A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation

Nature ◽  
1983 ◽  
Vol 304 (5922) ◽  
pp. 184-187 ◽  
Author(s):  
Michael W. Bevan ◽  
Richard B. Flavell ◽  
Mary-Dell Chilton
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Plant Cell ◽  
Cell Transformation ◽  
Selectable Marker ◽  
Antibiotic Resistance Gene ◽  
Plant Cell Transformation

scholarly journals Bleomycin resistance: a new dominant selectable marker for plant cell transformation

1986 ◽  
Vol 7 (3) ◽  
pp. 171-176 ◽  
Author(s):  
Jacques Hille ◽  
Frank Verheggen ◽  
Peter Roelvink ◽  
Henk Franssen ◽  
Ab van Kammen ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Transformation ◽  
Selectable Marker ◽  
Dominant Selectable Marker ◽  
Plant Cell Transformation

Phleomycin resistance as a dominant selectable marker for plant cell transformation

1989 ◽  
Vol 13 (4) ◽  
pp. 365-373 ◽  
Author(s):  
Pascual Perez ◽  
G�rard Tiraby ◽  
Jean Kallerhoff ◽  
Jo�l Perret
Keyword(s):  
Plant Cell ◽  
Cell Transformation ◽  
Selectable Marker ◽  
Dominant Selectable Marker ◽  
Plant Cell Transformation

scholarly journals An Efficient Method of Selectable Marker Gene Excision by Xer Recombination for Gene Replacement in Bacterial Chromosomes

2006 ◽  
Vol 72 (4) ◽  
pp. 2520-2525 ◽  
Author(s):  
Alexandra E. Bloor ◽  
Rocky M. Cranenburgh
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Selectable Marker ◽  
Antibiotic Resistance Gene ◽  
Marker Gene ◽  
Stable Gene ◽  
Selectable Marker Gene ◽  
Site Specific ◽  
Target Locus ◽  
Bacterial Chromosomes

ABSTRACT A simple, effective method of unlabeled, stable gene insertion into bacterial chromosomes has been developed. This utilizes an insertion cassette consisting of an antibiotic resistance gene flanked by dif sites and regions homologous to the chromosomal target locus. dif is the recognition sequence for the native Xer site-specific recombinases responsible for chromosome and plasmid dimer resolution: XerC/XerD in Escherichia coli and RipX/CodV in Bacillus subtilis. Following integration of the insertion cassette into the chromosomal target locus by homologous recombination, these recombinases act to resolve the two directly repeated dif sites to a single site, thus excising the antibiotic resistance gene. Previous approaches have required the inclusion of exogenous site-specific recombinases or transposases in trans; our strategy demonstrates that this is unnecessary, since an effective recombination system is already present in bacteria. The high recombination frequency makes the inclusion of a counter-selectable marker gene unnecessary.

scholarly journals Metagenomics Analysis Reveals the Microbial Communities, Antimicrobial Resistance Gene Diversity and Potential Pathogen Transmission Risk of Two Different Landfills in China

Diversity ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 230
Author(s):  
Shan Wan ◽  
Min Xia ◽  
Jie Tao ◽  
Yanjun Pang ◽  
Fugen Yu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Microbial Communities ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Gene Diversity ◽  
Antibiotic Resistance Gene ◽  
Antibiotic Resistance Genes ◽  
Pathogen Transmission ◽  
Transmission Risk ◽  
Antimicrobial Resistance Gene

In this study, we used a metagenomic approach to analyze microbial communities, antibiotic resistance gene diversity, and human pathogenic bacterium composition in two typical landfills in China. Results showed that the phyla Proteobacteria, Bacteroidetes, and Actinobacteria were predominant in the two landfills, and archaea and fungi were also detected. The genera Methanoculleus, Lysobacter, and Pseudomonas were predominantly present in all samples. sul2, sul1, tetX, and adeF were the four most abundant antibiotic resistance genes. Sixty-nine bacterial pathogens were identified from the two landfills, with Klebsiella pneumoniae, Bordetella pertussis, Pseudomonas aeruginosa, and Bacillus cereus as the major pathogenic microorganisms, indicating the existence of potential environmental risk in landfills. In addition, KEGG pathway analysis indicated the presence of antibiotic resistance genes typically associated with human antibiotic resistance bacterial strains. These results provide insights into the risk of pathogens in landfills, which is important for controlling the potential secondary transmission of pathogens and reducing workers’ health risk during landfill excavation.

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