NItrogen fixation: Differentiating cyanobacteria rearrange their nif genes

Nature ◽  
1985 ◽  
Vol 314 (6010) ◽  
pp. 404-405 ◽  
Author(s):  
William D. P. Stewart
Keyword(s):  
1982 ◽  
Vol 149 (1) ◽  
pp. 221-228 ◽  
Author(s):  
D Corbin ◽  
G Ditta ◽  
D R Helinski

1977 ◽  
Vol 30 (2) ◽  
pp. 141 ◽  
Author(s):  
Mary L Skotnicki ◽  
Barry G Rolfe

Storage in dimethyl sulphoxide (DMSO) of Escherichia coli K12 hybrids carrying nif+ genes from Klebsiella pneumoniae can result in selection of a defective nitrogen-fixing phenotype. Similar results are obtained with E. coli K12 hybrids containing the nitrogep-fixing capacity from Rhizobium trifolii. DMSO appears to affect particular inner membrane proteins associated with energy metabolism in E. coli K12 and four chromosomal regions (chID, chlG, his and unc) are associated with resistance to DMSO.


1988 ◽  
Vol 132 (4) ◽  
pp. 405-411 ◽  
Author(s):  
Pascal Ratet ◽  
Katharina Pawlowski ◽  
Heiner Meyer Z. A. ◽  
Jeff Schell ◽  
Frans J. De Bruijn

ChemBioChem ◽  
2020 ◽  
Vol 21 (12) ◽  
pp. 1717-1722
Author(s):  
Qin Li ◽  
Sanfeng Chen
Keyword(s):  

1991 ◽  
Vol 37 (6) ◽  
pp. 425-429 ◽  
Author(s):  
Emanuel M. Souza ◽  
Shigehiro Funayama ◽  
Liu U. Rigo ◽  
Fábio O. Pedrosa

A genomic library of Herbaspirillum seropedicae was constructed and screened for the nifA gene by complementation of a nifA mutant of Azospirillum brasilense (FP10). A recombinant plasmid, pEMS1, capable of restoring acetylene reduction activity in the mutant FP10, was isolated and found to hybridize to the nifA gene of Klebsiella pneumoniae. The results suggest that nifA is involved in the regulation of nif genes in H. seropedicae. Key words: diazotroph, nitrogen fixation gene, nif regulation, complementation cloning, Herbaspirillum seropedicae.


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