scholarly journals Genetic manipulation: Rifkin strikes against gene transfer experiments

Nature ◽  
1984 ◽  
Vol 311 (5986) ◽  
pp. 495-495
Author(s):  
Tim Beardsley
Keyword(s):  
Gene Transfer ◽  
Genetic Manipulation

scholarly journals Genetic manipulation of primitive leukemic and normal hematopoietic cells using a novel method of adenovirus-mediated gene transfer

Leukemia ◽  
1999 ◽  
Vol 13 (10) ◽  
pp. 1608-1616 ◽  
Author(s):  
DS Howard ◽  
DA Rizzierri ◽  
B Grimes ◽  
D Upchurch ◽  
GL Phillips ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Genetic Manipulation ◽  
Hematopoietic Cells ◽  
Novel Method

THE POTENTIAL FOR GENETIC MANIPULATION OF PEACH FRUIT DEVELOPMENT AND QUALITY THROUGH DIRECTED GENE TRANSFER

1990 ◽  
pp. 503-508
Author(s):  
R. Scorza ◽  
P.H. Morgens ◽  
J.M. Cordts ◽  
R.A. Cohen ◽  
S. Mante ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Fruit Development ◽  
Genetic Manipulation ◽  
Peach Fruit

scholarly journals Renal Transfer of Genetically Engineered Cells

2000 ◽  
Vol 11 (suppl 2) ◽  
pp. S154-S158
Author(s):  
MASANORI KITAMURA
Keyword(s):  
Gene Transfer ◽  
Cultured Cells ◽  
Mesangial Cells ◽  
Genetic Manipulation ◽  
Ex Vivo ◽  
Genetically Engineered ◽  
Dominant Negative ◽  
Loss Of Function ◽  
Glomerular Diseases

Abstract. For many years, ex vivo gene transfer has been used for genetic manipulation of various organs. In the kidney, ex vivo gene transfer was reported using mesangial cells and macrophages. In rats, cultured cells injected into the renal artery are accumulated selectively in the glomerulus. With this approach, it is possible to transfer genetically engineered cells to normal and diseased glomeruli. The transfer of genetically engineered cells to glomeruli can be used for several purposes. With the use of resident glomerular cells engineered in vitro, it is possible to examine how the cells that overexpress certain genes behave differently in normal and diseased glomeruli. Both gain-of-function and loss-of-function strategies are useful for this purpose. For the latter, stable expression of antisense cDNA, ribosomes, or dominant-negative mutants is available. By transfer of engineered cells producing secretory, recombinant proteins, it is possible to modify glomerular microenvironment in vivo. Transfer of genes encoding therapeutically relevant molecules could be useful for therapeutic intervention. Transfer of engineered leukocytes to the glomerulus also allows investigation of cross talk between leukocytes and resident cells. Transfer of stimulated leukocytes is useful for investigation of the pathologic actions of infiltrating cells on glomerular structure and function. Leukocytes in which certain gene functions are selectively reinforced or deleted would be useful for elucidation of the exact functions of leukocyte-associated genes in glomerular diseases. This article summarizes current experience with the adoptive transfer of engineered cells to the glomerulus for investigation of and therapy for glomerular diseases.

Prospects for the molecular genetic manipulation of livestock

1992 ◽  
Vol 1992 ◽  
pp. 7-7
Author(s):  
Ian Wilmut
Keyword(s):  
Gene Transfer ◽  
Genetic Manipulation ◽  
Molecular Genetic ◽  
Target Population ◽  
Early Embryo ◽  
Animal Production ◽  
Farm Animals ◽  
Effective Scheme ◽  
Pharmaceutical Proteins ◽  
Repair Mechanisms

Molecular genetic manipulation can now be used to produce pharmaceutical proteins in the milk of farm animals. In the longer term this technology will be used to modify aspects of animal production, but it is not clear how many manipulations will be useful nor when such applications will become practicable. Improvements are required in all four aspects of an effective scheme for either gene transfer or targeting: it must be possible 1). to make the change, 2). to regulate expression of the gene in the desired manner, 3). to identify genes that are able to have a significant effect and 4). to disseminate the change into the target population.There is only one method that has been used to transfer a gene in livestock and this depends upon injection of a few hundred copies of the gene into a nucleus in the early embryo. It is believed that integration occurs because the act of injecting fluid causes breakages in chromosomes and that the repair mechanisms sometimes include injected DNA. There are serious implications.

Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology

2000 ◽  
Vol 279 (1) ◽  
pp. H429-H436 ◽  
Author(s):  
Ying-Ying Zhou ◽  
Shi-Qiang Wang ◽  
Wei-Zhong Zhu ◽  
Andrej Chruscinski ◽  
Brian K. Kobilka ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Adult Mouse ◽  
Biochemical Characterization ◽  
Genetic Manipulation ◽  
Rapid Development ◽  
High Yield ◽  
Double Knockout ◽  
Cardiovascular Research ◽  
Adenoviral Infection ◽  
Vector Systems

Rapid development of transgenic and gene-targeted mice and acute genetic manipulation via gene transfer vector systems have provided powerful tools for cardiovascular research. To facilitate the phenotyping of genetically engineered murine models at the cellular and subcellular levels and to implement acute gene transfer techniques in single mouse cardiomyocytes, we have modified and improved current enzymatic methods to isolate a high yield of high-quality adult mouse myocytes (5.3 ± 0.5 × 105 cells/left ventricle, 83.8 ± 2.5% rod shaped). We have also developed a technique to culture these isolated myocytes while maintaining their morphological integrity for 2–3 days. The high percentage of viable myocytes after 1 day in culture (72.5 ± 2.3%) permitted both physiological and biochemical characterization. The major functional aspects of these cells, including excitation-contraction coupling and receptor-mediated signaling, remained intact, but the contraction kinetics were significantly slowed. Furthermore, gene delivery via recombinant adenoviral infection was highly efficient and reproducible. In adult β1/β2-adrenergic receptor (AR) double-knockout mouse myocytes, adenovirus-directed expression of either β1- or β2-AR, which occurred in 100% of cells, rescued the functional response to β-AR agonist stimulation. These techniques will permit novel experimental settings for cellular genetic physiology.

Strain selection and improvement of gene transfer for genetic manipulation of Pseudomonas savastanoi isolated from olive knots

2007 ◽  
Vol 158 (1) ◽  
pp. 60-69 ◽  
Author(s):  
Isabel Pérez-Martínez ◽  
Luis Rodriguez-Moreno ◽  
Isabel María Matas ◽  
Cayo Ramos
Keyword(s):  
Gene Transfer ◽  
Genetic Manipulation ◽  
Strain Selection
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