Role for mouse macrophage IgG Fc receptor as ligand-dependent ion channel

Nature ◽  
1983 ◽  
Vol 306 (5939) ◽  
pp. 186-189 ◽  
Author(s):  
John Ding-E Young ◽  
Jay C. Unkeless ◽  
Tsen M. Young ◽  
Alexander Mauro ◽  
Zanvil A. Cohn
Keyword(s):  
Ion Channel ◽  
Fc Receptor ◽  
Mouse Macrophage

Functional ion channel formation by mouse macrophage IgG Fc receptor triggered by specific ligands

1985 ◽  
Vol 29 (4) ◽  
pp. 289-297 ◽  
Author(s):  
John Ding-E Young ◽  
Jay C. Unkeless ◽  
Zanvil A. Cohn
Keyword(s):  
Ion Channel ◽  
Fc Receptor ◽  
Mouse Macrophage ◽  
Channel Formation

scholarly journals Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors.

1979 ◽  
Vol 150 (3) ◽  
pp. 580-596 ◽  
Author(s):  
J C Unkeless
Keyword(s):  
Monoclonal Antibody ◽  
Cell Lines ◽  
Fc Receptors ◽  
Inhibitory Effect ◽  
Fc Receptor ◽  
Antigenic Relationship ◽  
Mouse Macrophage ◽  
Spleen Cells ◽  
Binding Studies ◽  
Fab Fragment

To investigate the antigenic relationship between the macrophage and lymphocyte Fc receptors (FcR), a monoclonal antibody capable of blocking mouse macrophage Fc receptors was selected. Hybrids were formed by fusing the P3U1 mouse myeloma and spleen cells from a rat immunized with the mouse macrophage-like cell lines J774 and P388D1. The Fab fragment of the monoclonal IgG secreted by clone 2.4G2, inhibited the trypsin-resistant Fc receptor II (FcRII), which is specific for immune aggregates of mouse IgG1 and IgG2b, but had no inhibitory effect on the trypsin-sensitive Fc receptor I (FcRI), which binds monomeric IgG2a and erythrocytes coated with IgG2a. Thus, the monoclonal 2.4G2 IgG appeared to be specific for macrophage FcRII. Further evidence that the 2.4G2 IgG was directed against FcRII came from binding studies of the monoclonal antibody to J774 cells and a series of independently isolated variants which do not express FcRII. These variants of J774 bound 5% as much of the monoclonal antibody as the parent line, which bound 600,000 molecules of 2.4G2 IgG per cell. The antigenic relatedness of mouse lymphocyte FcR to mouse macrophage FcRII was demonstrated by the binding of 2.4G2 IgG to FcR-bearing lymphoid cell lines and the inhibition of the lymphocyte FcR by the monoclonal antibody. Preincubation of spleen cells and peritioneal cells with 2.3G2 IgG likewise inhibited rosette formation with ox erythrocytes coated with rabbit IgG. The ability of the hybridoma IgG to inhibit mouse FcRII was independent of the major histocompatibility complex. The 2.4G2 IgG antigenic determinant was not present on rat, guinea pig, rabbit, or human FcR-bearing cells.

scholarly journals Fc-receptor variants of a mouse macrophage cell line

1979 ◽  
Vol 76 (3) ◽  
pp. 1400-1404 ◽  
Author(s):  
J. C. Unkeless ◽  
G. Kaplan ◽  
H. Plutner ◽  
Z. A. Cohn
Keyword(s):  
Cell Line ◽  
Fc Receptor ◽  
Mouse Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Mouse Macrophage Cell Line

Ultrastructural study of cytoskeletal interactions with endosomes in macrophages by quickfreezing and deep-etching method

1990 ◽  
Vol 48 (3) ◽  
pp. 576-577
Author(s):  
Takeshi Baba ◽  
Nobuki Shiozawa ◽  
Masao Hotch ◽  
Shinichi Ohno
Keyword(s):  
Immune Complex ◽  
Ultrastructural Study ◽  
Fc Receptor ◽  
Coated Pits ◽  
Deep Etching ◽  
Receptor Mediated Endocytosis ◽  
Etching Method ◽  
Quick Freezing

Endosomes are vesicular or tubular organelles that play important roles in transports of receptors and receptor―bound ligands during receptor-mediated endocytosis. The mechanisms of endocytic transports from clathrin-coated pits to lysosomes have been studied by many investigators. However, few studies were reported about the interactions between endosomes and cytoskeletons. In this study, Fc-receptor-mediated endocytosis in macrophages are investigated by quick-freezing and deep-etching (QF-DE) method combined with gold-labeled immune complex and “replica scraping method”.

Myrsinane and related diterpenes from Euphorbia falcata with selective potassium ion channel activity

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
A Vasas ◽  
P Orvos ◽  
L Tálosi ◽  
P Forgo ◽  
G Pinke ◽  
...  
Keyword(s):  
Ion Channel ◽  
Potassium Ion ◽  
Channel Activity ◽  
Potassium Ion Channel
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