Pulsed-field gel electrophoresis of large DNA molecules

Nature ◽  
1986 ◽  
Vol 319 (6055) ◽  
pp. 701-702 ◽  
Author(s):  
Cassandra L. Smith ◽  
Charles R. Cantor
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Dna Molecules ◽  
Pulsed Field

Fast Procedure To Distinguish Circular and Linear DNA Molecules in Pulsed Field Gel Electrophoresis

1996 ◽  
Vol 29 (5) ◽  
pp. 745-753 ◽  
Author(s):  
M. Báez-Camargo ◽  
L. Lopez-Canovas ◽  
A. M. Riveron ◽  
P. Chávez ◽  
E. Orozco
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Dna Molecules ◽  
Pulsed Field ◽  
Linear Dna ◽  
Fast Procedure

scholarly journals Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae

1998 ◽  
Vol 21 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Maria Cléria Valadares-Inglis ◽  
John F. Peberdy
Keyword(s):  
Genome Size ◽  
Metarhizium Anisopliae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
The Other ◽  
Electrophoretic Karyotype ◽  
Dna Molecules ◽  
Chromosomal Dna ◽  
Pulsed Field ◽  
Densitometric Analysis

Pulsed-field gel electrophoresis (PFGE) was used to separate chromosome-sized DNA molecules of four strains of Metarhizium anisopliae from Brazil. Metarhizium anisopliae isolates from Japan have been reported as possessing seven chromosomes. Variation was observed among the Brazilian strains and the chromosomal DNA was resolved into eight bands for strain CG46. Densitometric analysis of PFGE gels suggested that the other three Brazilian strains also possess eight chromosomes, with two chromosomes migrating as doublets under the electrophoretic conditions used. The genome size was estimated as varying between 23.39 to 31.88 Mb, not including possible doublet chromosomes.

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