Virus resistance in transgenic plants that express cucumber mosaic virus satellite RNA

Nature ◽  
1987 ◽  
Vol 328 (6133) ◽  
pp. 799-802 ◽  
Author(s):  
Bryan D. Harrison ◽  
Michael A. Mayo ◽  
David C. Baulcombe
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Resistance ◽  
Satellite Rna

scholarly journals Transgenic Tomato Plants Expressing Satellite RNA Are Tolerant to Some Strains of Cucumber Mosaic Virus

1994 ◽  
Vol 119 (3) ◽  
pp. 642-647 ◽  
Author(s):  
P.B. McGarvey ◽  
M.S. Montasser ◽  
J.M. Kaper
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Unit Length ◽  
Transgenic Tomato ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Satellite Rna ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

Transgenic tomato plants (Lycopersicon esculentum Mill.) expressing cucumber mosaic virus (CMV) satellite RNA fused to a gene for β-glucuronidase were produced using Agrobacterium-mediated transformation. The R1 progeny of self-crossed R0 plants were challenge-inoculated with virion or RNA preparations of CMV or tomato aspermy virus (TAV). The transgenic plants challenged with CMV-1 showed mild disease symptoms in the first 2 weeks postchallenge followed by a decrease in symptoms, resulting in little difference between the transgenic and uninfected control group by the fourth week. Enzyme-linked immunosorbent assay results showed about a 10-fold decrease in virus accumulation in the transgenic plants compared to controls. Tolerance was evident only in plants that contained the recombinant insert and produced mature unit-length satellite RNA after CMV infection. Plants challenged with TAV showed no significant tolerance to virus-induced symptoms.

scholarly journals Protective Effect of a Satellite RNA Expressed in Transgenic Plants on Disease Incidence after Inoculation of Cucumber Mosaic Virus.

1994 ◽  
Vol 60 (2) ◽  
pp. 228-232 ◽  
Author(s):  
Chikara MASUTA ◽  
Yumiko HAYASHI ◽  
Masashi SUZUKI ◽  
Shigeru KUWATA ◽  
Yoichi TAKANAMI ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Protective Effect ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Disease Incidence ◽  
Satellite Rna

scholarly journals Delay of Disease Development in Transgenic Petunia Plants Expressing Cucumber Mosaic Virus I17N-Satellite RNA

1995 ◽  
Vol 120 (2) ◽  
pp. 353-359 ◽  
Author(s):  
Shin Je Kim ◽  
Kyung-Hee Paek ◽  
Byung-Dong Kim
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Unit Length ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Disease Development ◽  
Satellite Rna ◽  
Camv 35S ◽  
Transgenic Petunia

A cDNA clone of cucumber mosaic virus (CMV) 117 N-satellite RNA driven by the cauliflower mosaic virus (CaMV) 35S transcript promoter, was stably integrated into the genome of Petunia hybrida `Bluepicoti' tissues by Agrobacterium tumefaciens Ti plasmid-mediated transformation. Transgenic plants producing CMV satellite RNA showed delayed disease development when inoculated with CMV-Y, a helper virus for the I17N-satellite RNA. Furthermore, transgenic petunia plants showed delayed disease development against tobacco mosaic virus (TMV), a tobamovirus not related to CMV. Northern blot analysis revealed that large amounts of unit length satellite RNA (335 bp) were produced in CMV-infected transgenic petunia plants; whereas, mainly transcripts driven by the CaMV 35S promoter (approximately 1 kb) were produced in TMV-infected transgenic plants. SDS-PAGE and Western blotting showed that symptom reduction was correlated with a reduction in the amount of viral coat protein in transgenic plants.

scholarly journals Analysis of Mechanisms Involved in the Cucumber mosaic virus Satellite RNA-mediated Transgenic Resistance in Tomato Plants

2004 ◽  
Vol 17 (1) ◽  
pp. 98-108 ◽  
Author(s):  
Fabrizio Cillo ◽  
Mariella M. Finetti-Sialer ◽  
Maria A. Papanice ◽  
Donato Gallitelli
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Resistance Mechanism ◽  
Degradation Process ◽  
Transgenic Tomato ◽  
Satellite Rna ◽  
Total Recovery ◽  
Tomato Plants ◽  
Free Strain

Transgenic tomato (Lycopersicon esculentum Mill. cv. UC82) plants expressing a benign variant of Cucumber mosaic virus satellite RNA (CMV Tfn-satRNA) were generated. The transformed plants did not produce symptoms when challenged with a satRNA-free strain of CMV (CMV-FL). The same plant lines initially were susceptible to necrosis elicited by a CMV strain supporting a necrogenic variant of satRNA (CMV-77), but a phenotype of total recovery from the necrosis was observed in the newly developing leaves. The features of the observed resistance were analyzed and are consistent with two different mechanisms of resistance. In transgenic plants inoculated with CMV-FL strain, the symptomless phenotype was correlated to the down-regulation of CMV by Tfn-satRNA, amplified from the transgene transcripts, as the first resistance mechanism. On the other hand, the delayed resistance to CMV-77 in transgenic tomato lines was mediated by a degradation process that targets satRNAs in a sequence-specific manner. Evidence is provided for a correlation between a reduced accumulation level of transgenic messenger Tfn-satRNA, the accumulation of small (approximately 23 nucleotides) RNAs with sequence homology to satRNAs, the progressively reduced accumulation of 77-satRNA in infected tissues, and the transition in infected plants from diseased to healthy. Thus, events leading to the degradation of satRNA sequences indicate a role for RNA silencing as the second mechanism determining resistance of transgenic tomato lines.

Sign in / Sign up

Export Citation Format

Share Document