Crystal structure of the spliceosomal U2B″–U2A′ protein complex bound to a fragment of U2 small nuclear RNA

Nature ◽  
10.1038/29234 ◽  
1998 ◽  
Vol 394 (6694) ◽  
pp. 645-650 ◽  
Author(s):  
Stephen R. Price ◽  
Philip R. Evans ◽  
Kiyoshi Nagai
Keyword(s):  
Crystal Structure ◽  
Protein Complex ◽  
Small Nuclear Rna ◽  
Nuclear Rna

The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro

Cell ◽  
1983 ◽  
Vol 33 (2) ◽  
pp. 509-518 ◽  
Author(s):  
Stephen M. Mount ◽  
Ingvar Pettersson ◽  
Monique Hinterberger ◽  
Aavo Karmas ◽  
Joan A. Steitz
Keyword(s):  
Splice Site ◽  
Protein Complex ◽  
Small Nuclear Rna ◽  
Nuclear Rna

scholarly journals A TFIIH-Associated Mediator Head Is a Basal Factor of Small Nuclear Spliced Leader RNA Gene Transcription in Early-Diverged Trypanosomes

2010 ◽  
Vol 30 (23) ◽  
pp. 5502-5513 ◽  
Author(s):  
Ju Huck Lee ◽  
Gang Cai ◽  
Aswini K. Panigrahi ◽  
Star Dunham-Ems ◽  
Tu N. Nguyen ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Protein Complex ◽  
Complex Form ◽  
Amino Acid Sequences ◽  
Small Nuclear Rna ◽  
Basal Transcription ◽  
Spliced Leader ◽  
Nuclear Rna

ABSTRACT Genome annotation suggested that early-diverged kinetoplastids possess a reduced set of basal transcription factors. More recent work, however, on the lethal parasite Trypanosoma brucei identified extremely divergent orthologs of TBP, TFIIA, TFIIB, and TFIIH which, together with the small nuclear RNA-activating protein complex, form a transcription preinitiation complex (PIC) at the spliced leader (SL) RNA gene (SLRNA) promoter. The SL RNA is a small nuclear RNA and a trans splicing substrate for the maturation of all pre-mRNAs which is metabolized continuously to sustain gene expression. Here, we identified and biochemically characterized a novel TFIIH-associated protein complex in T. brucei (Med-T) consisting of nine subunits whose amino acid sequences are conserved only among kinetoplastid organisms. Functional analyses in vivo and in vitro demonstrated that the complex is essential for cell viability, SLRNA transcription, and PIC integrity. Molecular structure analysis of purified Med-T and Med-T/TFIIH complexes by electron microscopy revealed that Med-T corresponds to the mediator head module of higher eukaryotes. These data therefore show that mediator is a basal factor for small nuclear SL RNA gene transcription in trypanosomes and that the basal transcription function of mediator head is a characteristic feature of eukaryotes which developed early in their evolution.

scholarly journals Aberrant 3′ oligoadenylation of spliceosomal U6 small nuclear RNA in poikiloderma with neutropenia

Blood ◽  
2013 ◽  
Vol 121 (6) ◽  
pp. 1028-1038 ◽  
Author(s):  
Christine Hilcenko ◽  
Paul J. Simpson ◽  
Andrew J. Finch ◽  
Frank R. Bowler ◽  
Mark J. Churcher ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Small Nuclear Rna ◽  
Key Points ◽  
Nuclear Rna ◽  
Poikiloderma With Neutropenia

Key Points Crystal structure of human USB1 identifies it as a member of the LigT-like superfamily of 2H phosphoesterases. USB1 protects spliceosomal U6 small nuclear RNA from aberrant 3′ oligoadenylation.

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