibition of adenylyl cyclase in lung

1995 ◽  
Vol 268 (1) ◽  
pp. L101-L107 ◽  
Author(s):  
C. W. Emala ◽  
A. Aryana ◽  
M. A. Levine ◽  
R. P. Yasuda ◽  
S. A. Satkus ◽  
...  
Keyword(s):  
Adenylyl Cyclase ◽  
Muscarinic Receptor ◽  
Muscarinic Receptors ◽  
Lung Parenchyma ◽  
Functional Coupling ◽  
Receptor Subtypes ◽  
Relative Distribution ◽  
Muscarinic Receptor Subtypes ◽  
M3 Receptors ◽  
Trachealis Muscle

The relative distribution and absolute quantities of muscarinic receptor subtypes m1, m2, m3, and m4 were determined in membranes of canine trachealis muscle, bronchi, and lung parenchyma by immuno-precipitation with receptor subtype-specific rabbit polyclonal antisera. Additionally, the functional coupling of muscarinic receptors to the inhibition of adenylyl cyclase was related to the presence of m2-muscarinic receptors in each region. Immunoprecipitation identified more total muscarinic receptors in trachealis muscle than in bronchi or lung. m2-Muscarinic receptor predominated in tracheal muscle (372 +/- 85 fmol/mg protein) with fewer m3 receptors (48 +/- 5 fmol/mg protein). Bronchi contained 6.6 +/- 2.0 and 9.2 +/- 1.8 fmol/mg protein of m2 and m3 receptors, respectively. Lung parenchyma contained 13.9 +/- 3.9 fmol/mg protein of m3 receptors. Adenylyl cyclase activity increased in response to guanosine triphosphate and isoproterenol in membranes from all three lung regions, but muscarinic-mediated inhibition of adenylyl cyclase occurred only in trachealis membranes. These studies provide the first quantitative assessment of muscarinic receptor subtypes in different regions of the lung and relate the ability to measure muscarinic inhibition of adenylyl cyclase to the presence of m2 receptors.

In Vivo Characterization of Muscarinic Receptor Subtypes That Mediate Vasodilatation in Patients With Essential Hypertension

Hypertension ◽  
1995 ◽  
Vol 26 (1) ◽  
pp. 70-77 ◽  
Author(s):  
Tobias A. Bruning ◽  
Peter C. Chang ◽  
Maarten G.C. Hendriks ◽  
Pieter Vermeij ◽  
Martin Pfaffendorf ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Muscarinic Receptor ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes ◽  
In Vivo Characterization

Pre- and postjunctional muscarinic receptor subtypes in the vas deferens of rat

1994 ◽  
Vol 25 (8) ◽  
pp. 1643-1647 ◽  
Author(s):  
H.F. Miranda ◽  
E. Duran ◽  
D. Bustamante ◽  
C. Paeile ◽  
G. Pinardi
Keyword(s):  
Muscarinic Receptor ◽  
Vas Deferens ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes
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