A short conserved sequence is involved in the light-inducibility of a gene encoding ribulose 1,5-bisphosphate carboxylase small subunit of pea

Nature ◽  
1985 ◽  
Vol 315 (6016) ◽  
pp. 200-204 ◽  
Author(s):  
Giorgio Morelli ◽  
Ferenc Nagy ◽  
Robert T. Fraley ◽  
Stephen G. Rogers ◽  
Nam-Hai Chua
Keyword(s):  
Small Subunit ◽  
Gene Encoding ◽  
Bisphosphate Carboxylase ◽  
Conserved Sequence ◽  
Light Inducibility

scholarly journals Transcriptional regulation of a gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean tissue is linked to the phytochrome response.

1985 ◽  
Vol 5 (8) ◽  
pp. 1910-1917 ◽  
Author(s):  
S L Berry-Lowe ◽  
R B Meagher
Keyword(s):  
Ribosomal Rna ◽  
White Light ◽  
Red Light ◽  
Small Subunit ◽  
Subunit Gene ◽  
Gene Encoding ◽  
Bisphosphate Carboxylase ◽  
Subunit Mrna ◽  
Etiolated Seedlings ◽  
Soybean Tissue

The effects of white light, far-red light, and darkness on the transcription of a soybean ribulose-1,5-biphosphate carboxylase small subunit gene, SRS1, were investigated. RNA was labeled with [alpha-32P]UTP in nuclei isolated from plants grown under different conditions of light and darkness and used to probe Southern blots and dot blots. The levels of small subunit mRNA synthesis were normalized to ribosomal RNA synthesis. We demonstrate that the SRS1 gene is transcribed at a rate 16- to 32-fold higher in plants grown in the light than in those grown in darkness. Transcription of the small subunit increased dramatically when plants grown in darkness were given 30 min to 6 h of light and then leveled off after 24 to 48 h of exposure. When light-grown seedlings were exposed to greater than 2 h of darkness, a gradual decrease in transcription was detected. This decrease in transcription reached basal dark-grown levels after 48 h of exposure to darkness. The increase in transcription in etiolated seedlings treated with white light for 15 min could be reduced to basal levels if the treatment was followed by treatment with far-red light for 15 min. In addition, transcription in ligh-grown seedlings was reduced to basal levels when plants were exposed to far-red light for 15 min. The transcription of this ribulose-1,5-biphosphate carboxylase small subunit gene is strongly positively regulated by white light, is negatively regulated by far-red light, and exhibits a classic phytochrome-linked response.

Transcriptional regulation of a gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean tissue is linked to the phytochrome response

1985 ◽  
Vol 5 (8) ◽  
pp. 1910-1917
Author(s):  
S L Berry-Lowe ◽  
R B Meagher
Keyword(s):  
Ribosomal Rna ◽  
White Light ◽  
Red Light ◽  
Small Subunit ◽  
Subunit Gene ◽  
Gene Encoding ◽  
Bisphosphate Carboxylase ◽  
Subunit Mrna ◽  
Etiolated Seedlings ◽  
Soybean Tissue

The effects of white light, far-red light, and darkness on the transcription of a soybean ribulose-1,5-biphosphate carboxylase small subunit gene, SRS1, were investigated. RNA was labeled with [alpha-32P]UTP in nuclei isolated from plants grown under different conditions of light and darkness and used to probe Southern blots and dot blots. The levels of small subunit mRNA synthesis were normalized to ribosomal RNA synthesis. We demonstrate that the SRS1 gene is transcribed at a rate 16- to 32-fold higher in plants grown in the light than in those grown in darkness. Transcription of the small subunit increased dramatically when plants grown in darkness were given 30 min to 6 h of light and then leveled off after 24 to 48 h of exposure. When light-grown seedlings were exposed to greater than 2 h of darkness, a gradual decrease in transcription was detected. This decrease in transcription reached basal dark-grown levels after 48 h of exposure to darkness. The increase in transcription in etiolated seedlings treated with white light for 15 min could be reduced to basal levels if the treatment was followed by treatment with far-red light for 15 min. In addition, transcription in ligh-grown seedlings was reduced to basal levels when plants were exposed to far-red light for 15 min. The transcription of this ribulose-1,5-biphosphate carboxylase small subunit gene is strongly positively regulated by white light, is negatively regulated by far-red light, and exhibits a classic phytochrome-linked response.

The Rubisco small subunit gene as a paradigm for studies on differential gene expression during plant development

1986 ◽  
Vol 313 (1162) ◽  
pp. 409-417 ◽  
Keyword(s):  
Transcriptional Control ◽  
Higher Plants ◽  
Mrna Level ◽  
Small Subunit ◽  
Upstream Sequence ◽  
Specific Expression ◽  
Bisphosphate Carboxylase ◽  
Cis Acting ◽  
Transgenic Petunia ◽  
Light Inducibility

The ribulose 1,5-bisphosphate carboxylase small subunit (rbcS) in higher plants is encoded by a small multigene family. Members of the gene family contain 1-3 introns. The rbcS mRNA is differentially distributed in various plant organs. It is most abundant in leaves, less so in stems and other photosynthetic organs, and almost undetectable in roots. In leaves, the rbcS mRNA level is greatly increased by light through transcriptional control of the genes. Ti-mediated gene transfer experiments have demonstrated that the pea rbcS-E9 gene retains light-regulated expression in transformed petunia calluses and in leaves of transgenic petunia and tobacco plants. A 33-base pair sequence around the TATA box region has been shown to be involved in the light-inducibility of the rbcS-E9 gene in transformed calluses. In transgenic petunia plants, the experiments thus far have shown that 352 base pairs of 5' upstream sequence is sufficient for light-inducibility, as well as for leaf-specific expression. Further experiments in progress will help to identify and characterize other cis-acting elements involved in the differential expression of the rbcS genes.

Cis -acting elements for selective expression of two photosynthetic genes in transgenic plants

1986 ◽  
Vol 314 (1166) ◽  
pp. 493-500 ◽  
Keyword(s):  
Higher Plants ◽  
Small Subunit ◽  
Upstream Region ◽  
Organ Specificity ◽  
Base Pairs ◽  
Bisphosphate Carboxylase ◽  
Cis Acting ◽  
Conserved Sequence ◽  
Photosynthetic Genes

The most abundant mRNAs in leaves of higher plants encode the small subunit (rbcS) of ribulose 1,5-bisphosphate carboxylase and the chlorophyll a/b -binding (Cab) protein. Nuclear genes for these mRNAs are expressed in an organ-specific manner and their expression is induced by light acting through phytochrome. We have used DNA sequence manipulation in vitro coupled with transgenic plant expression systems to define sequence determinants for regulated expression of these two photosynthetic genes. At least two cis -acting elements for the rbcS genes have been identified. A conserved sequence from the transcription start site to the 5' boundary of the TATA box is involved in light-inducible transcription. In addition, an upstream enhancer-like element (240-280 base pairs) confers phytochrome responsiveness and organ specificity on constitutive promoters. An enhancer-like element ( ca . 260 base pairs) in the upstream region of the wheat Cab -1 gene also shows similar functions.

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