Protein kinase C injection into hippocampal pyramidal cells elicits features of long term potentiation

Nature ◽  
1987 ◽  
Vol 328 (6129) ◽  
pp. 426-429 ◽  
Author(s):  
G.-Y. Hu ◽  
Ø. Hvalby ◽  
S. I. Walaas ◽  
K. A. Albert ◽  
P. Skjeflo ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Pyramidal Cells ◽  
Long Term Potentiation ◽  
Kinase C ◽  
Term Potentiation ◽  
Hippocampal Pyramidal Cells

Bifemelane induces translocation of protein kinase C in the CA3, but not the CA1, region of guinea-pig hippocampus

1990 ◽  
Vol 68 (3) ◽  
pp. 413-418 ◽  
Author(s):  
Takeshi Fujii ◽  
Yasushi Kuraishi ◽  
Toshikazu Okada ◽  
Masamichi Satoh
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Guinea Pig ◽  
Long Term Potentiation ◽  
Binding Activity ◽  
Ca1 Region ◽  
Kinase C ◽  
Term Potentiation ◽  
Ca3 Region

We made use of the [3H]phorbol 12,13-dibutyrate binding assay to investigate the effects of bifemelane on the subcellular distribution of protein kinase C in the CA3 and CA1 regions of guinea-pig hippocampal slices. Bifemelane, a drug that augments the long-term potentiation in the CA3 region, significantly induced the translocation of [3H]phorbol 12,13-dibutyrate binding activity from the cytosol to the membrane in a dose-dependent manner (10−8 to 10−6 M) and with no effects on total binding activity in the CA3 region. Bifemelane, at a concentration of 10−6 M, was without effect on the subcellular distribution of [3H]phorbol 12,13-dibutyrate binding activity in the CA1 region. These observations suggest that bifemelane acts directly on the hippocampus to induce translocation of protein kinase C in the CA3 region. Such an effect may be associated with the bifemelane-induced augmentation of the long-term potentiation in this region of the brain.Key words: bifemelane, protein kinase C, hippocampal slice, translocation, CA3.

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