High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies

Nature ◽  
1982 ◽  
Vol 300 (5890) ◽  
pp. 374-376 ◽  
Author(s):  
Bellur S. Prabhakar ◽  
Martin V. Haspel ◽  
Patrick R. McClintock ◽  
Abner Louis Notkins
2021 ◽  
pp. 1-37
Author(s):  
Christian Schoning ◽  
Jørn Helder ◽  
Chloé Diskin-Holdaway

Abstract The last three decades have witnessed increasing interest in discourse-pragmatic markers (DPMs), both with regards to their high frequency in spoken discourse and their multifunctionality in interaction. Most studies have centered on English, with studies on Danish restricted to a handful of previous interactional discourse analyses. This paper is a preliminary investigation of the Danish word sådan (commonly glossed as ‘such’ or ‘like this/that’). A qualitative, form-based, discourse analytic approach is undertaken on over 40 minutes of naturally occurring Danish talk to argue that sådan qualifies as a DPM. In service of textual, subjective, and intersubjective macro-functions, sådan illustrates; exemplifies; marks hesitation; approximates a quantity; mitigates, hedges, or softens; and allows self-correction or self-repair. These findings are discussed in terms of their implications for sådan’s place in the Danish DPM system and our understanding of DPMs across languages.


2011 ◽  
Vol 15 (3) ◽  
pp. 523-547 ◽  
Author(s):  
LYNN CLARK ◽  
KEVIN WATSON

The variable phenomenon in which /t/ can be realized as a tap or rhotic approximant in varieties of Northern British English (commonly referred to as t-to-r, Wells 1982: 370) has received some attention in English linguistics as debates have appeared over how best to model its phonology (e.g. Carr 1991; Docherty et al. 1997; Broadbent 2008). The occurrence of t-to-r seems to be constrained by the preceding and following phonological environment in a largely systematic way and so it is often accounted for within a rule-based model of grammar. Problematically, however, the rule does not apply blindly across the board to all words which fit the specified phonological pattern. Instead, t-to-r shows evidence of being lexically restricted, and this fact has recently encouraged a usage-based interpretation. Until now, there has been relatively little attempt to test the usage-based thesis directly with fully quantified data gleaned from naturally occurring conversation. This article investigates the extent to which certain usage-based predictions can account for variation attested in t-to-r in Liverpool English. Using oral history interviews with Liverpool English speakers born in the early 1900s, we examine the usage-based predictions first proposed by Broadbent (2008) that t-to-r is more likely in (a) high-frequency words and (b) high-frequency phrases. There is some support for the importance of lexical frequency as a motivating factor in the use of t-to-r, but our data do not fully support either of these claims wholesale. We suggest that t-to-r is not constrained simply by word frequency or phrase frequency alone, but by a combination of both. Finally, we explore the possibility of employing notions from Cognitive Grammar such as schema strength (e.g. Taylor 2002; Bybee 1995: 430) in our interpretation of these data.


1988 ◽  
Vol 256 (2) ◽  
pp. 661-664 ◽  
Author(s):  
M S Stoll ◽  
T Mizuochi ◽  
R A Childs ◽  
T Feizi

Conditions have been established for the rapid and efficient conjugation of reducing oligosaccharides (di- to deca-saccharides) to dipalmitoyl phosphatidylethanolamine. The resulting neoglycolipids derived from several naturally occurring oligosaccharides and a series of N-linked high-mannose-type oligosaccharides released by hydrazinolysis from RNAase B showed specific and potent reactivities, as appropriate, with monoclonal antibodies to blood group Lewis(b), blood group A or a stage-specific embryonic (SSEA-1) antigen, or the lectin concanavalin A.


1997 ◽  
Vol 52 (5-6) ◽  
pp. 391-395
Author(s):  
Juan José López-Moya ◽  
Dionisio López-Abella ◽  
José-Ramón Díaz-Rúiz ◽  
Belén Martinez-Garcia ◽  
Richard Gáborjányi

Abstract Three Hungarian (No.2, 4 and 9), and a Moldavian (K) plum pox virus isolates were compared with a characterized Spanish isolate (5.15) by RT-PCR, ELISA, dot-blot and West­ern blot analysis. Monoclonal antibodies prepared against the external, intermediate and internal sequences of the coat protein of the Spanish isolate were able to differentiate the four isolates. Hungarian isolate No. 2 proved to be serologically identical to the Spanish isolate, while No. 4 showed appreciable differences and No. 9 could be recognized only by the monoclonal antibodies representing the intermedial and internal parts of the coat protein. K isolate showed a more distant relationship to other isolates. Our experiment provided the first demonstration of the presence of D type isolates in Hungary.


1966 ◽  
Vol 3 (5) ◽  
pp. 556-564 ◽  
Author(s):  
N. F. Cheville

Five virus isolates were obtained from pox-like lesions in swine. The respective skin lesions, infected cell cultures, virus particles and experimentally infected young swine were identical to that of swinepox by histologic examination, immunofluorescence and electron microscopy. The fluorescent antibody test provided a rapid and accurate means of diagnosis of naturally occurring lesions.


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